Study of optimization of wastewater contaminant removal along with extracellular polymeric substances (EPS) production by a thermotolerant Bacillus sp. ISTVK1 isolated from heat shocked sewage sludge

Gupta, Asmita; Thakur, Indu Shekhar

doi:10.1016/j.biortech.2016.02.040

Cited by 53 publications

(15 citation statements)

References 24 publications

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“…Furthermore, the hydrophobic regions and numerous anionic functional groups (e.g., carboxyl, phosphoryl, sulfhydryl, phenyl, and hydroxyl) present in the structure of EPS not only mediate the accumulation of nutrients but also bind the contaminants present in wastewater including heavy metals [83,84]. This was confirmed by Gupta and Thakur [85] who reported that the effectiveness of contaminant removal from wastewater inoculated with Bacillus sp. ISTVK1 was partially associated with its ability to produce a copious number of extracellular polymers.…”

Section: Discussionmentioning

confidence: 71%

Selecting Bacteria Candidates for the Bioaugmentation of Activated Sludge to Improve the Aerobic Treatment of Landfill Leachate

Michalska

Piński

Żur

et al. 2020

Water

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In this study, a multifaceted approach for selecting the suitable candidates for bioaugmentation of activated sludge (AS) that supports leachate treatment was used. To determine the exploitation of 10 bacterial strains isolated from the various matrices for inoculating the AS contaminated with the Kalina pond leachate (KPL), their degradative potential was analyzed along with their aptitude to synthesize compounds improving remediation of pollutants in wastewater and ability to incorporate into the AS flocs. Based on their capability to degrade aromatic compounds (primarily catechol, phenol, and cresols) at a concentration of 1 mg/mL and survive in 12.5% of the KPL, Pseudomonas putida OR45a and P. putida KB3 can be considered to be the best candidates for bioaugmentation of the AS among all of the bacteria tested. Genomic analyses of these two strains revealed the presence of the genes encoding enzymes related to the metabolism of aromatic compounds. Additionally, both microorganisms exhibited a high hydrophobic propensity (above 50%) and an ability to produce biosurfactants as well as high resistance to ammonium (above 600 µg/mL) and heavy metals (especially chromium). These properties enable the exploitation of both bacterial strains in the bioremediation of the AS contaminated with the KPL.

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Section: Discussionmentioning

confidence: 71%

Selecting Bacteria Candidates for the Bioaugmentation of Activated Sludge to Improve the Aerobic Treatment of Landfill Leachate

Michalska

Piński

Żur

et al. 2020

Water

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“…ISTVK1 (0.31 g L −1 ) on waste water and sucrose, Proteus mirabilis TJ-1 (1.3 g L −1 ) on glucose, Streptococcus thermophilus ASCC 1275 (1 g L −1 ) in milk, Klebsiella sp. (1.0 g L −1 ) from glucose and P. jamilae CP-38 (4.2 g L −1 ) on olive mill wastewater 12,35,36 . Compared to earlier reports, this strain produced a good amount of EPS and that too using CO 2 and glucose as carbon source.…”

Section: Resultsmentioning

confidence: 99%

Genomic analysis of carbon dioxide sequestering bacterium for exopolysaccharides production

Kumar

Pandey

et al. 2019

Sci Rep

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In the present study, genomic analysis of a previously reported carbon dioxide (CO2) sequestering bacterium Serratia sp. ISTD04 was performed along with exopolysaccharide (EPS) production. Genomic analysis identified key and accessory enzymes responsible for CO2 sequestration. EPS synthesis genes were discovered in the genome and identified 8 putative clusters responsible for lipopolysaccharide, stewartan, emulsan, polysaccharide B, capsular polysaccharide and fatty acid-saccharide production. The production of EPS was found to be 0.88 ± 0.08, 1.25 ± 0.13 and 1.44 ± 0.10 g L−1 on glucose, bicarbonate (NaHCO3) and NaHCO3 plus glucose respectively at pH 7.8. After optimizing process parameters, the EPS production increased more than 3 folds. The morphology of strain and elemental composition of EPS was characterized by SEM-EDX. The functional groups, monomer composition, linkage analysis and structure of purified EPS was characterized by FTIR, GC-MS and 1H and 13C NMR. Glucose, galactose, mannose and glucosamine are the monomers detected in the EPS. EPS was further applied for bioflocculation (kaolin test) and dye removal. The EPS showed 68% ± 0.9 flocculating activity and decolorized cationic dye acridine orange (80%) and crystal violet (95%). The results highlight CO2 sequestration and EPS production potential of Serratia sp. ISTD04 that can be harnessed in future.

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“…Similar results were reported by More et al [1], where 2.3 g/L of EPS (after 72 h) with a maximum flocculation activity of 79.1% was obtained when autoclaved wastewater sludge was used as substrate (Table 1). Gupta et al [7] also reported EPS production ( Table 1) when 50% of sterilised wastewater was used as a substrate by Bacillus sp. ISTVK1.…”

Section: Introductionmentioning

confidence: 94%

“…According to Gupta and Thakur [7], the use of cheap carbon and nutrient sources for large scale EPS production can help raise the benefit-cost ratio thus making the product marketable. Therefore, the main challenges in these processes are the production of high yields of EPS by bacteria and the use of a low carbon source as a substrate.…”

Section: Flocculation Activity Of Epsmentioning

confidence: 99%

Wastewater Valorization by Pure Bacterial Cultures to Extracellular Polymeric Substances (EPS) with High Emulsifying Potential and Flocculation Activities

Drakou

Amorim

Castro

et al. 2017

Waste Biomass Valor

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Study of optimization of wastewater contaminant removal along with extracellular polymeric substances (EPS) production by a thermotolerant Bacillus sp. ISTVK1 isolated from heat shocked sewage sludge

Cited by 53 publications

References 24 publications

Selecting Bacteria Candidates for the Bioaugmentation of Activated Sludge to Improve the Aerobic Treatment of Landfill Leachate

Selecting Bacteria Candidates for the Bioaugmentation of Activated Sludge to Improve the Aerobic Treatment of Landfill Leachate

Genomic analysis of carbon dioxide sequestering bacterium for exopolysaccharides production

Wastewater Valorization by Pure Bacterial Cultures to Extracellular Polymeric Substances (EPS) with High Emulsifying Potential and Flocculation Activities

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