Study of IgG Subclass Profiles of Anti-HBs in Populations With Different HBV Infection Status

Tsai, Tzu-Hsin; Huang, Chun-Ming; Wei, James Cheng‐Chung; Ho, Mei‐Shang; Wang, Lina; Tsai, Wei-Yu; Lin, Chien-Chou; Xu, Fangling; Yang, Chi-Chiang

doi:10.1089/vim.2006.19.277

Cited by 9 publications

(13 citation statements)

References 24 publications

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“…The IgG subclass pattern demonstrated in chronic carriers was consistent with the findings of Sä llberg et al [1990]. Moreover, the anti-HBe IgG2 level was the same as that demonstrated in earlier anti-HBc [Yang et al, 2001 and anti-HBs Tsai et al, 2006] studies by this group, and it was the lowest IgG isotype in chronic carriers and recovered individuals of both genders. The IgG subclass profiles of anti-HBe in chronic carriers did not change with liver inflammation (elevated ALT and AST) and were independent of sex and age, although the mean OD for the patients with elevated ALT and AST was higher.…”

Section: Discussionsupporting

confidence: 90%

“…Earlier studies suggested that IgG1 is the most predominant anti-HBs IgG subclass in chronic carrier, recovered, and vaccinated groups . Samples from both chronic carriers and vaccinees exhibited a significantly higher concentration of antiHBs total IgG and IgG1 than those from recovered patients [Tsai et al, 2006]. The results of previous studies also suggest that, for asymptomatic carriers, the predominant anti-HBc subclass is IgG1, while IgG3 titers are higher relative to IgG1 in patients with liver cirrhosis [Akbar et al, 1991;Yang et al, 2001Yang et al, , 2005.…”

Section: Introductionmentioning

confidence: 89%

“…The data were then used to select test plates for further analysis of the anti-HBe IgG subclass patterns in 543 samples at different stages of infection with HBV. The anti-HBe IgG subclasses were compared with analogous data from previous reports of both anti-HBc [Yang et al, 2001 and anti-HBs IgG subclasses Tsai et al, 2006] in different groups. This data may elucidate the relationship between these patterns and the immune response induced by HBV antigenic proteins.…”

Section: Introductionmentioning

confidence: 96%

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Utilizing self‐prepared ELISA plates for a cross‐population study of different anti‐HBe IgG subclass profiles

Wang

Tsai

Huang

et al. 2007

Journal of Medical Virology

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Fourteen serum samples obtained from hepatitis B virus (HBV) chronic carriers and patients recovered from hepatitis B infection were used with four sodium dodecyl sulfate-treated enzyme-linked immunosorbent assay (ELISA) plates available commercially, and one self-prepared HBcAg analog for evaluation of anti-HBe subclass pattern absorbance. The self-prepared plates had the best performance and were thus used for samples obtained from 104 (60 male and 44 female) HBV chronic carriers and 439 (247 male and 192 female) recovered individuals. Tests for aspartate aminotransferase (AST) and alanine aminotransferase (ALT) were also carried out in 21 of the subjects (>25 IU/ml). Statistical comparison of these patients with elevated ALT/AST levels with other ALT/AST-normal chronic carriers revealed no significant differences in the anti-HBe OD, although the mean optical density (OD) of patients with elevated ALT/AST levels was higher. The results suggest that the anti-HBe IgG subclass profiles in the chronic carriers did not change with inflammation of the liver, and were independent of sex and age. In contrast to previous anti-HBc findings, the distribution pattern of anti-HBe subclasses in HBV chronic carriers was IgG1 > IgG4 > IgG3 while in the recovered individuals it was IgG1 > IgG3 > IgG4, for both males and females. Subclasses IgG1 and IgG2 were the most and least prevalent isotypes, respectively, in both study groups. The results of the study suggest that induction of IgG1 and/or IgG3 antibodies is important for effective virus neutralization, while IgG2 antibodies are of limited importance. Significantly higher OD values for anti-HBe IgG4 were observed when comparing samples from the chronic carriers and recovered individuals, which may reflect the effects of persistence. Further, in contrast to previous anti-HBs results, the concentrations of total IgG and IgG1 were higher in the samples from chronic carriers relative to those from recovered individuals.

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Section: Discussionsupporting

confidence: 90%

Section: Introductionmentioning

confidence: 89%

Section: Introductionmentioning

confidence: 96%

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Utilizing self‐prepared ELISA plates for a cross‐population study of different anti‐HBe IgG subclass profiles

Wang

Tsai

Huang

et al. 2007

Journal of Medical Virology

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“…However, we believe that this also holds true for other gastroenteroviruses, since our previous studies and other research groups have shown that IgG1 and IgG3 are the main IgG subclasses found in infection by human cytomegalovirus [81], human herpes virus 6 [82], hantavirus [83,84], and human immunodeficiency virus [85], and hepatitis B virus [86][87][88][89]. The cross-regulation between Th1 and Th2 cytokines is associated with the development of antigen pathogenesis [64].…”

Section: Transmissions Of Gastroenteroviruses and Host Immune Responsesmentioning

confidence: 77%

Gastroenteroviruses Infection in Taiwan

Tsai

Cheng³

et al. 2009

TOIDJ

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Acute infectious gastroenteritis causes high morbidity and mortality in children worldwide. The most important cause of gastroenteritis is virus infection. Diarrhea is the dominant symptom of viral gastroenteritis. It subsequently induces dehydration and malnutrition, which contribute to high morbidity and mortality in children. In Taiwan, rotavirus is the major cause (30.4-48%) of infectious gastroenteritis, followed by adenovirus (9.1-19.8%), norovirus (8.2-25%), enterovirus (< 5.2 %), astrovirus (2.7-2.9%) and other viruses (including sapovirus)(< 1 %). Untreated gastroenteritis is the major cause of infantile mortality, which leads to human tragedy and economic burden. In Taiwan, the cost of admission or hospitalization associated with gastroenterovirus infection is about more than 40% of the monthly salary of an unskilled or service worker. Understanding the epidemiology of gastroenterovirus and its induced host immune response could benefit the development for prevention and therapeutic management of gastroenterovirus. This review summarizes the epidemiological information and current knowledge of gastroenteroviruses that cause gastroenteritis in Taiwan.

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“…In BALB/c mice, IgG1 was the predominant subclass response when the orally administered subunit vaccines were delivered with CT as an adjuvant (12). IgG1 has also been reported to be the predominant IgG subclass of anti-HBs (2,14,17).…”

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confidence: 99%

Antibody Responses in Mice Stimulated by Various Doses of the Potato-Derived Major Surface Antigen of Hepatitis B Virus

Youm

Won

Jeon

et al. 2010

Clin Vaccine Immunol

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The ability of potato-derived major surface antigen of hepatitis B virus (P-HBsAg) to elicit antibody responses to different dosages of P-HBsAg ranging from 0.02 to 30 g administered orally in mice was examined. All immunized groups produced specific serum IgG and fecal IgA antibodies against P-HBsAg, even at low levels (<5 g), after administration of a 0.5-g yeast-derived HBsAg (Y-HBsAg; LG Life Sciences, Republic of Korea) booster.The major surface antigen of hepatitis B virus (HBV) (HBsAg) is one of the best-developed examples of a plant-derived antigen (6,9,11,13,18); however, low expression levels prevent plant-derived antigens from being economically competitive (5,15,16). Average antigen expression levels are in the range of 0.01% to 0.4% of total soluble protein (TSP). Aspects related to eliciting protective antibody responses, e.g., oral administration doses (1, 10), efficient delivery of oral vaccines (3, 4), and functional effects of adjuvants (12), were identified to stimulate immune responses after vaccination with low levels of plant-derived antigen.The objective here was to examine the effects of plant-based oral immunization on HBV-specific immune responses over a broad range of doses, from the lowest dose of 0.02 g potatoderived HBsAg (P-HBsAg) to the maximum dose of 30 g P-HBsAg. IgG humoral and IgA mucosal responses were observed at various P-HBsAg doses, and these results are discussed in relation to the optimization of plant-derived vaccines. Furthermore, the analysis of IgG subclass distribution following oral administration with varied doses of P-HBsAg was carried out to understand the mechanism of the immune response.To evaluate the immunogenicity upon oral administration of various doses of P-HBsAg from a plant line showing the highest production of HBsAg (7, 18), mice were immunized orally with tuber extract on days 1, 7, and 14. The detailed procedure was described previously (18). Mice were immunized with 150 g of yeast-derived HBsAg (Y-HBsAg; LG Life Sciences, Republic of Korea) mixed with 10 g of Cholera toxin (CT; Sigma) as a positive control. Each concentration of P-HBsAg administered to mice was divided into one of the the following three groups: lower level, consisting of 0.02, 0.1, and 0.5 g; middle level, consisting of 1.0, 2.5, and 5.0 g; and higher level, consisting of 10 g, 15 g, and 30 g. Only five concentrations (0.1, 1.0, 5.0, 10, and 30 g) were shown among the nine concentrations depicted in the figures to help with understanding by using a concise arrangement. Antigen-specific IgG responses to representative doses in mouse sera against P-HBsAg were graphically monitored up to week 12 (Fig. 1a). The groups administered 10 g and 30 g of antigen stimulated slight primary responses of 41 mIU and 51 mIU, respectively, at 7 weeks before booster administration compared to responses of the mice immunized with potato extract from the nontransformed (NT) plant (NT group) (4 mIU). Mice administered higher levels of P-HBsAg (10, 15, and 30 g) exhibited significantly increased immu...

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Study of IgG Subclass Profiles of Anti-HBs in Populations With Different HBV Infection Status

Cited by 9 publications

References 24 publications

Utilizing self‐prepared ELISA plates for a cross‐population study of different anti‐HBe IgG subclass profiles

Utilizing self‐prepared ELISA plates for a cross‐population study of different anti‐HBe IgG subclass profiles

Gastroenteroviruses Infection in Taiwan

Antibody Responses in Mice Stimulated by Various Doses of the Potato-Derived Major Surface Antigen of Hepatitis B Virus

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