Study of Membrane Protein Monolayers Using Surface-Enhanced Infrared Absorption Spectroscopy (SEIRAS): Critical Dependence of Nanostructured Gold Surface Morphology

Seica, Ana Filipa Santos; Iqbal, Muhammad Haseeb; Carvalho, Alain; Choe, Juhui; Boulmedais, Fouzia; Hellwig, Petra

doi:10.1021/acssensors.1c00238

Cited by 12 publications

(12 citation statements)

References 51 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Infrared spectroscopy analysis is one of the common methods used to resolve the structure, and each group has a specific infrared absorption peak [ 19 ]. We used infrared spectrometer to determine the infrared spectrum of IHFM and found that soluble IHFM had a strong and broad characteristic absorption at 3313.39 cm −1 , corresponding to the –NH group attached to the –OH group in the indole ring.…”

Section: Resultsmentioning

confidence: 99%

Optimization of Solid-State Fermentation Extraction of Inonotus hispidus Fruiting Body Melanin

Zhang

Xue

et al. 2021

Foods

View full text Add to dashboard Cite

Melanin has good nutritional and medicinal value; however, its extraction rate is extremely low. This study explored the edible and medicinal fungus Inonotus hispidus fruiting body melanin (IHFM) extraction process and solid-state fermentation conditions. The results showed that the best way to extract IHFM is the compound enzymatic method, with complex enzyme 26.63 mg/g, liquid material ratio 5:1, enzymatic hydrolysis 80 min, pH 4.61, and enzymolysis temperature at 36.07 °C. The yield of IHFM was 23.73 ± 0.57%, which was equivalent to 1.27 times before optimization. The best solid medium formula was normal pH, rice 20 g per cultivation bottle, maltose 22 g/L, beef extract 4.4 g/L, carbon-nitrogen ratio 5:1, and liquid-to-material ratio 1.1:1, where the IHFM yield was 31.80 ± 1.34%, which was equivalent to 1.7 times that before optimization. In summary, solid-state fermentation and extraction optimization greatly improved the yield of melanin, provided a reference to produce melanin, and laid a foundation for the development and utilization of melanin.

show abstract

Section: Resultsmentioning

confidence: 99%

Optimization of Solid-State Fermentation Extraction of Inonotus hispidus Fruiting Body Melanin

Zhang

Xue

et al. 2021

Foods

View full text Add to dashboard Cite

show abstract

“…A three-dimensional network of gold nanoparticles (AuNPs) was formed on the surface of Si substrate as previously described [ 47 ]. First the silicon substrate was polished with fine grade 0.3 μm alumina, rinsed with distilled water, acetone, and again water.…”

Section: Methodsmentioning

confidence: 99%

Plasmonic Resonant Nanoantennas Induce Changes in the Shape and the Intensity of Infrared Spectra of Phospholipids

et al. 2021

Self Cite

View full text Add to dashboard Cite

Surface enhanced infrared absorption spectroscopic studies (SEIRAS) as a technique to study biological molecules in extremely low concentrations is greatly evolving. In order to use the technique for identification of the structure and interactions of such biological molecules, it is necessary to identify the effects of the plasmonic electric-field enhancement on the spectral signature. In this study the spectral properties of 1,2-Dipalmitoyl-sn-glycero-3 phosphothioethanol (DPPTE) phospholipid immobilized on gold nanoantennas, specifically designed to enhance the vibrational fingerprints of lipid molecules were studied. An AFM study demonstrates an organization of the DPPTE phospholipid in bilayers on the nanoantenna structure. The spectral data were compared to SEIRAS active gold surfaces based on nanoparticles, plain gold and plain substrate (Si) for different temperatures. The shape of the infrared signals, the peak positions and their relative intensities were found to be sensitive to the type of surface and the presence of an enhancement. The strongest shifts in position and intensity were seen for the nanoantennas, and a smaller effect was seen for the DPPTE immobilized on gold nanoparticles. This information is crucial for interpretation of data obtained for biological molecules measured on such structures, for future application in nanodevices for biologically or medically relevant samples.

show abstract

“…Apart from the gold cluster size, which is governed by the evaporation time, also the immobilization procedure used for binding the proteins to the plasmonic surface affected the EF of the protein monolayer. 29…”

Section: Biological Sensingmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

“…23,24 Substrates may be designed to enhance specific molecular vibrations in a mixture (for example proteins, fatty acids or environmental toxins), leading to the selective enhancement of a compound over others. 20,[25][26][27][28][29] Furthermore, since SEIRA solely probes the sample close to the surface, the IR water absorption peak can be suppressed. 5 Recent advances in pattern design of metallic SEIRA substrates have seen the emergence of multi-resonant substrates, which can enhance multiple sections of the IR range, thereby allowing for the detection of multiple analytes.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Towards multi-molecular surface-enhanced infrared absorption using metal plasmonics

2022

View full text Add to dashboard Cite

show abstract

Study of Membrane Protein Monolayers Using Surface-Enhanced Infrared Absorption Spectroscopy (SEIRAS): Critical Dependence of Nanostructured Gold Surface Morphology

Cited by 12 publications

References 51 publications

Optimization of Solid-State Fermentation Extraction of Inonotus hispidus Fruiting Body Melanin

Optimization of Solid-State Fermentation Extraction of Inonotus hispidus Fruiting Body Melanin

Plasmonic Resonant Nanoantennas Induce Changes in the Shape and the Intensity of Infrared Spectra of Phospholipids

Towards multi-molecular surface-enhanced infrared absorption using metal plasmonics

Contact Info

Product

Resources

About