Study of interaction of proteins with fumed silica in aqueous suspensions by adsorption and photon correlation spectroscopy methods

Immobilization of Candida antarctica Lipase B on fumed silica

“…According to Gun'ko et al 32 , the adsorption of proteins (e.g., Bovine Serum Albumin) on fumed silica in aqueous suspensions has many intricacies and depends on the morphology of the fumed silica, the protein type and concentration, pH, ionic strength, and fumed silica concentration, among other parameters. To form the preparations at the various wt% of fumed silica shown here, a rapidly increasing amount of fumed silica was required (Table 1).…”

“…Fumed silica possesses a large specific surface area and exhibits exceptional adsorptive affinity for various organic molecules in aqueous solution including proteins and polymers [26][27][28][29][30][31] . The adsorption of proteins onto fumed silica has been reported to be essentially irreversible 29,32 . Adsorption is promoted by the formation of non-covalent interactions between the available groups on the surface of the fumed silica and the carbonyl or amino groups present in the enzyme molecules 29,32 .…”

“…The adsorption of proteins onto fumed silica has been reported to be essentially irreversible 29,32 . Adsorption is promoted by the formation of non-covalent interactions between the available groups on the surface of the fumed silica and the carbonyl or amino groups present in the enzyme molecules 29,32 . In general, these interactions are electrostatic in nature and driven by the net charges of the protein and the surface, therefore, they can be repulsive or attractive 33,34 .…”

Adsorption of organic molecules on silica surface

“…The interaction of fumed silica A-300 (S BET = 297 m 2 g − 1 ) with bovine serum albumin (prepared by different methods), ovalbumin, human hemoglobin, and gelatin in aqueous medium was studied using photon correlation spectroscopy (PCS) methods [308]. Comparison of equilibrium adsorption of proteins on A-300 (with approximately 1 h of incubation time), minute flocculation rate (approximately 1 min) and the particle size distributions measured by the PCS method shows different rearrangement of particle swarms depending on pH, salinity, and concentration of proteins, especially at pH close to isoelectric potential (IEP) of silica or proteins.…”

Impedance analysis of an electrode-separated piezoelectric sensor as a surface-monitoring technique for gelatin adsorption on quartz surface

“…The adsorption behavior of gelatin is expected to complex. Some recent experimental investigations of gelatin adsorption onto mica [38], phosphatidyl choline-coated silica [39], fumed silicas [40,41], carbon particles [42], and polystyrene latices [43] have been reported. Several experimental methods have been employed for studying gelatin adsorption, including solution depletion [44,45], ellipsometry [46], surface force apparatus [47,48], and small-angle neutron scattering [49].…”