Study of Holtermanniella wattica, Leucosporidium creatinivorum, Naganishia adeliensis, Solicoccozyma aeria, and Solicoccozyma terricola for their lipogenic aptitude from different carbon sources

Abstract

BackgroundThe ability of some microorganisms to accumulate lipids is well known; however, only recently the number of studies on microbial lipid biosynthesis for obtaining oleochemical products, namely biofuels and some building blocks for chemistry, is rapidly and spectacularly increased. Since 1990s, some oleaginous yeasts were studied for their ability to accumulate lipids up to 60–70% of their dry weight. Due to the vast array of engineering techniques currently available, the recombinant DNA technology was the main approach followed so far for obtaining lipid-overproducing yeasts, mainly belonging to the Yarrowia lipolytica. However, an alternative approach can be offered by worldwide diversity as source of novel oleaginous yeasts. Lipogenic aptitude of a number of yeast strains has been reviewed, but many of these studies utilized a limited number of species and/or different culture conditions that make impossible the comparison of different results. Accordingly, the lipogenic aptitude inside the yeast world is still far from being fully explored, and finding new oleaginous yeast species can acquire a strategic importance.Results Holtermanniella wattica, Leucosporidium creatinivorum, Naganishia adeliensis, Solicoccozyma aeria, and Solicoccozyma terricola strains were selected as a result of a large-scale screening on 706 yeasts (both Ascomycota and Basidiomycota). Lipid yields and fatty acid profiles of selected strains were evaluated at 20 and 25 °C on glucose, and on glycerol, xylose, galactose, sucrose, maltose, and cellobiose. A variable fatty acid profile was observed in dependence of both temperature and different carbon sources. On the whole, L. creatinivorum exhibited the highest performances: total lipid yield (YL) >7 g/l on glucose and glycerol, % of intracellular lipids on cell biomass (YL/DW) >70% at 20 °C on glucose, lipid coefficient (YL/Glu) around 20% on glucose, and daily productivity (YL/d) on glucose and sucrose >1.6 g/(l*d).ConclusionsThis study provides some meaningful information about the lipogenic ability of some yeast species. Variable lipid yields and fatty acid profiles were observed in dependence of both temperature and different carbon sources. L. creatinivorum exhibited the highest lipogenic performances.Electronic supplementary materialThe online version of this article (doi:10.1186/s13068-016-0672-1) contains supplementary material, which is available to authorized users.

Effects of Agricultural Management on Rhizosphere Microbial Structure and Function in Processing Tomato Plants

“…3b). Holtermanniella is a small, cold-tolerant genus of potentially parasitic fungi (38) that includes species able to metabolize diverse carbon compounds and generate unique fatty acid profiles (39).…”

Yeast lipids from cardoon stalks, stranded driftwood and olive tree pruning residues as possible extra sources of oils for producing biofuels and biochemicals

“…Leucosporidium creatinivorum DBVPG 4794, Naganishia adeliensis DBVPG 5195 and Solicoccozyma terricola DBVPG 5870 were used. They were previously selected on the basis of their superior lipogenic aptitude [ 13 ] and were preserved at − 80 °C in the Industrial Yeast Collection DBVPG of the Department of Agricultural, Food and Environmental Sciences, University of Perugia, Italy. Salient information on strains is reported on the DBVPG website ( http://www.dbvpg.unipg.it ).…”

“…Recombinant strains of Yarrowia lipolytica have been extensively studied for obtaining high lipid productions [ 9 – 11 ]. However, some recent studies found that some basidiomycetous yeasts can be considered as possible alternatives to conventional oleaginous yeasts due to their high aptitude to accumulate lipids [ 8 , 12 , 13 ].…”

Produção de L-asparaginase pela levedura >i<Leucosporidium muscorum>/i< CRM 1648 isolada de sedimento marinho coletado na Península Antártica

“…A triagem e seleção dos componentes do meio de cultivo são importantes para tornar o processo de produção economicamente viável, principalmente porque essa composição é específica para cada organismo produtor (GHOLAMIAN et al, 2013 (MAŠÍNOVÁ et al, 2017;FILIPPUCCI et al, 2016;WANG et al, 2015 (GUTIÉRREZ-ALONSO et al, 2009) e R.…”

“…que mostrou crescimento também em meio com xilose e maltose, mas não foi capaz de crescer em galactose e celobiose(FILIPPUCCI et al, 2016). Em outro estudo, L. muscorum foi capaz de assimilar sorbose, xilose, lactose e rafinose(LAICH et al, 2014).…”