Study of interaction between Papaya ringspot virus coat protein and infected Carica papaya proteins

Abstract

Papaya ringspot virus (PRSV) causes the Papaya ringspot disease. Virus-host interactions appear to play a significant role in the replication, pathogenesis, and infection caused by the PRSV. PRSV coat protein is likely to be involved in the processes of RNA replication, aphid transmission, and cell-to-cell movement, which is closely related to the host cell proteins resulting in a PRSV infection. To identify the host proteins that interact with the CP in vitro, immune precipitation, insolution trypsin digestion, and LC-MS/MS were performed. Twenty-three identified proteins that interacted with the CP are involved in cellular metabolism, transcription, signal, translation, carbohydrate metabolism, protein metabolism, stress response, photosynthesis, nucleotide metabolism, respiration, and lipid metabolism processes. The search tool for interactions of chemicals (STITCH) results show that SWP (an RNA polymerase II transcription mediator) and MPPBETA (Mitochondrial processing peptidase) are involved in known plant defense mechanisms including transcription factors, cell division, hormones, stress, mitochondrial electron transferase, respiration, and proteasome. Therefore, analyzing virus-host protein interactions at a molecular level is important to build a better understanding of the virus replication mechanism and cellular responses mounted against viruses by the host defense system.

In-silico comparative modeling and interaction studies of PRSV proteins - Accelerated towards dissection of structure based evolutionary divergence and functional interaction of virus within the host

“…Coat protein of RNA viruses holds many other activities a part from their well-recognized structural activity [57]. photosynthesis, nucleotide metabolism, respiration and lipid metabolism [58]. The interaction values in our study has provided the probable functions of these proteins are within the host further suggested whether the interaction between host and virus though these proteins is compatible or not.…”

“…Previously, in vitro approaches that determined the interaction of CP inside papaya identified, twenty-three host proteins interacted with invaded viral CP. Those proteins were mainly involved in cellular metabolism, transcription, translation, carbohydrate metabolism, protein metabolism, stress response, photosynthesis, nucleotide metabolism, respiration and lipid metabolism [58]. The interaction values in our study has provided the probable functions of these proteins are within the host further suggested whether the interaction between host and virus though these proteins is compatible or not.…”

Tropical Fruit Virus Resistance in the Era of Next Generation Plant Breeding

“…Papaya ringspot virus papaya-strain (PRSV-p, a potyvirus) is the etiological agent of the most devastating disease of papaya crop, named ringspot/mosaic disease, since the symptoms constitute the appearance of a mosaic in leaf tissues, and a yellow-circular spot on the fruits [40,41]. During potyviruses infection, the host defense response and innate immune system are activated [42], and specifically to PRSV, a protein interaction between viral coat protein (CP) and papaya identified 23 proteins grouped in four groups, as: transcription factors and cell division; hormones and stress; mitochondrial electron transferase and respiration; and proteasome [43].…”

Comparative RNA-seq analysis of resistant and susceptible banana genotypes reveals molecular mechanisms in response toBanana bunchy top virus(BBTV)

“…Increased nucleotide metabolism is a metabolic change linked to a variety of viral infections 89,90 . Previous study demonstrates the interaction between host proteins involved in nucleotide biosynthesis and Papaya ringspot virus (PRSV) coat protein (CP) during infection in papaya 91 , signifying the potential role of these genes in disease progression in susceptible hosts. Virulent pathogens must overcome the initial layer of resistance of the host for successful infection, leading to disease development.…”