Study of skin and nail Candida species as a normal flora based on age groups in healthy persons in Tehran-Iran

Rafat, Zahra; Hashemi, Seyed Hossein; Ahamdikia, Kazem; Ghazvini, Roshanak Daie; Bazvandi, F.

doi:10.1016/j.mycmed.2017.08.007

Cited by 22 publications

(14 citation statements)

References 14 publications

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“…Skin, comprised of keratinocytes, melanocytes, and fibroblasts, is the largest organ of the human body, and it provides a protective barrier between the internal milieu and the environment ( 9 , 10 ). Keratinocytes are the main cells in the epidermis, which are closely associated with skin diseases such as psoriasis and atopic dermatitis as well as skin wound healing ( 11 – 13 ).…”

Section: Introductionmentioning

confidence: 99%

Low-intensity pulsed ultrasound promotes proliferation and migration of HaCaT keratinocytes through the PI3K/AKT and JNK pathways

Leng

Shang

Gao

et al. 2018

Braz J Med Biol Res

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Although the effects of low-intensity pulsed ultrasound (LIPUS) on diverse cell types have been fully studied, the functional role of LIPUS in keratinocytes remains poorly understood. This study aimed to investigate the effects of LIPUS on proliferation and migration of HaCaT cells as well as the regulatory mechanisms associated with signaling pathways. Human HaCaT cells were exposed or not to LIPUS, and cell proliferation and migration were measured by BrdU incorporation assay and Transwell assay, respectively. Expression of proteins associated with proliferation and migration was evaluated by western blot analysis. Expression of key kinases in the PI3K/AKT and JNK pathways was also evaluated by western blot analysis. Effects of LIPUS on the PI3K/AKT and JNK pathways, and whether LIPUS affected HaCaT cells via these two pathways were finally explored. When the parameter of LIPUS (number of cycles) was set at 300, cell viability was the highest after LIPUS stimulation. We then found that the percentage of BrdU positive cells was enhanced by LIPUS, along with up-regulation of cyclinD1, CDK6, CDK4, and VEGF. LIPUS promoted migration, as well as up-regulation of MMP-2 and MMP-9. Phosphorylation levels of key kinases in the PI3K/AKT and JNK pathways were increased by LIPUS. Inhibition of either PI3K/AKT pathway or JNK pathway attenuated effects of LIPUS on HaCaT cells, and co-inhibition of these two pathways showed augmented effects. LIPUS promoted proliferation and migration of HaCaT cells through activating the PI3K/AKT and JNK pathways.

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Section: Introductionmentioning

confidence: 99%

Low-intensity pulsed ultrasound promotes proliferation and migration of HaCaT keratinocytes through the PI3K/AKT and JNK pathways

Leng

Shang

Gao

et al. 2018

Braz J Med Biol Res

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“…colonization in male participants and 48.7% female participants, with statistically insignificant difference based on sex. 16 Furthermore, Somerville stated that Candida colonization is more common in females than males. 17 Multiple factors, including sociodemographic patterns, economic levels, and lifestyles might affect the research results.…”

Section: Discussionmentioning

confidence: 99%

“…In 2017, Rafat et al reported that Candida colonization found the most in the > 60 years age group (51.2%). 16 The higher prevalence of Candida isolation in elderly might due to a drier skin that corresponds to pH elevation, resulting in a more alkaline skin. This decreases the ability of an impaired immune system in this age group.…”

Section: Discussionmentioning

confidence: 99%

The Comparison of Candida spp. Colonization on Psoriasis Vulgaris Patient and Control

Manuputty

Murtiastutik

Sawitri

et al. 2021

BIKK

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Background: Psoriasis is an inflammatory, chronic, and recurring skin disease that affects patient’s quality of life. Environment, drugs, and infections in the form of antigen and toxin such as Candida spp. are assumed responsible for triggering and exacerbating psoriasis. Purpose: To compare the prevalence of skin colonization of Candida spp. on psoriasis vulgaris patient and control. Methods: This study was observational analytic research involving 33 psoriasis vulgaris patients and 33 healthy individuals. They were matched up based on sex, age, and lesion location. Swab of skin lesions on the Koebner area of both groups was cultured on CHROMagar media. Candida spp. was later identified and the colonization was calculated. Result: Candida spp. was found on 51.5% of lesions in psoriasis vulgaris patients and 21.2% in the control group with a significant difference of p < 0.05. The average colony number of Candida spp. in psoriasis vulgaris group was lower than the control group with no significant difference of p > 0.05. The predominant Candida spp. in the psoriasis vulgaris and control groups was Candida krusei, which were 35.5% and 85.6%, respectively. No significant difference between sex, age, period of complaints, age onset, degree of severity (Psoriasis Area and Severity Index), and therapy history, with positive culture of Candida spp. Conclusion: There was no difference in the number of Candida spp. colonization in the psoriasis vulgaris group and the control group. However, the culture positivity of Candida spp. in the psoriasis vulgaris patients was higher than in the control group.

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“…Despite the predominance of C. albicans in all anatomical sites, Rafat et al (2017) reported a higher frequency of C. parapsilosis than other species in all the groups. The authors did not consider sex as an epidemiological factor of the infection (Rafat et al, 2017). C. albicans is a member of over 700 microbial species estimated to colonize the oral cavity of humans, and this large microbial community occupying the oral niche could be due to neutral saliva pH in addition to other microenvironmental factors (Montelongo-Jauregui and Lopez -Ribot, 2018).…”

Section: Discussionmentioning

confidence: 99%

Proteinase activities of <i>Candida</i> spp. isolated from different anatomical sites of healthy women

Chidebelu¹,

Ogbonna²,

Nweze³

2019

Bio-Research

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Superficial and systemic fungal infections caused by Candida have been increasingly reported in recent times. Hydrolytic enzyme production is an important process in fungal pathogenesis and proteases have been identified as important virulence attributes in Candida species. The aim of the study was to determine and compare the in vitro proteinase activity in sixty Candida spp isolated from three different anatomical sites (vagina, oral cavity and skin) of healthy women. Twenty samples per sample source were collected from apparently healthy female subjects. The recovered Candida isolates were properly identified and screened for proteolytic activity using established procedures. Overall, the recovery rate of Candida albicans was 66.7%, while the non-albicans Candida species represent 25% of the positive samples. Candida albicans recovered from the oral cavity exhibited the highest proteolytic activity (Pz range = 0.41±0.02-0.65 ± 0.04), followed by skin isolates (Pz = 0.50 ± 0.05-0.79 ± 0.06). Isolates from the vagina had the least proteolytic activity (Pz = 0.57 ± 0.03-0.95 ± 0.08). The difference in proteolysis was significant between oral and vagina isolates (p = 0.0042), as well as skin and vaginal isolates (p = 0.0364). This study indicates that C. albicans remains the most prevalent species in all the anatomical body sites investigated. Moreover, the secretion of proteases could prove a potent virulence factor during the pathogenesis of the organism in an otherwise immunocompetent host.

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Study of skin and nail Candida species as a normal flora based on age groups in healthy persons in Tehran-Iran

Cited by 22 publications

References 14 publications

Low-intensity pulsed ultrasound promotes proliferation and migration of HaCaT keratinocytes through the PI3K/AKT and JNK pathways

Low-intensity pulsed ultrasound promotes proliferation and migration of HaCaT keratinocytes through the PI3K/AKT and JNK pathways

The Comparison of Candida spp. Colonization on Psoriasis Vulgaris Patient and Control

Proteinase activities of <i>Candida</i> spp. isolated from different anatomical sites of healthy women

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