Study of Resistance of Aedes aegypti Mosquitoes to Pyrethroids by Free-flight Method

Abstract

Efforts to combat dengue hemorrhagic fever (DHF) in Bali are focused on controlling the vector of Aedes aegypti Mosquitoes. One method is fumigation (fogging) with insecticides. The use of insecticides for a long time and improper doses can trigger resistance. Many countries including Indonesia have reported incidents of insecticide resistance. Methods for determining the level of insecticide resistance are bioassay, biochemistry, and gene mutation. The method does not describe the actual environmental conditions of Mosquitoes. Queensland Institute of Medical Research (QIMR) Berghofer developed a free-flight test procedure to evaluate insecticide resistance in free-flying mosquito conditions. The study, which was conducted in June 2019-January 2020, aimed to measure the level of insecticide resistance with the free-flight method in the A. aegypti population in North Denpasar. A total of 20 female A. aegypti aged 5-10 days were released in the free-flight room for 24 hours with permethrin or cypermethrin insecticide boards. The level of resistance is determined based on the mosquito mortality rate using WHO standards. The results showed permethrin and cypermethrin resistance in the A. aegypti population of North Denpasar, with mortality of less than 80%.