Study of preparations of bee pollen extracts, antioxidant and antibacterial activity

Abstract

The aim of this study was to determine the antioxidant activity, phenolic content and antibacterial activity of pollen extracts obtained with different concentrations of ethanol. Each extraction condition (ethanol solutions from 40 to 90%) had a different effect in the phenolic compounds content. Although, the pollen extract obtained at 60, 70 and 80% of ethanol showed relatively higher levels of phenolic compounds (>10 mg/g) and did not present statistical significant difference between the extraction conditions. The amount of total phenolics ranged from 3.6 to 8.1 and 6.6 to 10.9 mg GAE/g for Alagoas state and Parana state pollen, respectively. The higher value for antioxidant activity index was 83.30% for the pollen from Alagoas state and 81.15 % for Parana state pollen. The highest degree of antioxidant activity was found in the extraction at 60% of ethanol solution for Parana state pollen, which also showed the highest concentration of polyphenol compounds. Staphylococcus aureus was inhibited by the ethanolic extract of Alagoas state pollen in all the concentrations of solvent, except the ethanolic extract of pollen at 90%. The extract at 60% of ethanol solution (Parana sample) inhibited Bacillus subtilis, Pseudomonas aeruginosa and Klebsiella sp.Index terms: Pollen, antioxidant, antibacterial activity. RESUMOObjetivou-se, neste estudo determinar compostos fenólicos, a atividade antioxidante a antibacteriana dos extratos etanólicos de pólen, obtidos com diferentes concentrações de etanol. As diferentes condições de extração (etanol de 40 a 90%) apresentaram diferentes efeitos no conteúdo dos compostos fenólicos extraídos. Os extratos de pólen obtidos com etanol a 60, 70 e 80% apresentaram maiores níveis de compostos fenólicos (>10 mg/g) e não apresentaram diferenças estatísticas entre essas condições de extração. A quantidade de compostos fenólicos nos extratos de pólen variou de 3.6 a 8.1 e 6.6 a 11 mg de equivalente em ácido gálico por g de pólen (GAE), para os estados de Alagoas e Paraná, respectivamente. Os maiores índices de atividade antioxidante para o pólen do estado de Alagoas foi de 83.30% e 81.15 % para o pólen do estado do Paraná. O pólen do estado do Paraná extraído com etanol a 60%, apresentou o maior índice de atividade antioxidante e também a maior concentração de compostos fenólicos. A bactéria Staphylococcus aureus foi inibida pelo extrato etanólico do pólen de Alagoas, em todas as concentrações, exceto no extrato etanólico a 90%. O extrato etanólico a 60%, no pólen do Paraná, inibiu Bacillus subtilis, Pseudomonas aeruginosa e Klebsiella sp.Termos para indexação: Pólen apícola, atividade antioxidante e antibacteriana.

Physicochemical composition and antioxidant potential of bee pollen from different botanical sources in Alagoas, Brazil

“…The extracts were obtained according to the methodology described by Carpes et al (2007) with modifications. Briefly, 1 g of pollen from each of the ten apiary samples was mixed together.…”

“…The content of total phenolic compounds was determined using the spectrophotometric method of FolinCiocalteu (FC) modified by Carpes et al (2007) using a standard curve (linear regression of the absorbance) of gallic acid (0.5-100 µg/mL). Aliquots (2.5 mL) of FC reagent were added to 0.5 mL of diluted HPE (1:100), and 5 min later, 2.0 mL of 4% (w/v) sodium carbonate were added (Na 2 CO 3 ).…”

A Comparative Study on Propolis and Pollen Extracts: Chemical Profile Analysis, Antioxidant and Anticancer Activity

“…However, our results have similarity with the data obtained by Basuny et al (2013) who found that IC 50 for palm pollen extract was 0.62 mg/mL also with Carpes et al (2009) who found that IC 50 for pollen ranged from 0.8 to 4.69 mg/mL for pollen collected from Southern Brazil. Also, highest degree of antioxidant activity was found in the extraction at 60% of ethanol solution for Parana state pollen, which also showed the highest concentration of polyphenol compounds (Carpes et al, 2007). About the mixtures of propolis and pollen extracts, the IC 50 is generally moderate between pollen and propolis and this suggested that these mixtures can be used also as a potent antioxidant, which gathered the functional and pharmacological activities of both extracts.…”

“…Extracts of bee products was prepared as the method described by Vongsak et al (2015) and Carpes et al (2007) with some modifications. each bee product sample (30g) were milled, homogenized and extracted individually using 300 mL of ethanol as extraction solvent in different concentrations ( 98,70,0%) at temperature of 50ºC for 30 min with constant agitation.…”

“…The bioactive properties of apicultural propolis and pollen extracts can be increased using a solvent suitable for its extraction, improving the activity of free radicals sequestration (antirust activity) (Carpes et al, 2007;Sun et al, 2015). So our aims of this study were to investigate the bioactive compounds in pollen and propolis extracts which obtained by different ethanol/water solvent and evaluating the antioxidant and anticancer activity of these extracts.…”