Study of Phytochemicals of <i>Melilotus indicus</i> and Alpha‐Amylase and Lipase Inhibitory Activities of Its Methanolic Extract and Fractions in Different Solvents

Iftikhar, Haroon; Ahmed, Dildar; Qamar, Muhammad Tariq

doi:10.1002/slct.201901120

Cited by 12 publications

(7 citation statements)

References 15 publications

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“…Among all the extracts, the MeOH extract exhibited the most potent α-amylase inhibitory action. This extract inhibited the α-amylase activity in a dose-dependent manner ( Figure 3 , Table 3 ), consistent with previous reports that α-amylase inhibitory potential was higher in more polar plant extracts [ 32 , 57 , 58 ]. Thus, the enzyme inhibitory potential of the MeOH extract could be due to the presence of polyphenols, flavonoids, and glycosides.…”

Section: Discussionsupporting

confidence: 91%

Secondary Metabolite Profiling, Antioxidant, Antidiabetic and Neuroprotective Activity of Cestrum nocturnum (Night Scented-Jasmine): Use of In Vitro and In Silico Approach in Determining the Potential Bioactive Compound

Ahmad

Alrouji

Alhajlah

et al. 2023

Plants

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This study aims to describe the therapeutic potential of C. nocturnum leaf extracts against diabetes and neurological disorders via the targeting of α-amylase and acetylcholinesterase (AChE) activities, followed by computational molecular docking studies to establish a strong rationale behind the α-amylase and AChE inhibitory potential of C. nocturnum leaves-derived secondary metabolites. In our study, the antioxidant activity of the sequentially extracted C. nocturnum leaves extract was also investigated, in which the methanolic fraction exhibited the strongest antioxidant potential against DPPH (IC50 39.12 ± 0.53 µg/mL) and ABTS (IC50 20.94 ± 0.82 µg/mL) radicals. This extract strongly inhibited the α-amylase (IC50188.77 ± 1.67 µg/mL) and AChE (IC50 239.44 ± 0.93 µg/mL) in a non-competitive and competitive manner, respectively. Furthermore, in silico analysis of compounds identified in the methanolic extract of the leaves of C. nocturnum using GC-MS revealed high-affinity binding of these compounds with the catalytic sites of α-amylase and AChE, with binding energy ranging from −3.10 to −6.23 kcal/mol and from −3.32 to −8.76 kcal/mol, respectively. Conclusively, the antioxidant, antidiabetic, and anti-Alzheimer activity of this extract might be driven by the synergistic effect of these bioactive phytoconstituents.

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Section: Discussionsupporting

confidence: 91%

Secondary Metabolite Profiling, Antioxidant, Antidiabetic and Neuroprotective Activity of Cestrum nocturnum (Night Scented-Jasmine): Use of In Vitro and In Silico Approach in Determining the Potential Bioactive Compound

Ahmad

Alrouji

Alhajlah

et al. 2023

Plants

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“…So, oleanolic acid is responsible for inhibition of ACE by MBLE. In addition, moderate inhibition of α-amylase and pancreatic lipase by MBLE was also found here and according to the previous reports, this observed inhibitory property may be due to the presence of hexadecanoic acid methyl ester, 8,11octa-decadienoic acid methyl ester and 3,7,11,15-tetramethyl-2-hexadecen-1-ol (Figure 2) in MBLE [38][39][40] . Thus, some of the identified compounds may play their key role in the inhibition of enzymes by MBLE.…”

Section: Chemical Composition Analysis By Gc-mssupporting

confidence: 90%

Phytochemical composition of Blumea lacera leaf and its inhibitory effects on the activity of enzymes related to metabolic diseases

Habib¹,

Igarashi²,

Rabbi³

2022

Pharm Sci Asia

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Blumea lacera (Bum. f.) DC is an important medicinal plant of Bangladesh having several ethnomedicinal values. To give the scientific basis of ethnomedicinal uses, the present study analyzed phytochemical composition of methanol extract of B. lacera leaves (designated as MBLE) and evaluated its inhibitory effects on pancreatic lipase, α-amylase, xanthine oxidase (XO) and angiotensin I-converting enzyme (ACE). Different in vitro methods were used to perform enzyme inhibition assays for MBLE and its phytochemical profile was analyzed by GC-MS. In case of all the enzyme inhibition assays, the percentage of inhibition by MBLE was increased with increasing in concentrations. Among these enzymes, MBLE showed strong activity (IC50:13.34±1.05 μg/mL) against ACE as compared with captopril (IC50:8.09±0.74 μg/mL) used as standard reference whereas it exhibited moderate activity against other enzymes. The IC50 values of MBLE were found to be 9.87±0.25, 40.17±2.32 and 93.88±5.21 μg/mL for pancreatic lipase, α-amylase and XO, respectively. In addition, some compounds identified in MBLE by GC-MS, were also consistence with these enzyme inhibitory activities. Thus, this study demonstrates the enzyme inhibitory potentials of B. lacera leaves for the first time and it might be a potential tool for the treatment of metabolic diseases.

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“…EI mass spectra were collected at 70 eV ionization voltage over the range of m/z 50–650 in full scan mode, and the ion source temperature was set to 250 °C. The components were identified by comparing their retention times and mass spectra using the Wiley Spectral library search program [ 12 ].…”

Section: Methodsmentioning

confidence: 99%

A Preliminary Study on the Effect of Psyllium Husk Ethanolic Extract on Hyperlipidemia, Hyperglycemia, and Oxidative Stress Induced by Triton X-100 Injection in Rats

Hashem

Abd-Allah

Mahmoud

et al. 2021

Biology

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The aim of this study is to assess the efficiency of psyllium husk ethanolic extract (PHEE) on Triton X-100 induced hyperlipidemic rats by studying the changes in hepatic and pancreatic function and histopathology. Forty male albino rats (bodyweight 175–188 g) were grouped randomly into four sets with ten rats. The experimental groups included: (1) control group (CON); (2) Triton X-100 induced hyperlipidemic group—rats were intraperitoneally injected with a single dose of Triton X-100 (100 mg/kg body weight) on the 21st day of Trial onset; (3) PHEE group—PHEE was orally administered (100 mg/kg body weight dissolved in 1 mL of distilled water) by gastric tube from the first day of the experiment until the fortieth day, once daily, (PHEE); (4) PHEE +Triton group, which received PHEE orally with the induction of hyperlipidemia. Treating hyperlipidemic rats with PHEE showed a decrease in the total serum lipids, triglyceride (TG), total cholesterol (TC), atherogenic index (AI), and malondialdehyde (MDA) with an increase in superoxide dismutase (SOD) and catalase (CAT) activities. PHEE administration alleviated the negative impact of Triton on the serum levels of glucose, insulin, glycated hemoglobin (HbA1c), homeostatic model assessment for insulin resistance (HOMA IR index), leptin hormone, Alanine Aminotransferase (ALT), Aspartate Aminotransferase (AST), Gamma-Glutamyl Transferase (GGT) and proteinogram. The Triton-induced hyperlipidemic rats showed extensive histopathological changes in the liver and pancreas, which were alleviated with PHEE administration. It could be concluded that PHEE has potent effects against hyperlipidemia, hyperglycemia, and oxidative stress due to its biologically active constituents detected by GC-MS analysis. This study’s findings may help develop a novel trial against the effects of hyperlipidemia in the future.

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Study of Phytochemicals of Melilotus indicus and Alpha‐Amylase and Lipase Inhibitory Activities of Its Methanolic Extract and Fractions in Different Solvents

Cited by 12 publications

References 15 publications

Secondary Metabolite Profiling, Antioxidant, Antidiabetic and Neuroprotective Activity of Cestrum nocturnum (Night Scented-Jasmine): Use of In Vitro and In Silico Approach in Determining the Potential Bioactive Compound

Secondary Metabolite Profiling, Antioxidant, Antidiabetic and Neuroprotective Activity of Cestrum nocturnum (Night Scented-Jasmine): Use of In Vitro and In Silico Approach in Determining the Potential Bioactive Compound

Phytochemical composition of Blumea lacera leaf and its inhibitory effects on the activity of enzymes related to metabolic diseases

A Preliminary Study on the Effect of Psyllium Husk Ethanolic Extract on Hyperlipidemia, Hyperglycemia, and Oxidative Stress Induced by Triton X-100 Injection in Rats

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