Study of spontaneous bioelectrical activity of two hierarchically connected neural networks in vitro

Pigareva, Y.; Grinchuk, Oleg; Gladkov, Arseniy; Колпаков, В. И.; Bukatin, Anton; Kazantsev, Victor B.; Мухина, И.В.; Pimashkin, Alexey

doi:10.1088/1742-6596/1695/1/012198

Cited by 2 publications

(2 citation statements)

References 12 publications

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“…Previously, we showed that the microchannels, which consist of a sequence of triangular shapes, enable unidirectional axon growth between neurons [23]. Increasing the size of the last triangular segment also increased the efficiency of unidirectional axon guidance [30]. The Fish type consisted of three segments: two segments had a narrow triangular shape (200 µm length, 40 µm width), and one triangular segment was larger (200 µm length, 150 µm width) with rounded borders.…”

Section: Microfluidic Device Fabricationmentioning

confidence: 99%

Experimental Platform to Study Spiking Pattern Propagation in Modular Networks In Vitro

et al. 2021

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The structured organization of connectivity in neural networks is associated with highly efficient information propagation and processing in the brain, in contrast with disordered homogeneous network architectures. Using microfluidic methods, we engineered modular networks of cultures using dissociated cells with unidirectional synaptic connections formed by asymmetric microchannels. The complexity of the microchannel geometry defined the strength of the synaptic connectivity and the properties of spiking activity propagation. In this study, we developed an experimental platform to study the effects of synaptic plasticity on a network level with predefined locations of unidirectionally connected cellular assemblies using multisite extracellular electrophysiology.

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Section: Microfluidic Device Fabricationmentioning

confidence: 99%

Experimental Platform to Study Spiking Pattern Propagation in Modular Networks In Vitro

et al. 2021

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“…According to the predefined direction of axon growth, the modules were labeled Source and Target modules. We previously showed that the microchannels with asymmetric shapes provide unidirectional axon growth between neurons [ 50 ]. Here, we used the same type of microchannels that consisted of three segments: two segments had a narrow triangular shape (200 μm length, 40 μm width) and one segment had a large triangular shape (200 μm length, 150 μm width).…”

Section: Methodsmentioning

confidence: 99%

The Profile of Network Spontaneous Activity and Functional Organization Interplay in Hierarchically Connected Modular Neural Networks In Vitro

Pigareva,

Gladkov,

Kolpakov

et al. 2024

Micromachines

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Modern microtechnology methods are widely used to create neural networks on a chip with a connection architecture demonstrating properties of modularity and hierarchy similar to brain networks. Such in vitro networks serve as a valuable model for studying the interplay of functional architecture within modules, their activity, and the effectiveness of inter-module interaction. In this study, we use a two-chamber microfluidic platform to investigate functional connectivity and global activity in hierarchically connected modular neural networks. We found that the strength of functional connections within the module and the profile of network spontaneous activity determine the effectiveness of inter-modular interaction and integration activity in the network. The direction of intermodular activity propagation configures the different densities of inhibitory synapses in the network. The developed microfluidic platform holds the potential to explore function-structure relationships and efficient information processing in two- or multilayer neural networks, in both healthy and pathological states.

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Study of spontaneous bioelectrical activity of two hierarchically connected neural networks in vitro

Cited by 2 publications

References 12 publications

Experimental Platform to Study Spiking Pattern Propagation in Modular Networks In Vitro

Experimental Platform to Study Spiking Pattern Propagation in Modular Networks In Vitro

The Profile of Network Spontaneous Activity and Functional Organization Interplay in Hierarchically Connected Modular Neural Networks In Vitro

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