Study of phylogenetic relationship of Turkish species ofMatthiola (Brassicaceae) based on ISSR amplification

Abstract

IntroductionBrassicaceae, the vascular plant family, consists of 321 genera and 3660 species (Al-Shehbaz, 2012). The cosmopolitan Brassicaceae family is abundant in the northern hemisphere. Currently, there are 49 recognized Brassicaceae tribes into which more than 90% of the genera have been allocated based on molecular analysis (Warwick et al., 2010).A recently published study titled "The Families and Genera of the Vascular Plants" reported that the genus Matthiola is represented by 50 species worldwide, but most of them are found in Eurasia and Africa (Appel and Al-Shehbaz, 2003).Matthiola has 10 taxa in the flora of Europe (Ball, 1964), 6 in the flora of Palestine (Zohary, 1966), 17 in the flora of Iran (Rechinger, 1968), and 15 in the flora of the former USSR (Komarov, 1977). Matthiola has 10 species in Turkey (Mutlu, 2012). The taxa Matthiola ovatifolia (Boiss.) Boiss. and Matthiola trojana have recently been added to the flora of Turkey (Davis, 1988;Dirmenci et al., 2006).Molecular methods have an important role in the identification of phylogenetic relationships within Brassicaceae. Molecular phylogenetic studies have contributed significantly to the generic delimitation on this family (

A Study on Determination of The Fatty Acid Composition of Cucurbita pepo L. Seeds

“…This result was the same in a study [18,19] and lower than the other one [20]. The reason of differences that in the studies could be by the taxonomic or eco-geographical differences [21]. Value of linoleic acid and arachidic acid were lower that some of studies about Cucurbita pepo L. [18].…”

First Report on Development of Genome-Wide Microsatellite Markers for Stock (Matthiola incana L.)

“…Genetic analysis techniques based on DNA molecular markers can provide an efficient and accurate representation of a species’ genetic potential, regardless of age, physiological conditions, and environmental factors, thus helping to effectively characterize and utilize germplasm resources in breeding [ 8 , 9 ]. DNA molecular markers such as RFLP [ 10 ], RAPD [ 8 , 11 , 12 ], AFLP [ 13 ], ISSR [ 11 , 14 ] and SSR [ 8 , 12 ] are being widely used for genetic research, such as genetic mapping, marker-assisted selection breeding, and genetic diversity, etc., [ 15 ]. There are few reports on study using DNA molecular markers; among the limited reports, Dogan et al (2016) used ISSR markers to study the phylogenetic relationship between Matthiola and other related genera [ 14 ].…”

“…DNA molecular markers such as RFLP [ 10 ], RAPD [ 8 , 11 , 12 ], AFLP [ 13 ], ISSR [ 11 , 14 ] and SSR [ 8 , 12 ] are being widely used for genetic research, such as genetic mapping, marker-assisted selection breeding, and genetic diversity, etc., [ 15 ]. There are few reports on study using DNA molecular markers; among the limited reports, Dogan et al (2016) used ISSR markers to study the phylogenetic relationship between Matthiola and other related genera [ 14 ]. Nakatsuka and Koishi (2018) developed specific codominant DNA molecular markers [ 16 ], and Ho et al (2021) used RAPD and ISSR markers to identify double flowered plants [ 11 ].…”

“…As the most useful DNA marker system for variety identification and germplasm management, SSR molecular markers have several advantages due to their abundance, high polymorphism, multiple alleles, co-dominance, low-cost, and the ease of assay by PCR [ 17 , 18 ], which have been applied extensively in genetic analysis, such as the analysis of population structure and genetic diversity [ 19 , 20 ], QTL mapping [ 21 , 22 , 23 ], marker-assisted selection breeding [ 24 , 25 , 26 ], and DNA fingerprinting [ 27 , 28 ], etc. SSR molecular markers are rarely used in M. incana [ 11 , 14 ], which is largely due to the fact that SSR molecular markers have not been developed in M. incana . Early development methods for SSR tagging suffered from cumbersome operations, low success rates and high-costs, or were limited by the information in public data.…”

Genetic Diversity of Elaeagnus angustifolia L. (Elaeagnaceae) Populations in İzmir (Turkey)

“…ISSR (Inter-simple sequence repeats) technique, is applicable to organisms from diverse genera and because of the large number of primers available for analyses, it provides good overall genome coverage (Andiego et al, 2019). This technique, has been successfully used in plant genetics for genome fingerprinting, genetic diversity, phylogenetic analysis, and molecular breeding in marker assisted selection (Doğan et al, 2016;Tang et al, 2015;Son et al, 2012). The aim of this study was to perform a genetic diversity using ISSR markers for some E. angustifolia populations grown in the İzmir city of Turkey.…”