Study of radiation induced changes of phosphorus metabolism in mice by 31P NMR spectroscopy

Serša, Igor; Kranjc, Simona; Serša, Gregor; Nemec-Svete, Alenka; Lozar, Bojan; Sepe, Ana; Vidmar, Jernej; Šentjurc, Marjeta

doi:10.2478/v10019-010-0030-z

Cited by 13 publications

(4 citation statements)

References 26 publications

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“…When these atoms are subjected to a magnetic field, they absorb an electromagnetic wave and resonate by emitting an energy peak detectable with a NMR spectrometer [93]. Since ATP, ADP and AMP (Adenosine Monophosphate) molecules contain a significant proportion of H and P atoms, they behave as magnetic dipoles and it is possible to detect them using this approach [94][95][96] (Figure 7).…”

Section: Nuclear Magnetic Resonance Spectroscopymentioning

confidence: 99%

Approaches to monitor ATP levels in living cells: where do we stand?

Ley-Ngardigal

Bertolin

2021

The FEBS Journal

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ATP is the most universal and essential energy molecule in cells. This is due to its ability to store cellular energy in form of high energy phosphate bonds, which are extremely stable and readily usable by the cell. This energy is key for a variety of biological functions such as cell growth and division, metabolism, signaling, and for the turnover of biomolecules. Un-ACCEPTED MANUSCRIPT -CLEAN COPY 2 derstanding how ATP is produced and hydrolyzed with a spatiotemporal resolution is necessary to understand its functions both in physiological and pathological contexts. In this review, we will first describe the organization of the electron transport chain and ATP synthase, the main molecular motor for ATP production in mitochondria. Second, we will review the biochemical assays currently available to estimate ATP quantities in cells, and we will compare their readouts, strengths and weaknesses. Finally, we will explore the palette of geneticallyencoded biosensors designed for microscopy-based approaches, and show how their spatiotemporal resolution opened up the possibility to follow ATP levels in living cells.

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Section: Nuclear Magnetic Resonance Spectroscopymentioning

confidence: 99%

Approaches to monitor ATP levels in living cells: where do we stand?

Ley-Ngardigal

Bertolin

2021

The FEBS Journal

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“…When these atoms are subjected to a magnetic field, they absorb an electromagnetic wave and resonate by emitting an energy peak detectable with a NMR spectrometer [35]. Since ATP, ADP and AMP molecules contain a significant proportion of H and P atoms, it is possible to detect them using this approach [36][37][38] (Figure 6).…”

Section: Nuclear Magnetic Resonance Spectroscopymentioning

confidence: 99%

Approaches to Monitor ATP Levels in Living Cells: Where Do We Stand?

Ley-Ngardigal¹,

Bertolin²

2021

Preprint

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ATP is the most universal and essential energy molecule in the eukaryotic cell. This is due to its ability to store energy in form of high energy phosphate bonds, which are extremely stable and readily usable by the cell. This energy is key for a variety of biological functions such as cell growth and division, metabolism, signalling, and for the turnover of biomolecules. Understanding how ATP is produced and hydrolysed with a spatiotemporal resolution is necessary to understand its functions both in physiological and pathological contexts. In this review, we will first describe the ATP synthase, the main molecular motor for ATP production in mitochondria. Second, we will review the biochemical assays currently available to estimate ATP quantities in cells, and we will compare their readouts, strengths and weaknesses. Then, we will explore the palette of genetically-encoded biosensors designed for microscope-based approaches and show how their spatiotemporal resolution opened up the possibility to follow ATP levels and production in living cells. Finally, we will comment on how ATP monitoring is used in preclinical practices, and to what extent genetically-encoded sensors could be used as a promising tool to elucidate pathologies in which ATP is implicated.

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“…Irradiation alters the level of various parenchymal intracellular enzyme such as aspartate aminotransferase and alanine aminotransferase (Almonte et al, ). Functional impairment of these enzymes ultimately leads to hepatocellular injury (Sersa et al, ). Contrary to easily manageable intracellular oxidative stress, exposure to gamma irradiation induces several magnitudes of highly unmanageable oxidative stress in the cells.…”

Section: Introductionmentioning

confidence: 99%

Semiquinone fraction isolated fromBacillussp. INM-1 protects hepatic tissues against γ-radiation induced toxicity

et al. 2013

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Present study was focused on evaluation of a semiquinone glucoside derivative (SQGD) isolated from radioresistant bacterium Bacillus sp. INM-1 for its ability against γ radiation induced oxidative stress in irradiated mice. Animals were divided into four group, i.e., (i) untreated control mice; (ii) SQGD treated (50 mg/kg b. wt. i.p.) mice; (iii) irradiated (10 Gy) mice; and (iv) irradiated mice which were pre-treated (-2 h) with SQGD (50 mg/kg b. wt. i.p.). Following treatment, liver homogenates of the treated mice were subjected to endogenous antioxidant enzymes estimation. Result indicated that SQGD pre-treatment, significantly (P < 0.05) induced superoxide dismutase (SOD) (19.84 ± 2.18% at 72 h), catalase (CAT) (26.47 ± 3.11% at 12 h), glutathione (33.81 ± 1.99% at 24 h), and glutathione-S-transferase (24.40 ± 2.65% at 6 h) activities in the liver of mice as compared with untreated control. Significant (P < 0.05) induction in SOD (50.04 ± 5.59% at 12 h), CAT (62.22 ± 7.50 at 72 h), glutathione (42.92 ± 2.28% at 24 h), and glutathione-S-transferase (46.65 ± 3.25 at 12 h) was observed in irradiated mice which were pre-treated with SQGD compared with only irradiated mice. Further, significant induction in ABTS(+) radicals (directly proportional to decrease mM Trolox equivalent) was observed in liver homogenate of H2 O2 treated mice which were found to be significantly inhibited in H2 O2 treated mice pre-treated with SQGD. Thus, it can be concluded that SQGD treatment neutralizes oxidative stress caused by irradiation not only by enhancing endogenous antioxidant enzymes but also by improving total antioxidant status of cellular system and thus cumulative effect of the phenomenon may contributes to radioprotection.

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Study of radiation induced changes of phosphorus metabolism in mice by 31P NMR spectroscopy

Cited by 13 publications

References 26 publications

Approaches to monitor ATP levels in living cells: where do we stand?

Approaches to monitor ATP levels in living cells: where do we stand?

Approaches to Monitor ATP Levels in Living Cells: Where Do We Stand?

Semiquinone fraction isolated fromBacillussp. INM-1 protects hepatic tissues against γ-radiation induced toxicity

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