Study of In-Vitro Antiviral Activity of Aminoglycosides on Foot and Mouth Disease Virus

Abstract

Background: The aminoglycosides family of antibiotics are widely used as a prophylactic measure against bacterial contamination in animal vaccine production. Objective: Screening the antiviral activity of some members of aminoglycosides on foot and mouth disease (FMD) viruses used in the preparation of FMD vaccine in veterinary serum and vaccine research institute (VSVRI). Method: This study was conducted by in-vitro assessment of cytopathic effect (CPE) and infectivity titers of FMD viruses on Baby hamster kidney (BHK) cells under different concentrations of Neomycin (200, 500 mg/L), kanamycin (200, 500 mg/L), gentamycin (200, 500 mg/L) and streptomycin (200, 500 mg/L) as members of aminoglycosides using the inverted microscope. Positive (aminoglycosides-free) and negative (FMD viruses-free) were used. Results: Our results show that aminoglycosides antibiotics could inhibit the cytopathic effect and reduce the infectivity titers of FMD viruses. Conclusion: Finally we recommend the avoidance of routine inclusion of aminoglycosides antibiotics in tissue culture technique or treating FMD virus suspensions.