Study of Recombinant Protein Fractions From the Bombyx Mori and Pichia Pastoris Using "Native Page" Method

Abstract

We have studied the recombinant protein fractions of ̴ 24-34 kDa obtained from Bombyx mori - silkworm larvae and yeast Pichia pastoris. Fractions, containing the recombinant proteins by gel-electrophoresis using the “Native PAGE” method, which allows for differentiation of proteins according to their natural (surface) charge and conformational properties were studied. The results showed that the recombinant protein fraction from Bombyx mori (̴ 34 kDa) consisted of four components and fraction from Pichia pastoris (̴ 24 kDa) consisted one component. The most optimal conditions for the “Native PAGE”- the main gel: Tris HCl (pH-8.8) 244 mM, Bis-Acrylamide 10%, TEMED 0.08%, APS 0.08%; concentration gel: Tris HCl (pH-6.8) 125 mM, Bis-Acrylamide 4%, TEMED 0.05%, APS 0.05% were found.