Study of human acellular amniotic membrane loading bone marrow mesenchymal stem cells in repair of articular cartilage defect in rabbits

Liu, P.-F.; Guo, Likun; Zhao, Ding-Yun; Zhang, Z.-J.; Kang, Kyung Ku; Zhu, Rui; Xiao-ying, Yuan

doi:10.4238/2014.september.29.12

Cited by 26 publications

(17 citation statements)

References 20 publications

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“…Thus, AHAM provide a favourable environment for the in‐migration, proliferation, and differentiation of chondrocytes and epithelial cells. Our findings are consistent with previous reports showing that AHAM engraftment improves the repair of articular cartilage defects in rabbits through the enhanced cultivation of bone marrow mesenchymal stem cells (Liu et al, ).…”

Section: Discussionsupporting

confidence: 94%

Tracheal repair with acellular human amniotic membrane in a rabbit model

Jorge

Francisco²,

Bergonse

et al. 2017

J Tissue Eng Regen Med

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Surgical correction of tracheal stenosis is still a complex and challenging procedure. Acellular human amniotic membranes (AHAM) represent a promising biomaterial source for tissue regeneration. The aim of this study was to evaluate whether AHAM grafts improve tissue regeneration of the trachea in a rabbit model of tracheostomy. Twenty rabbits were randomized into 2 groups. Animals in the control group underwent surgical tracheostomy only, and animals in the AHAM group underwent surgical tracheostomy and received an AHAM graft that covered the defect site. We examined tissues at the site of tracheostomy 60 days after surgery by histological analysis with haematoxylin and eosin, Movat's pentachrome stain and immunohistochemistry by analysis with antiaggrecan antibodies. The average perimeter and area of the defect 60 days after surgery were smaller in animals in the control group than in the AHAM group (p = .011 and p = .011, respectively). Histological analysis of AHAM group revealed neovascularization, islands of immature cartilage, pseudostratified ciliated epithelium. and connective tissue at the site of AHAM engraftment, whereas only pseudostratified ciliated epithelium and connective tissue were observed at the defect site in tissues of animals in the control group. Regeneration of islands of immature cartilage tissue with hyaline pattern and pseudostratified ciliated epithelium were confirmed by immunohistochemistry analysis. These results indicate that AHAM engraftment could facilitate neovascularization and regeneration of immature cartilage in a model of tracheal injury. Its use may lower the risk of post-operative complications including stenosis of trachea.

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Section: Discussionsupporting

confidence: 94%

Tracheal repair with acellular human amniotic membrane in a rabbit model

Jorge

Francisco²,

Bergonse

et al. 2017

J Tissue Eng Regen Med

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“…Of note, the combination of amniotic membrane treatment and MSCs has shown better therapeutic effects than amniotic membrane treatment alone in patients with ocular chemical burns, skin defects, and articular cartilage defects. 47,48 Thus for some severe ocular disorders, the combination of amniotic membrane treatment and MSCs or MSC-CMT might be a promising therapy.…”

Section: Discussionmentioning

confidence: 99%

Culture medium from TNF-α–stimulated mesenchymal stem cells attenuates allergic conjunctivitis through multiple antiallergic mechanisms

Wan

Huang

et al. 2015

Journal of Allergy and Clinical Immunology

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“…So far, the HAAM scaffold has been used in many fields, especially in repairing skin defects [45]. In recent years, an increasing number of studies have found that the HAAM scaffold had a positive effect on orthopedic diseases, such as promoting bone regeneration and repairing articular cartilage defects [46,47].…”

Section: Discussionmentioning

confidence: 99%

“…In comparison with other methods, this method offered many advantages, such as quicker results, simpler process, and lower cost. Although this method to prepare the HAAM scaffold has been used in other studies [47,50], we reduced the duration taken for enzymatic digestion, improving the integrity of the extracellular matrix and reducing time and cost. HE staining was performed on fresh HAM and HAAM scaffolds in order to confirm thorough decellularization of HAM.…”

Section: Discussionmentioning

confidence: 99%

FGF-2-Induced Human Amniotic Mesenchymal Stem Cells Seeded on a Human Acellular Amniotic Membrane Scaffold Accelerated Tendon-to-Bone Healing in a Rabbit Extra-Articular Model

Zhang

Liu

et al. 2020

Stem Cells International

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Background. FGF-2 (basic fibroblast growth factor) has a positive effect on the proliferation and differentiation of many kinds of MSCs. Therefore, it represents an ideal molecule to facilitate tendon-to-bone healing. Nonetheless, no studies have investigated the application of FGF-2-induced human amniotic mesenchymal stem cells (hAMSCs) to accelerate tendon-to-bone healing in vivo. Objective. The purpose of this study was to explore the effect of FGF-2 on chondrogenic differentiation of hAMSCs in vitro and the effect of FGF-2-induced hAMSCs combined with a human acellular amniotic membrane (HAAM) scaffold on tendon-to-bone healing in vivo. Methods. In vitro, hAMSCs were transfected with a lentivirus carrying the FGF-2 gene, and the potential for chondrogenic differentiation of hAMSCs induced by the FGF-2 gene was assessed using immunofluorescence and toluidine blue (TB) staining. HAAM scaffold was prepared, and hematoxylin and eosin (HE) staining and scanning electron microscopy (SEM) were used to observe the microstructure of the HAAM scaffold. hAMSCs transfected with and without FGF-2 were seeded on the HAAM scaffold at a density of 3 × 10 5 cells/well. Immunofluorescence staining of vimentin and phalloidin staining were used to confirm cell adherence and growth on the HAAM scaffold. In vivo, the rabbit extra-articular tendon-to-bone healing model was created using the right hind limb of 40 New Zealand White rabbits. Grafts mimicking tendon-to-bone interface (TBI) injury were created and subjected to treatment with the HAAM scaffold loaded with FGF-2induced hAMSCs, HAAM scaffold loaded with hAMSCs only, HAAM scaffold, and no special treatment. Macroscopic observation, imageological analysis, histological assessment, and biomechanical analysis were conducted to evaluate tendon-tobone healing after 3 months. Results. In vitro, cartilage-specific marker staining was positive for the FGF-2 overexpression group. The HAAM scaffold displayed a netted structure and mass extracellular matrix structure. hAMSCs or hAMSCs transfected with FGF-2 survived on the HAAM scaffold and grew well. In vivo, the group treated with HAAM scaffold loaded with FGF-2-induced hAMSCs had the narrowest bone tunnel after three months as compared with other groups. In addition, macroscopic and histological scores were higher for this group than for the other groups, along with the best mechanical strength. Conclusion. hAMSCs transfected with FGF-2 combined with the HAAM scaffold could accelerate tendon-to-bone healing in a rabbit extraarticular model.

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Study of human acellular amniotic membrane loading bone marrow mesenchymal stem cells in repair of articular cartilage defect in rabbits

Cited by 26 publications

References 20 publications

Tracheal repair with acellular human amniotic membrane in a rabbit model

Tracheal repair with acellular human amniotic membrane in a rabbit model

Culture medium from TNF-α–stimulated mesenchymal stem cells attenuates allergic conjunctivitis through multiple antiallergic mechanisms

FGF-2-Induced Human Amniotic Mesenchymal Stem Cells Seeded on a Human Acellular Amniotic Membrane Scaffold Accelerated Tendon-to-Bone Healing in a Rabbit Extra-Articular Model

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