Background: Staphylococcus aureus is a gram-positive spherical bacterium. It is a human skin and respiratory flora which is considered as one of the most common cause of burn infections. It always finds a way to resist antibiotics due to that it had virulence and resistance genes as well as acquired of new genes from other strains which makes it more dangerous. Aims: This study aims at investigating the prevalence of genetic evaluations for Staphylococcus aureus in the burn unit. Methods: A total of 223 burn swabs were collected from the burn patient's unit during the period from September to December 2019 in AL-Hussain Teaching Hospital in AL-Nasiriya City, Southern of Iraq. These 223 burn swabs were subjected to macroscopic, microscopic, cultures, biochemical test and out of the 223 clinical swab only (70) undergoes the PCR and DNA sequence technique looking for new emergence strains. Results: The current study used partial 16SrRNA gene sequences for (6) Staphylococcus aureus isolates and found (5) of them were global and one was local according to the accession numbers of NCBI Gene bank, MT605440.