Study of Lipase Production by Acinetobacter sp. YMP

Abstract

The biosurfactant producing Acinetobacter sp. YMP which showed vegetable oil degradation potential on MSM was studied for lipase production. The production of lipase was confirmed on fat agar & lipase activity was measured by titrimetric method. The effect of environmental factors (agitation, time span , substrate concentration , pH & temperature) on lipase production was studied by using Acinetobacter sp. YMP. The enzyme was further purified by Ammonium sulphate (60%) precipitation technique. The maximum lipase production by Acinetobacter sp. YMP was found to be within 120 hours at 450 rpm, 3.0 pH , 27° C temperature & at 2% substrate concentration. This species was also able to show upto 8 % oil degradation potential on MSM broth & agar.