Study of natural ascorbigen and related compounds by HPLC

Александрова, Л. Г.; Королев, А. М.; Preobrazhenskaya, M. N.

doi:10.1016/0308-8146(92)90059-b

Cited by 42 publications

(25 citation statements)

References 14 publications

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“…3). So-called 'ascorbigen B' was later shown to be a mixture of indol-3-ylmethyl derivatives of ascorbigen (Aleksandrova et al 1992). The unexpected C-C connection between the ascorbic acid residue and the indol-3-ylmethyl residue in ascorbigen was explained by reaction with the keto form of ascorbate, with negatively charged C2 (Kiss and Neukom 1966).…”

Section: Formation and Reactions Of Ascorbigenmentioning

confidence: 98%

Indole glucosinolate breakdown and its biological effects

et al. 2008

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Section: Formation and Reactions Of Ascorbigenmentioning

confidence: 98%

Indole glucosinolate breakdown and its biological effects

et al. 2008

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“…It has been reported that ABG is not present in intact plant tissues (Preobrazhenskaya et al 1993) or it is present in low quantities in just shredded cabbage because a certain amount of GB might have been hydrolysed by the endogenous myrosinase as a consequence of cell damage (Ciska and Pathak 2004;Martinez-Villaluenga et al 2009;Peñas et al 2010). ABG formation is strongly dependent on the pH and the already formed ABG remains relatively stable at pH 4-5 characteristics of fermented cabbage products (Aleksandrova et al 1992). ABG is the most abundant indole GLS breakdown product in sauerkraut (Ciska and Pathak 2004;Agerbirk et al 1998), a plant food product that has been widely consumed for centuries.…”

Section: Introductionmentioning

confidence: 99%

Electrochemical Determination of Ascorbigen in Sauerkrauts

et al. 2011

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Ascorbigen (ABG), 2-C-(indol-3-yl)methyl-α-Lxylo-hex-3-ulofuranosono-1,4-lactone, was synthesised and its structure and purity was confirmed by means of 1 H NMR and 13 C NMR spectra and HPLC-PDA, respectively. The electrochemical behaviour of ABG was studied by cyclic voltammetry (CV) method for pH within the range of 3.0-7.0 and further characterisation was performed by differential pulse voltammetry (DPV) at pH 5.0. Voltammetric studies of ABG at glassy carbon electrode showed one irreversible oxidation peak (centred at E p =0.950 V for pH 5.0). The anodic peak current potential related to the irreversible oxidation of ABG was shifted towards more positive potentials with decreasing pH values. The linear response between concentration of ABG within the range of 0.08-0.75 mM and recorded current was observed. The DPV method was shown to be more sensitive when compared to the CV method. ABG showed a significant reducing activity provided by CV, whilst the antioxidant activity of ABG against O 2 −• was negligible. Electrochemical behaviour of ABG standard was applied successfully for the quantification of natural ABG in sauerkrauts by DPV and HPLC-CoulArray methods. Based on the developed analytical methods, ABG content increased during natural fermentation of cabbage at 0.5% and 1.5% NaCl levels, and results were comparable with those found in the literature.

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“…For qualitative and quantitative analysis of ascorbigens and other glucobrassicin hydrolysis products, different chromatographic (Aleksandrova et al, 1992;Buskov et al, 2000c;Kátay et al, 1996Kátay et al, , 2004Virtanen and Kiesvaara 1963) methods have been described. Kátay et al (1997) demonstrated the superiority of overpressured layer chromatography (OPLC) to conventional thin-layer chromatography (TLC) for separation of AG and MeAG, and its suitability for analysis of plant extracts (Kátay et al, 1998(Kátay et al, , 2002.…”

Section: Introductionmentioning

confidence: 99%

Potential role of formaldehyde in the mechanism of action of ascorbigens on the basis of BioArena studies

Kátay

Ott

Kátay

et al. 2008

Biomedical Chromatography

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The effect of ascorbigen and 1'-methylascorbigen as a model compound pair was studied on the phytopathogenic bacterium Pseudomonas savastanoi pv. phaseolicola in the BioArena experimental system after overpressured layer chromatography. Results showed a characteristic, strong antibacterial effect of 1'-methylascorbigen and weak effect of ascorbigen present on the adsorbent layer as chromatographic spot. Addition of formaldehyde capture compounds (L-arginine, glutathione, dimedone) partially or totally reduced the antibacterial effect of 1'-methylascorbigen and ascorbigen. On adding Cu(II) ions--which mobilize and coordinate formaldehyde--to the culture medium, the antibacterial effect of both compounds became stronger. It is supposed that the weak antibacterial effect of ascorbigen may have originated from the 1'-methylascorbigen formed in situ on the adsorbent layer by partial enzymatic methylation of ascorbigen.

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Study of natural ascorbigen and related compounds by HPLC

Cited by 42 publications

References 14 publications

Indole glucosinolate breakdown and its biological effects

Indole glucosinolate breakdown and its biological effects

Electrochemical Determination of Ascorbigen in Sauerkrauts

Potential role of formaldehyde in the mechanism of action of ascorbigens on the basis of BioArena studies

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