Study of miR-143 expression in stomach cancer

Abstract

The present study was planned to investigate miR-143 expression during stomach cancer. The study explored the relationship between miR-143 expression and clinicopathological characteristics including proliferation, migration and apoptosis of stomach cancer cells. Sixty-three samples from each of stomach cancer tissue and surrounding tissue were obtained. Total RNA was extracted. The expression levels of miR-143 from stomach cancer tissue as well as from surrounding tissue were measured by semi-quantitative PCR. The effects of miR-143 overexpression on the migration of stomach cancer cells were examined by Transwell assay. The effects of miR-143 overexpression on the apoptosis of stomach cancer cells were examined by flow cytometer. The expression level of miR-143 was significantly decreased in stomach cancer tissues in comparison to surrounding tissues (P<0.01). Moreover, the expression of miR-143 related well with the tumor size, TNM stage, lymphatic metastasis and relapse (P<0.01). On the other hand, stomach cancer cell line with overexpression of miR-143, showed significant decline in proliferation rate and migration rate comparison to control cells (P<0.01). However, it showed significant higher in apoptosis rate (P<0.01). The present study concluded that expression of miR-143 is low during stomach cancer. Further, higher expression levels of miR-143 have the ability to decline proliferation and migration of stomach cancer cells. In this manner, the expression level of miR-143 could be used as an important factor to determine the severity of stomach cancer.

<p>miR-30a-3p Targets MAD2L1 and Regulates Proliferation of Gastric Cancer Cells</p>

“…Moreover, it has been shown that miR-30a-3p can exert anti-cancer effects by targeting on both COX-2 and BCL9, which significantly affects the development of gastric cancer induced by Helicobacter pylori, providing new insights to better understand the mechanism underlying the disease development 45. Similarly, miR-944,46 miR-139,47 miR-511b48 and miR-143-3p49,50 have also been shown to be the inhibiting factors of the malignant behavior of gastric cancer. It has been also shown that MAD2L1 exerts function as a cancer-promoting factor in the gastric cancer.…”