Study of multiple solid-phase microextraction combined off-line with high performance liquid chromatography

Theodoridis, Georgios; Lontou, Maria Aikaterini; Michopoulos, Filippos; Suchá, Mária; Gondová, Taťána

doi:10.1016/j.aca.2004.04.012

Cited by 40 publications

(24 citation statements)

References 14 publications

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“…At the end of the extraction, the analyte was desorbed in 200 µL of methanol and an aliquot of 80 µL was injected to the HPLC. This method overcomes the problems that exist in conventional sample preparation techniques and can be applied to real urine samples [5].…”

Section: Solid Phase Microextraction For the Determination Of Antipsymentioning

confidence: 99%

“…For drug analysis of biological matrices the most common coatings are the polydimethylsiloxane (PDMS) and polyacrylate (PA) while other polymers such as polypyrrole coatings, coatings based on restricted access materials, and those based on mixtures of biocompatible polymers with sorbents used for SPE have also been developed and used [5,6].…”

Section: Solid Phase Microextraction For the Determination Of Antipsymentioning

confidence: 99%

“…Microextraction Determination Matrix Recovery Reference Haloperidol, quinine, naproxen, ciprofloxacin and paclitaxel SPME HPLC-UV Urine 85-95% [5] Risperidone and its biotransformation products SPME HPLC-MS/MS Liquid culture medium 11-28% [6] Clospirazine, fluphenazine, perazine, thiethylperazine, thioridazine, flupentixol, thioproperazine, trifluoperazine, perphenazine, prochlorperazine, propericiazine SPME Haloperidol Ultrasound-assisted emulsification microextraction.…”

Section: Analytementioning

confidence: 99%

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Recent Advances in Microextraction Techniques of Antipsychotics in Biological Fluids Prior to Liquid Chromatography Analysis

2017

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Antipsychotic drugs are a class of psychiatric medication worldwide used to treat psychotic symptoms principally in bipolar disorder, schizophrenia and other psycho-organic disorders. The traditional sample preparation techniques such as liquid-liquid extraction (LLE) or solid phase extraction (SPE), which were widely used, tend to have many drawbacks because they include complicated, time-consuming steps and they require large sample size as well large amounts of organic solvent. Therefore, due to the modern analytical requirements, such as miniaturization, automation and reduction of solvent volume and time, many microextraction procedures have been developed. In this review we aim to present an overview of those techniques which are used prior to liquid chromatography analyses both for forensic toxicology in different biological matrices as well as for therapeutic drug monitoring.

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Section: Solid Phase Microextraction For the Determination Of Antipsymentioning

confidence: 99%

Section: Solid Phase Microextraction For the Determination Of Antipsymentioning

confidence: 99%

Section: Analytementioning

confidence: 99%

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Recent Advances in Microextraction Techniques of Antipsychotics in Biological Fluids Prior to Liquid Chromatography Analysis

2017

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“…A literature search has shown several analytical methods for analysis of antipsychotic drugs, including haloperidol, in biological fluids (2,3), spectrophotometric methods for the determination of haloperidol (4), an adsorptive stripping voltammetry assay for determination of haloperidol in bulk form (5), an HPLC method for determination of haloperidol and its metabolites in plasma (6). Although some RP HPLC methods have been developed for the degradation study of haloperidol (7,8), there are no references in the literature concerning chemometrics approach to the development and validation of the RR RP-HPLC method for simultaneous determination of haloperidol and related compounds specified as impurities.…”

mentioning

confidence: 99%

Use of chemometrics for development and validation of an RP-HPLC method for simultaneous determination of haloperidol and related compounds

Petkovska¹,

Dimitrovska²

2008

Acta Pharmaceutica

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Haloperidol is one of the most widely used antipsychotic drugs in the treatment of schizophrenia, mania and other psychiatric disorders (1). Chemically, haloperidol (Hal) is 4-[4-(4-chlorophenyl)-4-hydroxy-1-piperidinyl]-1-(4-fluorophenyl)-1-butanone. The drug has six known related compounds specified as impurities: 4-(4-chlorophenyl)-4-hydroxy A rapid resolution reversed-phase high performance liquid chromatographic (RR RP-HPLC) method has been developed and validated for simultaneous determination of haloperidol and six related compounds. Investigated matrix was a laboratory mixture of a therapeutic active substance haloperidol and its six related compounds in concentration ratio 300:1. Experimental design was used during method optimization (full factorial 2 3 design) and robustness testing (Central Composite Circumscribed design). Three factors: organic phase variation during gradient elution, flow rate and gradient rise time were independent variables. To estimate the system response during the optimization procedure and robustness testing, resolution (R s ) and a chromatographic response function (CRF) were used. Chromatography was performed with the mobile phase containing phosphate buffer pH 6.5 and acetonitrile as organic modifier. Separation was achieved using gradient elution (organic phase fraction changed linearly from 20 to 72 %) over 7 min. A Zorbax Eclipse XDB C18 Rapid Resolution HT 4.6 mm´50 mm, 1.8 mm particle size, column was used at 25°C at a flow rate of 1.5 mL min -1 . UV detection was performed at 230 nm. The total time for chromatographic separation was 5.5 min with a total analysis time of 7.0 min. The method was validated for its linearity, precision, modal recovery and robustness.

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“…PDMS has been shown to absorb small hydrophobic molecules including pharmaceuticals like ciprofloxacin and paclitaxel [435]. Nile red, a hydrophobic fluorophore, was shown to strongly absorb into PDMS and could not be washed away from PDMS microchannels using detergent and/or water [433].…”

Section: Introductionmentioning

confidence: 99%

Device and application development for haematopoietic stem and progenitor cell (HSPC) and mesenchymal stromal cell (MSC) 3D spheroid cultures

Futrega¹

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KeywordsAIM 2: Utilization of the high throughput microwell platform to manufacture HSPC/MSC spheroids to improve the efficacy of direct BM transplantation.AIM 3: Development of an improved microwell platform that retains spheroids within discrete microwells throughout culture. AIM 4:Characterization of HSPC surface marker change in response to microwell material. DEVICE AND APPLICATION DEVELOPMENT FOR HSPC AND MSC 3D SPHEROID CULTURES iiiChapter 2 describes the development of a high throughput HSPC/MSC 3D spheroid co--culture embodied in AIM 1. I developed a high throughput polydimethylsiloxane (PDMS) microwell platform that enabled the manufacture of thousands of uniform 3D multicellular co--culture spheroids. Spheroids were assembled such that each contained 25--to--400 BM--derived MSC and 10 CB--derived CD34 + cells (a cell population enriched for HSPC). The outcomes from this Chapter guided the design of the subsequent three chapters. The specific outcomes and subsequent study results were as follows:Chapter 3: As described in Chapter 2, only modest improvements in expansion could be achieved by co--culturing HSPC in 3D MSC spheroids using a microwell platform. We reasoned that the 3D spheroids could, however, function as a biologically active anchor to improve direct BM transplantation outcomes. In this Chapter, we contrasted transplantation methods, including the use of MSC suspensions and 3D multicellular spheroids to modulate human CB--derived CD34 + cell retention within the BM cavity of NOD/SCID gamma (NSG) mice. Analysis revealed that the BM of injected femurs in some animals was saturated with human cells, while distal haematopoietic tissue engraftment was poor. These data show, for the first time, that retention of human haematopoietic cells within the BM enhances local engraftment at the expense of systemic engraftment.Chapter 4: As identified in Chapter 2, improvements in 3D spheroid co--cultures will require that the microwell platform is compatible with repeated medium exchange or dilution feeding. Multiple partial or complete medium exchanges can displace spheroids from discrete microwells, and this can result in either the loss of spheroids from culture, or spheroid amalgamation when displaced microtissues fall into common microwells. In this Chapter, I describe the first microwell platform that incorporates a mesh to retain microtissues within discrete microwells. We called this platform the microwell--mesh. We show that bonding a nylon mesh with an appropriate pore size over the microwell openings allows single cells to pass through the mesh into the microwells during the seeding process, but subsequently retains assembled microtissues within discrete microwells.Chapter 5: As identified in Chapter 2, the presence of PDMS, the material used to fabricate the microwell platform, altered CD38 surface expression on CB--derived CD34 + cells. Previous reports have shown that inhibition of retinoic acid signaling down--regulated CD38 iv DEVICE AND APPLICATION DEVELOPMENT FOR HSPC AND MSC 3D SPHE...

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Study of multiple solid-phase microextraction combined off-line with high performance liquid chromatography

Cited by 40 publications

References 14 publications

Recent Advances in Microextraction Techniques of Antipsychotics in Biological Fluids Prior to Liquid Chromatography Analysis

Recent Advances in Microextraction Techniques of Antipsychotics in Biological Fluids Prior to Liquid Chromatography Analysis

Use of chemometrics for development and validation of an RP-HPLC method for simultaneous determination of haloperidol and related compounds

Device and application development for haematopoietic stem and progenitor cell (HSPC) and mesenchymal stromal cell (MSC) 3D spheroid cultures

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