Study of natural ice-nucleating agents for cryopreservation of 3D tissue-engineered scaffolds

Mutsenko, Vitalii; Tarusin, Dmitriy N.; Sydykov, Bulat; Zaragotas, Dimitris; Simaioforidou, Anastasia; Rozanski, Corinna; Gryshkov, Oleksandr; Wolkers, Willem F.; Braslavsky, Ido; Anastassopoulos, Elias; Glasmacher, Birgit

doi:10.1016/j.cryobiol.2018.10.214

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“…In the current study, cryomicroscopic analysis has not been performed due to the large dimensions of the prepared core-shell capsules. In this context, the infrared thermography that provides non-invasive monitoring of multiple freezing/thawing events may be very informative and instrumental for the high-throughput analysis of core-shell capsules during cryopreservation [ 74 , 75 ].…”

Section: Discussionmentioning

confidence: 99%

Coaxial Alginate Hydrogels: From Self-Assembled 3D Cellular Constructs to Long-Term Storage

Gryshkov

Mutsenko

Tarusin

et al. 2021

IJMS

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Alginate as a versatile naturally occurring biomaterial has found widespread use in the biomedical field due to its unique features such as biocompatibility and biodegradability. The ability of its semipermeable hydrogels to provide a favourable microenvironment for clinically relevant cells made alginate encapsulation a leading technology for immunoisolation, 3D culture, cryopreservation as well as cell and drug delivery. The aim of this work is the evaluation of structural properties and swelling behaviour of the core-shell capsules for the encapsulation of multipotent stromal cells (MSCs), their 3D culture and cryopreservation using slow freezing. The cells were encapsulated in core-shell capsules using coaxial electrospraying, cultured for 35 days and cryopreserved. Cell viability, metabolic activity and cell–cell interactions were analysed. Cryopreservation of MSCs-laden core-shell capsules was performed according to parameters pre-selected on cell-free capsules. The results suggest that core-shell capsules produced from the low viscosity high-G alginate are superior to high-M ones in terms of stability during in vitro culture, as well as to solid beads in terms of promoting formation of viable self-assembled cellular structures and maintenance of MSCs functionality on a long-term basis. The application of 0.3 M sucrose demonstrated a beneficial effect on the integrity of capsules and viability of formed 3D cell assemblies, as compared to 10% dimethyl sulfoxide (DMSO) alone. The proposed workflow from the preparation of core-shell capsules with self-assembled cellular structures to the cryopreservation appears to be a promising strategy for their off-the-shelf availability.

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Section: Discussionmentioning

confidence: 99%