Study of human microecology by mass spectrometry of microbial markers

Осипов, Г. А.; Верховцева, Н. В.

doi:10.3920/bm2010.0017

Cited by 18 publications

(9 citation statements)

References 17 publications

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“…B. fragilis associates with the colonic mucin, which would facilitate absorption, and has been implicated in risk of colon cancer (197–199). Of relevance to their use as biomarkers of bacterial exposures, blood measures of bacterial fatty acids have been shown to reflect the microbes present in the intestinal tract (200). …”

Section: Biomarkers Of Systemic Bacterial Exposuresmentioning

confidence: 99%

Obesity-associated cancer risk: the role of intestinal microbiota in the etiology of the host proinflammatory state

Djurić

2017

Translational Research

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Obesity increases the risks of many cancers. One important mechanism behind this association is the obesity-associated pro-inflammatory state. Although the composition of the intestinal microbiome undoubtedly can contribute to the pro-inflammatory state, perhaps the most important aspect of host-microbiome interactions is host exposure to components of intestinal bacteria that stimulate the inflammatory reactions. Systemic exposures to intestinal bacteria can be modulated by dietary factors by altering both the composition of the intestinal microbiota as well as absorption of bacterial products from the intestinal lumen. In particular, high fat and high energy diets have been shown to facilitate absorption of bacterial lipopolysaccharide (LPS) from intestinal bacteria. Biomarkers of bacterial exposures that have been measured in blood include LPS-binding protein, sCD14, fatty acids characteristic of intestinal bacteria and immunoglobulins specific for bacterial LPS and flagellin. The optimal strategies to reduce these pro-inflammatory exposures, whether by altering diet composition, avoiding a positive energy balance or reducing adipose stores, likely differ in each individual. Biomarkers that assess systemic bacterial exposures therefore should be useful to optimize and personalize preventive approaches for individuals and groups with specific characteristics, and to gain insight into the possible mechanisms involved with different preventive approaches.

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Section: Biomarkers Of Systemic Bacterial Exposuresmentioning

confidence: 99%

Obesity-associated cancer risk: the role of intestinal microbiota in the etiology of the host proinflammatory state

Djurić

2017

Translational Research

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“…It is of note that main management in all these studies was orally-administrated Lactobacillus which may not be a good choice because of the low proportion of Lactobacillus in pharyngeal or respiratory tract microbiomes [61] . In addition, effective administration methods that can detain probiotics in the pharynx and multiple probiotic regimes may further improve the overall treatment outcome [62] . Therefore, there are no straightforward conclusions regarding the efficacy of probiotics for treatment of RTIs, based on the findings of current reports.…”

Section: Restoration Of the Pharyngeal Microbiomementioning

confidence: 99%

Human Pharyngeal Microbiome May Play A Protective Role in Respiratory Tract Infections

Gao

et al. 2014

Genomics, Proteomics &Amp; Bioinformatics

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The human pharyngeal microbiome, which resides at the juncture of digestive and respiratory tracts, may have an active role in the prevention of respiratory tract infections, similar to the actions of the intestinal microbiome against enteric infections. Recent studies have demonstrated that the pharyngeal microbiome comprises an abundance of bacterial species that interacts with the local epithelial and immune cells, and together, they form a unique micro-ecological system. Most of the microbial species in microbiomes are obligate symbionts constantly adapting to their unique surroundings. Indigenous commensal species are capable of both maintaining dominance and evoking host immune responses to eliminate invading species. Temporary damage to the pharyngeal microbiome due to the impaired local epithelia is also considered an important predisposing risk factor for infections. Therefore, reinforcement of microbiome homeostasis to prevent invasion of infection-prone species would provide a novel treatment strategy in addition to antibiotic treatment and vaccination. Hence continued research efforts on evaluating probiotic treatment and developing appropriate procedures are necessary to both prevent and treat respiratory infections.

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“…The chromatographic analysis of the microbial intestinal chyme community was performed by determining the polar fatty acid (PLFA) composition as microbial markers using gas chromatography mass spectrometry (GC-MS) (Agilent Technologies, USA) [13,14]. Lipid components of the chyme content of chickens was extracted by acid methanolysis in 0.4 ml of 1 M NaCl in methanol for one hour at a temperature of 80°C.…”

Section: Analysis Of Microbial Diversitymentioning

confidence: 99%

“…The temperature of the injector was 280 °C, the temperature of the interface was 250 °C. The obtained chromatograms were processed using a standard data processing program and a report was formed on the composition of the microbial community reconstructed by using markers [14].…”

Section: Analysis Of Microbial Diversitymentioning

confidence: 99%

Probiotic potential of Escherichia coli ŽP for the gut microbiota of chickens

et al. 2020

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The effect of oral administration of Escherichia coli ŽP strain carrying a conjugative plasmid encoding the colicin E7 on the composition of the intestinal chyme microbiota of chickens was studied. It was shown that E. coli ŽP was present in the chyme of chickens 6-24 days of age in all experimental groups. In the microbiota of the control group (not administered with the ŽP strain) the Firmicutes phylum, as well as Proteobacteria (E. coli), Actinobacteria (Eubacterium spp.) and, to a lesser extent, Bacteroidetes were found. In the experimental groups the proportion of Firmicutes, represented by the Lactobacillus, Bacillus and Clostridium genera, was higher than in the control group. Our results showed that the use of E. coli ŽP helps to maintain a dynamic state of the microbiota, in all experimental groups, including the group, which was administered an APEC strain.

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Study of human microecology by mass spectrometry of microbial markers

Cited by 18 publications

References 17 publications

Obesity-associated cancer risk: the role of intestinal microbiota in the etiology of the host proinflammatory state

Obesity-associated cancer risk: the role of intestinal microbiota in the etiology of the host proinflammatory state

Human Pharyngeal Microbiome May Play A Protective Role in Respiratory Tract Infections

Probiotic potential of Escherichia coli ŽP for the gut microbiota of chickens

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