Study of intracellular ion composition of yeast Saccharomyces cerevisiae biomass

Оверченко, М.Б.; Игнатова, Н. И.; Shelekhova, N.V.; Серба, Елена Михайловна; Krivova, А. Yu.

doi:10.3103/s106836741702015x

Cited by 4 publications

(3 citation statements)

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“…Although the highest activity of YLR446Wp was observed in the presence of Co 2+ , only a trace amount of intracellular Co 2+ was detected 14 . On the other hand, Mg 2+ is abundant in yeast cells and is known as a major co-factor for ATP-dependent kinases 15 . Therefore, we decided to carry out the enzyme assay in the presence of Mg 2+ in the subsequent experiments.…”

Section: Resultsmentioning

confidence: 99%

Identification and biochemical characterization of a novel N-acetylglucosamine kinase in Saccharomyces cerevisiae

Umekawa

Nishikawa

Isono

et al. 2022

Sci Rep

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N-acetylglucosamine (GlcNAc) is a key component of glycans such as glycoprotein and the cell wall. GlcNAc kinase is an enzyme that transfers a phosphate onto GlcNAc to generate GlcNAc-6-phosphate, which can be a precursor for glycan synthesis. GlcNAc kinases have been found in a broad range of organisms, including pathogenic yeast, human and bacteria. However, this enzyme has never been discovered in Saccharomyces cerevisiae, a eukaryotic model. In this study, the first GlcNAc kinase from S. cerevisiae was identified and named Ngk1. The Km values of Ngk1 for GlcNAc and glucose were 0.11 mM and 71 mM, respectively, suggesting that Ngk1 possesses a high affinity for GlcNAc, unlike hexokinases. Ngk1 showed the GlcNAc phosphorylation activity with various nucleoside triphosphates, namely ATP, CTP, GTP, ITP, and UTP, as phosphoryl donors. Ngk1 is phylogenetically distant from known enzymes, as the amino acid sequence identity with others is only about 20% or less. The physiological role of Ngk1 in S. cerevisiae is also discussed.

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Section: Resultsmentioning

confidence: 99%

Identification and biochemical characterization of a novel N-acetylglucosamine kinase in Saccharomyces cerevisiae

Umekawa

Nishikawa

Isono

et al. 2022

Sci Rep

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“…We purified ScTop2, N-terminally labeled the protein with a Cy3 fluorophore, buffer exchanged the sample into high salt (600 mM), and diluted the labeled protein to physiological salt concentrations (150 mM) to induce phase separation. Potassium acetate (KOAc) was chosen as a salt for these and other subsequent experiments, as K + is the predominant monovalent cation and acetate is a high abundance anion in both human and yeast cells (internal Na + and Cl - concentrations are negligible in these systems) (Ince et al, 1987; Rimareva et al, 2017). After dilution, samples were imaged using fluorescence confocal microscopy ( Figure 2C ).…”

Section: Resultsmentioning

confidence: 99%

DNA-Stimulated Liquid-Liquid Phase Separation by Eukaryotic Topoisomerase II Modulates Catalytic Function

Jeong

Lee

Carcamo

et al. 2022

Preprint

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Type II topoisomerases modulate chromosome supercoiling, condensation, and catenation by moving one double-stranded DNA segment through a transient break in a second duplex. How DNA strands are chosen and selectively passed to yield appropriate topological outcomes (e.g., decatenation vs. catenation) is poorly understood. Here we show that at physiological enzyme concentrations, eukaryotic type IIA topoisomerases (topo IIs) readily coalesce into condensed bodies. DNA stimulates condensation and fluidizes these assemblies to impart liquid-like behavior. Condensation induces both budding yeast and human topo IIs to switch from DNA unlinking to active DNA catenation, and depends on an unstructured C-terminal region, the loss of which leads to high levels of knotting and reduced catenation. Our findings establish that local protein concentration and phase separation can regulate how topo II creates or dissolves DNA links, behaviors that can account for the varied roles of the enzyme in supporting transcription, replication, and chromosome compaction.

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“…Качество пробиотиков определяется совокупностью следующих критериев: устойчивостью отобранных штаммов микроорганизмов к неблагоприятным факторам среды; кислотообразующей активностью; способностью продуцировать витамины, белки и жирные кислоты; антагонистической активностью по отношению к патогенным микроорганизмам и другими факторами [9][10][11]. По данным профессора Новосибирского ГАУ Ноздрина Г.А., ведущего специалиста в области ветеринарной фармакологии пробиотических препаратов, влияние пробиотиков на микроэкологию пищеварительного тракта выражается в оптимизации микробиоценоза кишечника, стимуляции иммунной системы, а также в повышении интенсивности процессов пищеварения, роста и развития животных [12].…”

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Use of probiotic preparations to increase productive qualities in goats: a review

Yashkin,

Afanasyeva

2023

Vestn. Novosib. gos. agrar. univ.

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An analytical review of scientific publications on probiotics in goat breeding was carried out. It has been established that the most popular probiotic microorganisms in goat farming are bacteria of the species Bacillus subtilis, Bifidobacterium bifidum, Enterococcus faecium, Lactobacillus acidophilus, Lactobacillus plantarum and single-celled yeast of the species Saccharomyces cerevisiae. Including probiotic microflora in the diet increases the number of bacteria in the rumen contents of goats. Reducing the number of Clostridia and Escherichia coli in animal faeces is achieved by replacing the native faecal microflora with streptococci and lactic acid bacteria. It has been shown that feeding probiotic preparations is accompanied by an improvement in the metabolic status in the body of goats and is expressed by an increase in the concentration of total protein and its fractions, haemoglobin, erythrocytes, macro- and microelements in the blood of animals, and optimisation of lipid metabolism. Probiotics help preserve villi and inhibit inflammation of the intestinal epithelium of animals, exhibit antimutagenic and anticarcinogenic properties, and help reduce putrescine concentration in faeces. Feeding probiotics ensures an increase in daily milk yield, fat and protein content by increasing the digestibility of diet solids, primarily crude fibre. The probiotic potential of some microorganisms is expressed in increased concentrations of unsaturated fatty acids in goat milk and meat with a decrease in the atherogenic index of raw materials. The use of drugs helps to increase the absolute increase in live weight of goat kids against the background of the harmonious development of the physique of young animals due to the production of microbial phytase, a decrease in the redox potential of chyme and an increase in the number of microorganisms that consume lactic acid. Some authors report the absence of a significant effect when using probiotics in feeding lactating animals and raising goat kids.

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Study of intracellular ion composition of yeast Saccharomyces cerevisiae biomass

Cited by 4 publications

References 1 publication

Identification and biochemical characterization of a novel N-acetylglucosamine kinase in Saccharomyces cerevisiae

Identification and biochemical characterization of a novel N-acetylglucosamine kinase in Saccharomyces cerevisiae

DNA-Stimulated Liquid-Liquid Phase Separation by Eukaryotic Topoisomerase II Modulates Catalytic Function

Use of probiotic preparations to increase productive qualities in goats: a review

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