Study of morbidity in orthotopic small intestine transplantation with Wistar rats: experimental study

Lee, André Dong Won; Gama-Rodrigues, Joaquim; Galvão, Flávio Henrique Ferreira; Waitzberg, Dan Linetzky

doi:10.1590/s0004-28032002000100008

Cited by 10 publications

(8 citation statements)

References 29 publications

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“…The success rate found in this study was 83.3%, which is comparable to those from literature. are also within the range reported in literature (15 to 25min 7,12 ; 45 to 67min 6,10,11 , 25min 9 and 113 to 120min 9,11 , respectively). Mean donor operation time in this study (40min) is shorter than found in others studies (57 12 , 81 11 and 89min 9 ).…”

Section: Clinical Indication Of Intestinal Transplantation Hassupporting

confidence: 90%

“…Although the varied outcome of rejection in outbred rats may be comparable to that observed in outbred large animals, including humans, these results demonstrate the unpredictability of long-term immunological response in transplantation models using outbred rats. In a previous report of our group 6 , we observed 41.1% (7 out of 17) of 30 days post-operative survival. These widely variable results corroborate the unpredictability of outbred rats long-term evolution.…”

Section: Clinical Indication Of Intestinal Transplantation Hassupporting

confidence: 44%

“…Small bowel transplantation in rats is a complex procedure that requires time-consuming education, mainly due to the use of microvascular anastomosis 4 , especially the venous one, which is a frequent site of thrombosis 6,7 . Various alternative SBT models have been therefore introduced.…”

Section: Clinical Indication Of Intestinal Transplantation Hasmentioning

confidence: 99%

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Small bowel transplantation in outbred rats

Waisberg¹,

Lee²,

Santos³

et al. 2011

Acta Cir. Bras.

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PURPOSE:To investigate the clinical evolution of orthotopic small bowel transplantation in outbred rats. METHODS:Seventy-two outbred Wistar rats weighting from 250 to 300g were used as donor and recipient in 36 consecutives ortothopic small intestine transplantation without immunosuppression. The graft was transplanted into the recipient using end-to-side aortic and portacaval microvascular anastomosis. Procedure duration, animal clinical course and survival were evaluated. Survival shorter than four days was considered technical failure. Recipients were sacrificed with signs of severe graft rejection or survival longer than 120 days. Necropsies were performed in all recipients to access histopathological changes in the graft. RESULTS: Median time for the procedure was 107 minutes. Six recipients (16.7%) presented technical failure. Twenty-seven recipients were sacrificed due to rejection, being nineteen (52.7%) between 7 th and 15 th postoperative day and eight (22.2%) between 34 th and 47 th postoperative day. Graft histology confirmed severe acute cellular rejection in those recipients. Uneventful evolution and survival longer than 120 days without rejection were observed in three recipients (8.3%). CONCLUSION:Intestinal transplantation in outbred rats without immunosuppressant regiment accomplishes variable clinical evolution. Keywords

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Section: Clinical Indication Of Intestinal Transplantation Hassupporting

confidence: 90%

Section: Clinical Indication Of Intestinal Transplantation Hassupporting

confidence: 44%

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Small bowel transplantation in outbred rats

Waisberg¹,

Lee²,

Santos³

et al. 2011

Acta Cir. Bras.

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“…In agreement with Lee et al, arterial anastomosis bleeding was the most common intra-operative complication in the recipient [5]. A successful vascular anastomosis is achieved following the microsurgery principles recommended by Guity et al [24].…”

Section: Discussionsupporting

confidence: 65%

“…In general terms, it is used to study intestinal ischemia-reperfusion injury, graft rejection and assess immunosuppressive therapies [2][3][4]. SBTx in rats is a feasible, replicable and inexpensive procedure when compared to large animals models [5].…”

Section: Introductionmentioning

confidence: 99%

Small bowel transplantation in rats, A multicenter experience summarizing the pitfalls to be overcome

Stringa¹,

Moreno²,

Lausada³

et al. 2017

Transplant Open

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Small Bowel transplantation in rats is a highly complex microsurgical procedure because several technical complications may lead to recipient mortality and transplant failure. Our aim was to report the most common complications associated with orthotopic and heterotopic intestinal transplantation in rats in order to identify the "pitfalls" of the procedure and prevent them. A retrospective multicenter study was performed. All participant centers have established rodent transplant procedures and trained surgeons. Two hundred ninety-three complications from 264 unsuccessful intestinal transplants were reported, representing an overall failure rate of 15% of the procedures performed. Recipient complications were most frequent than donor (257 vs. 36 p<0.0001). Excessive surgical time (11/36); severe hemorrhage (12/36) and inappropriate infusion of the preservation solution in the intestinal graft (11/36) were the most common donor complications. Arterial anastomosis bleeding (50/257), venous anastomosis bleeding (35/257) and portal vein stenosis (26/257) were the most common intraoperative complications in the recipient. To maximize success rate, surgeons should optimize time and avoid bleeding during graft dissection in the donor surgery. After performing a bloodless vascular anastomosis an adequate post-operative management of the animal is mandatory to guarantee survival.

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Two‐step procedure of whole orthotopic intestinal transplantation in rats: Considerations of techniques and graft functional adaptation

Li¹,

Zhu²,

Li³

2006

Microsurgery

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Intestinal transplantation may eventually become the definitive therapeutic modality for irreversible intestinal failure. To date, immunological, functional, and technical problems still hamper clinical success. In this paper, the technical aspects of a two-stage procedure for a whole orthotopic intestinal transplantation model of rats with systemic drainage are illustrated. Graft mucosal Na(+)/K(+)-ATPase and disaccharidase revealed recovery of graft function. From this study, we conclude that the present model may provide an excellent tool to further investigate the physiology and immunology of intestinal transplantation.

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Study of morbidity in orthotopic small intestine transplantation with Wistar rats: experimental study

Abstract: The experimented intestinal transplant is a procedure companied by considerable morbidity and mortality due to surgical complications in postoperative period, vascular anastomosis and total surgical time.

Cited by 10 publications

References 29 publications

Small bowel transplantation in outbred rats

Small bowel transplantation in outbred rats

Small bowel transplantation in rats, A multicenter experience summarizing the pitfalls to be overcome

Two‐step procedure of whole orthotopic intestinal transplantation in rats: Considerations of techniques and graft functional adaptation

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