Study of Monocyte Subsets and Their Surface Expression of CD86 and Serum IL-17 Compared to Serum Procalcitonin as Markers of Early Neonatal Sepsis

Sehmawy, Asmaa A El; Abdul-Mohymen, Abeer Mohammed; Seliem, Nora; Elamir, Reham Y; Ibrahim, Hanan F; Mahmoud, Nihal A; Abdou, Aml E

doi:10.2147/idr.s335057

Cited by 7 publications

(4 citation statements)

References 29 publications

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“…When examining the remaining CD86 and TIM-3 markers related to the activation or depletion of monocytes, we did not find significant differences between the groups. El Sehmawy et al [51] show that healthy people have lower percentages of monocytes expressing CD86 compared to the COVID-19 patients in our study.…”

Section: Discussionsupporting

confidence: 50%

Intermediate Monocytes with PD-L1 and CD62L Expression as a Possible Player in Active SARS-CoV-2 Infection

Rutkowska

Kwiecień

Kłos

et al. 2022

Viruses

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Monocytes play a role in viral biology, but little is known about the monocyte subpopulation in the course of COVID-19 disease. The aim of the study was the analysis of classical, intermediate and non-classical monocytes with expression of PD-L1 and CD62L, TIM-3 and CD86 molecules in peripheral blood (PB) to distinguish patients with SARS-CoV-2 infection from convalescent patients. The study group consisted of 55 patients with SARS-CoV-2 infection and 51 convalescent patients. The cells were analyzed by flow cytometry. The number and proportion of monocytes were lower in patients with COVID-19 than convalescent patients. We observed a lower proportion of non-classical monocytes in COVID-19 patients than convalescent ones. There was a higher proportion of PDL-1-positive intermediate monocytes in COVID-19 patients than convalescent ones. We noticed a higher geometric mean fluorescence intensity (GeoMean) of PD-L1 on intermediate monocytes in COVID-19 patients than convalescent patients, and a higher proportion of CD62L-positive monocytes in COVID-19 patients in comparison with convalescent ones. We found a higher GeoMean of CD62L on monocytes in COVID-19 patients than convalescent ones. Assessment of PD-L1- and CD62L-positive monocyte subsets may identify patients with a possible predisposition for rapid recovery. The monitoring of monocyte subsets in PB might be a useful test in COVID-19 patients.

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Section: Discussionsupporting

confidence: 50%

Intermediate Monocytes with PD-L1 and CD62L Expression as a Possible Player in Active SARS-CoV-2 Infection

Rutkowska

Kwiecień

Kłos

et al. 2022

Viruses

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“…Here, using an in vivo SCI model, we showed that the percentage and MFI of BMP4 + circulating monocytes at dpi-3 were markedly higher in SCI rats than in sham rats, indicating that monocytes are an important candidate for the BMP4 upregulation in the circulation. We evaluated CD86 as a marker of activated monocytes based on previous research in which CD86 + monocytes displayed strong capability to present antigen ( Ugurel et al, 2004 ) and initiate lymphocyte activation ( Pinto et al, 2018 ), and were highly correlated with higher levels of inflammatory cytokines ( Nowak et al, 2019 ; El Sehmawy et al, 2021 ). The results showed that BMP4 is more abundantly expressed in the CD86 + monocytes.…”

Section: Discussionmentioning

confidence: 99%

Infiltrating circulating monocytes provide an important source of BMP4 at the early stage of spinal cord injury

Shen

Liu

Hao

et al. 2023

Disease Models &Amp; Mechanisms

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Background Bone morphogenetic protein-4 (BMP4) plays a critical role in regulating neuronal and glial activity in the course of spinal cord injury (SCI). However, the underlying cause and cellular source of the BMP4 accumulation at the injured spinal cord remains unclear. Method Firstly, the SCI patients and healthy donors were enrolled to test their plasma concentrations of BMP2/4/7 and the antagonist Noggin using enzyme linked immunosorbent assay (ELISA). Secondly, rats were randomly divided into 3 groups: the Sham group (T9 laminectomy without SCI), the SCI group and the SCI+CLL group (circulating monocytes depletion before SCI). For each group, Basso-Beattie-Bresnahan (BBB) scales and immuno-histochemistry were employed to evaluate the functional and histological changes; ELISA was conducted to test the expression patterns of BMP4, Noggin and neurofilament light polypeptide (NEFL) both in blood and cerebrospinal fluid (CSF); western blotting and flow cytometry were further performed to investigate the BMP4 expressions in the spinal cord, and particularly in the circulating monocytes and infiltrating monocyte-derived macrophages (MDMs) respectively. Results Firstly, the level of BMP4, instead of BMP2/7 was statistically higher in the SCI patients than in the healthy donors. Secondly, compared with the Sham group, rats in the SCI group developed a persistent decline in the BBB scores, together with evident necrosis and the accumulation of mononuclear cells at the contusion site. Additionally, both plasma and CSF levels of BMP4, Noggin and NEFL displayed notable elevations throughout two weeks after SCI, and positive correlations could be found between blood and CSF in all indicators above. Importantly, BMP4 expression displayed upregulation in the injured spinal cord, and the percentage of BMP4 positive circulating monocytes and infiltrating MDMs were both higher in the SCI group than in the Sham group. Finally, in the SCI+CLL group, depletion of circulating monocytes effectively relieved BMP4 accumulation in the injured spinal cord. Conclusion Following SCI, infiltrating MDMs provide an important source of BMP4 in the injured spinal cord. Depletion of circulating monocytes effectively downregulates BMP4 levels in the spinal cord at the early stage of SCI, which might serve as a potential therapeutic target.

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“…Asmaa et al.’s study of 30 neonatal sepsis (a total of 60 participates) showed that compared with the control group, CD86 + monocytes in the sepsis group were significantly higher (median 78.4% vs. 24.0%, P < 0.001). When the cutoff value was 36%, the AUROC for early diagnosis of neonatal sepsis was as high as 0.951, and the sensitivity and specificity were 90% ( 26 ). Céspedes et al.’s malaria-associated bacteremia in a rodent model demonstrated a significantly high expression of mast cell-activating cytokines (IL-9 and IL-13).…”

Section: Discussionmentioning

confidence: 99%

Diagnostic and Predictive Values of Ferroptosis-Related Genes in Child Sepsis

Zhang²,

Liu³

et al. 2022

Front. Immunol.

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BackgroundEarly diagnosis of sepsis in children was essential to reducing mortality. This study aimed to explore the value of ferroptosis-related genes in children with sepsis.MethodsWe screened the septic children microarray dataset from the GEO database and analyzed the ferroptosis-related differentially expressed genes (DEGs). A functional analysis of ferroptosis-related DEGs was performed. The protein–protein interaction network was used to identify hub genes. We explored the immune landscape of sepsis and controls. The value of hub genes in diagnosing sepsis was tested in the training (GSE26440) and validation sets (GSE13904), and ELISA was used to verify their diagnostic value in children with sepsis in our hospital.ResultsA total of 2,103 DEGs in GSE26440 were obtained, of which ferroptosis-related DEGs were 34. Enrichment analysis showed significant enrichment in the ferroptosis and hypoxia pathways (i.e., HIF-1 pathway). The top three genes (HMOX1, MAPK14, TLR4) were selected as hub genes. Immunological analysis suggested that 10 cell types (i.e., CD8/CD4 T cells) were lower in sepsis. Immune checkpoint-related genes CD274 (PD-L1), HAVCR2 (TIM3), and SIGLEC15 were overexpressed in sepsis. The AUROC for the diagnosis of sepsis for HMOX1 and TLR4 ranged from 0.77 to 0.81, while the AUROC of MAPK14 reached 0.935 and 0.941 in the training and validation sets. Serum ELISA results of HMOX1 and TLR4 showed no significant difference in differentiating sepsis. The AUROC of MAPK14 was 0.877. When the diagnostic threshold was 74.852 ng/ml, the sensitivity and specificity were 0.906 and 0.719, respectively.ConclusionFerroptosis-related gene MAPK14 is of considerable value in the early diagnosis of sepsis in children.

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Study of Monocyte Subsets and Their Surface Expression of CD86 and Serum IL-17 Compared to Serum Procalcitonin as Markers of Early Neonatal Sepsis

Cited by 7 publications

References 29 publications

Intermediate Monocytes with PD-L1 and CD62L Expression as a Possible Player in Active SARS-CoV-2 Infection

Intermediate Monocytes with PD-L1 and CD62L Expression as a Possible Player in Active SARS-CoV-2 Infection

Infiltrating circulating monocytes provide an important source of BMP4 at the early stage of spinal cord injury

Diagnostic and Predictive Values of Ferroptosis-Related Genes in Child Sepsis

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