Study of rust resistance genes in wheat germplasm with DNA markers

Haque, Asma; Shaheen, Tayyaba; Gulzar, Tahsin; Rahman, Mahmood Ur; Jalal, Fatima; Sattar, Summera; Ehsan, Beenish; Iqbal, Zafar; Younas, Muhammad

doi:10.6026/97320630010371

Cited by 9 publications

(6 citation statements)

References 12 publications

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“…Similarly, Yao et al (2006) identified a microsatellite marker Xgwm 526 on the chromosome arm 2BL, linked closely with Yr7 locus resistant to wheat stripe rust. Yr18 gene linked to Xbarc 352 primer was present in 16 RILs at molecular level, similar results were reported by Haque et al (2014) that Xgwm 130 is linked to both of Lr34/Yr18 leaf and stripe rust resistance genes. Yr26 gene linked to Xgwm 11 primer was found to be present in 13 RILs, Yr29 gene was present Table 2.…”

Section: Resultssupporting

confidence: 88%

Molecular analysis of YR genes for yellow rust resistance in bread wheat (Triticum aestivum L. Em Thell)

2019

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Investigation was conducted to evaluate 210 recombinant inbred lines (RILs) of bread wheat to identify Yr genes using SSR markers and response to stripe rust reaction. Recombinant inbred lines were screened under epiphytotic conditions and data in terms of per cent leaf area infected were recorded using Modified Cobb’s Scale. A total of seven Yr genes, namely Yr7, Yr18, Yr26, Yr29, Yr36, Yr47 and Yr53 were found to be linked to yellow rust resistance. Out of 40 Yr specific SSR markers, seven (Xgwm130 (Yr7), Xbarc 352 (Yr18), Xgwm 11 (Yr26), Xwmc 44 (Yr29), Xwmc 149 (Yr53), WKS1_I (Yr36) and Xcfb309 (Yr47) were found to be polymorphic in parental genotypes and RIL population indicating the presence or absence of Yr genes. Three RILs namely, 51, 52, 55 had largest number of resistant genes (4Yr genes) followed by six lines namely, RIL No. 20, 21, 23, 29, 39, 53 had 3Yr genes having disease score (0S). These RILs may be utilized for incorporation of specific Yr genes in well adapted genotypes for improvement of disease resistance in bread wheat.

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Section: Resultssupporting

confidence: 88%

Molecular analysis of YR genes for yellow rust resistance in bread wheat (Triticum aestivum L. Em Thell)

2019

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“…A collection of 54 wheat cultivars and 11 breeding lines from South Africa was screened to identify Sr2 , Sr24 and Sr31 genes using DNA markers [15]. PCR-based DNA markers were used to check rust resistance genes among the 20 wheat genotypes and 22 markers were amplified [16]. Kokhmetova and Atishova [17] studied the presence of Sr genes ( Sr2 , Sr22 , Sr24 , Sr36 , and Sr46 ), which are effective against Ug99 in 88 cultivars of spring soft wheat in Kazakhstan.…”

Section: Introductionmentioning

confidence: 99%

Seedling Resistance to Stem Rust and Molecular Marker Analysis of Resistance Genes in Wheat Cultivars of Yunnan, China

Cao

Wang

et al. 2016

PLoS ONE

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Stem rust is one of the most potentially harmful wheat diseases, but has been effectively controlled in China since 1970s. However, the interest in breeding wheat with durable resistance to stem rust has been renewed with the emergence of Ug99 (TTKSK) virulent to the widely used resistance gene Sr31, and by which the wheat stem rust was controlled for 40 years in wheat production area worldwide. Yunnan Province, located on the Southwest border of China, is one of the main wheat growing regions, playing a pivotal role in the wheat stem rust epidemic in China. This study investigated the levels of resistance in key wheat cultivars (lines) of Yunnan Province. In addition, the existence of Sr25, Sr26, Sr28, Sr31, Sr32, and Sr38 genes in 119 wheat cultivars was assessed using specific DNA markers. The results indicated that 77 (64.7%) tested wheat varieties showed different levels of resistance to all the tested races of Puccinia graminis f. sp. tritici. Using molecular markers, we identified the resistance gene Sr31 in 43 samples; Sr38 in 10 samples; Sr28 in 12 samples, and one sample which was resistant against Ug99 (avirulent to Sr32). No Sr25 or Sr26 (effective against Ug99) was identified in any cultivars tested. Furthermore, 5 out of 119 cultivars tested carried both Sr31 and Sr38 and eight contained both Sr31 and Sr28. The results enable the development of appropriate strategies to breed varieties resistant to stem rust.

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“…Deployment of specific gene combinations provides durable and improved resistance versus using single genes because a single specific gene is subject to become susceptible due to genetic shifts in the pathogen [64]. In our study, nine closely linked markers specific for 5 Yr genes were used to detect the presence of Yr5, Yr10, Yr15, Yr17 and Yr18 genes among the wheat genotypes and evaluate their effect on the resistance to stripe rust.…”

Section: Discussionmentioning

confidence: 99%

“…The 150-bp and 229-bp bands indicated the presence and absence of the Yr18 gene, respectively. Marker analyses indicate that amplification products correspond with the homozygous resistant allele of Lr34/Yr18 gene were detected in 11 wheat entries (Cat# 2, 3,9,13,20,35,54,58,62,64,70), accounting for 15.7% of studied genotypes (Figure S9, Table 1).…”

Section: Identification Of Yr Genes With Molecular Markers and Stripe Rust Resistance In The Sources Of Resistancementioning

confidence: 99%

Identification of Stripe Rust Resistance Genes in Common Wheat Cultivars and Breeding Lines from Kazakhstan

Кохметова

Rsaliyev²,

Malysheva

et al. 2021

Plants

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Stripe (yellow) rust, caused by Puccinia striiformis f.sp. tritici (Pst), is a fungal disease that presents one of the most serious threats to the wheat crops, causing severe yield losses worldwide, including Kazakhstan. The objectives of this study were to: (1) evaluate a winter wheat collection for stripe rust resistance during an adult plant growth stage, (2) identify the presence of selected Yr genes using linked molecular markers in wheat germplasm, (3) identify potentially useful resistant wheat genotypes among leading cultivars and advanced breeding lines. This study evaluated 70 winter wheat genotypes for stripe rust resistance. According to the field reactions, 42 entries (60%) had R or MR reactions including 27 breeding lines (38.6%) and 15 (21.4%) cultivars. Twenty-eight breeding lines/cultivars (40.0%) were susceptible in both years. According to the average coefficient of infection value (ACI) six genotypes were regarded as possessing high level of adult plant resistance. Cultivars/lines carrying Yr10 alone or in combination with other Yr resistance genes provided resistance to stripe rust. Eleven breeding lines showed <5% disease severity in both years. Linked marker analysis revealed the presence of several gene and gene complexes (Yr5, Yr10, Yr15, Yr17/Lr37/Sr38 and Yr18/Lr34). Among a collection of 70 winter wheat breeding lines and cultivars produced in Kazakhstan three stripe rust resistance genes (Yr10, Yr5 and Yr15) demonstrated high frequency occurrence (31.4%, 14.0% and 7.0%, respectively). The most abundant was gene Yr10 identified in 22 genotypes. It was followed by the Yr5 gene, which conferred resistance in 14 lines (20%) and Yr18 gene-11 lines (15.7%). Yr15 was identified in 7 genotypes. Yr17/Lr37/Sr38 gene complex was found in 2 entries. Among 70 evaluated germplasm sources, 42 disease resistant entries are potentially useful resistant wheat genotypes. These carriers of different Yr genes can be used directly in breeding programs to improve stripe rust resistance of winter wheat. Marker-assisted selection can be efficiently applied to develop wheat cultivars with effective gene combinations that would directly assist in developing durable resistance in Kazakhstan.

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Study of rust resistance genes in wheat germplasm with DNA markers

Cited by 9 publications

References 12 publications

Molecular analysis of YR genes for yellow rust resistance in bread wheat (Triticum aestivum L. Em Thell)

Molecular analysis of YR genes for yellow rust resistance in bread wheat (Triticum aestivum L. Em Thell)

Seedling Resistance to Stem Rust and Molecular Marker Analysis of Resistance Genes in Wheat Cultivars of Yunnan, China

Identification of Stripe Rust Resistance Genes in Common Wheat Cultivars and Breeding Lines from Kazakhstan

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