“…While NS0 cells normally require serum as a cholesterol source, several research groups have demonstrated the growth of NS0 cells in protein free and chemically defined medium, using chemically defined cholesterol supplements. (Zhang & Robinson, 2005;Talley et al 2005;Ojito et al 2001) The auxotrophic nature of NS0 cells with respect to cholesterol makes this cell line a natural choice for characterizing the bioavailability of cholesterol nanoparticles produced by electrospray.…”