Study of Sr–Ca–Si-based scaffolds for bone regeneration in osteoporotic models

Wu, Qianju; Wang, Xiao; Jiang, Fei; Zhu, Ziyuan; Wen, Ji; Jiang, Xinquan

doi:10.1038/s41368-020-00094-1

Cited by 25 publications

(13 citation statements)

References 27 publications

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“…From the clinical point of view, new CS-based experimental material has certain advantages since Sr incorporation may enhance the bone healing during root end canal surgery by activating osteoblasts for improved bone synthesis [22,23,24]. In addition, if used for pulp capping procedures, it may stimulate odontoblasts for faster formation of tertiary dentine.…”

Section: Discussionmentioning

confidence: 99%

“…Opšti cilj ove studije bio je da se dodatno poboljša kvalitet cementa ALBO MPCA dodavanjem potencijalno bioaktivnog radiokontrasta -stroncijum-karbonata (SrCO 3 ). Ideja je ukorenjena u dokazima brojnih povoljnih efekata stroncijuma (Sr) na kost i zubna tkiva, među kojima su osteoproliferativni i odontoproliferativni efekati, stimulacija formiranja kosti i angiogeneza, inhibicija ćelijske diferencijacije i osteoklastne aktivnosti, odnosno indukcija humanih stem ćelija zubne pulpe promocijom njihove odontogene diferencijacije, proliferacije i mineralizacije [22,23,24]. Cilj ovog istraživanja je bio da utvrdi minimalni udeo SrCO 3 kao rendgenkontrastnog sredstva koji zadovoljava zahteve ISO u dentalnim cementima i da proveri biokompatibilnost ovog materijala u kulturi ćelija L929.…”

Section: Acknowledgesunclassified

“…Sa kliničke tačke gledišta, novi eksperimentalni materijal na bazi KS može da ima izvesne prednosti budući da dodatak stroncijuma može da poboljša zaceljenje koštanog tkiva nakon periapeksnog hirurškog zahvata aktivirajući osteoblaste [22,23,24]. Takođe, ukoliko se koristi za direktno prekrivanje pulpe, on može da stimuliše osteoblaste u smislu bržeg formiranja tercijarnog dentina.…”

Section: Acknowledgesunclassified

“…This study generally served to further improve the quality of ALBO MPCA cements by incorporating the potentially bioactive radiopacifier -strontium carbonate (SrCO 3 ). The idea is rooted in proofs of numerous beneficial effects of strontium (Sr) on bone and dental tissue, among which are: osteoproliferative and odontoproliferative effects, stimulation of bone formation and angiogenesis, inhibition of cell differentiation and activity of osteoclasts and induction of human dental pulp stem cells by promoting their odontogenic differentiation, proliferation and mineralization [22,23,24]. The aim of this study was to determine the minimal ratio of SrCO 3 capable to satisfy ISO required radiopacity standard and investigate the biocompatibility of this material in L929 cell culture.…”

Section: Introductionmentioning

confidence: 99%

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Investigation of the radiopacity and cytotoxicity of albodent - novel strontium carbonate incorporated calcium silicate based dental cement

et al. 2021

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Introduction. Calcium silicate (CS) dental cements have numerous clinical indications in dentistry including pulp capping, root end surgery, perforation repair and apexification/apexogenesis treatment. Materials and methods. Novel CS based dental cement with incorporation of SrCO3 radiopacifier named ALBO-DENT was used as an experimental cement material while Portland cement (Aalborg, Denmark) and ProRoot MTA (Tulsa Dental, USA) were used as controls. The radiopacity evaluation was performed using digital Trophy Radiographic system with an intention to precisely determine the minimum of radiopaque agent needed to confer to ISO radiopacity requirement. Thereafter, biocompatibility of material was tested in in vitro conditions in mouse fibrosarcoma L929 cell culture treated with materials? extracts. Cell morphology was observed using phase-contrast microscopy, while cell viability was measured using crystal violet (CV) and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide (MTT) assays. Results. Radiopacity evaluation revealed that 30%wt addition of SrCO3 was necessary to achieve satisfactory radiopacity (3.45 mm Al). Cytotoxicity analysis using CV and MTT assays revealed that pure extracts of ALBO-DENT presented superior biocompatibility when compared to PC and MTA controls while serial dilutions of experimental cements? extracts as well as that of PC and MTA did not influence L929 cell viability. Conclusions. Novel formulation of CS cement - ALBO-DENT presented satisfactory radiopacity and adequate biocompatibility.

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Section: Discussionmentioning

confidence: 99%

Section: Acknowledgesunclassified

Section: Introductionmentioning

confidence: 99%

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Investigation of the radiopacity and cytotoxicity of albodent - novel strontium carbonate incorporated calcium silicate based dental cement

et al. 2021

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“…The antimicrobial properties can be further enhanced by appealing to different combinations [ 14 , 15 ]. Other authors [ 16 ] demonstrated the efficiency of Sr incorporation in osteoporotic bone regeneration. Overall, its favorable influences led to the production of materials belonging to different compositional or dimensional classes, including fibers [ 17 ], thin films [ 18 ] and composites [ 19 ].…”

Section: Introductionmentioning

confidence: 99%

Ce/Sm/Sr-Incorporating Ceramic Scaffolds Obtained via Sol-Gel Route

et al. 2021

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Three different inorganic scaffolds were obtained starting from the oxide system SiO2‒P2O5‒CaO‒MgO, to which Ce4+/Sm3+/Sr2+ cations were added in order to propose novel materials with potential application in the field of hard tissue engineering. Knowing the beneficial effects of each element, improved features in terms of mechanical properties, antibacterial activity and cellular response are expected. The compositions were processed in the form of scaffolds by a common sol-gel method, followed by a thermal treatment at 1000 and 1200 °C. The obtained samples were characterized from thermal, compositional, morphological and mechanical point of view. It was shown that each supplementary component triggers the modification of the crystalline phase composition, as well as microstructural details. Moreover, the shrinkage behavior is well correlated with the attained compression strength values. Sm was proven to be the best choice, since in addition to a superior mechanical resistance, a clear beneficial influence on the viability of 3T3 fibroblast cell line was observed.

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Hybrid system with stable structure of hard/soft tissue substitutes induces re‐osseointegration in a rat model of biofilm‐mediated peri‐implantitis

Qin

Mozaffari

et al. 2022

J Biomed Mater Res

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Re‐osseointegration of an infected/contaminated dental implant poses major clinical challenges. We tested the hypothesis that the application of an antibiotic‐releasing construct, combined with hard/soft tissue replacement, increases the efficacy of reconstructive therapy. We initially fabricated semi‐flexible hybrid constructs of β‐TCP/PHBHHx, with tetracycline (TC) (TC amounts: 5%, 10%, and 15%). Thereafter, using in vitro assays, TC release profile, attachment to rat bone marrow‐derived stem cells (rBMSCs) and their viability as well as anti‐bacterial activity were determined. Thereafter, regenerative efficacies of the three hybrid constructs were assessed in a rat model of peri‐implantitis induced by Aggregatibacter actinomycetemcomitans biofilm; control animals received β‐TCP/Bio‐Gide and TC injection. Eight weeks later, maxillae were obtained for radiological, histological, and histomorphometric analyses of peri‐implant tissues. Sulcus bleeding index was chronologically recorded. Serum cytokines levels of IL‐6 and IL‐1β were also evaluated by enzyme‐linked immunosorbent assay. Substantial amounts of tetracycline, from hybrid constructs, were released for 2 weeks. The medium containing the released tetracycline did not affect the adhesion or viability of rBMSCs; however, it inhibited the proliferation of A. actinomycetemcomitans. Osteogenesis and osseointegration were more marked for the 15% hybrid construct group than the other two groups. The height of attachment and infiltration of inflammatory cells within fibrous tissue was significantly reduced in the experimental groups than the control group. Our protocol resulted in re‐osseointegration on a biofilm‐contaminated implant. Thus, an antibiotic releasing inorganic/organic construct may offer a therapeutic option to suppress infection and promote guided tissue regeneration thereby serving as an integrated multi‐layer substitute for both hard/soft tissues.

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Study of Sr–Ca–Si-based scaffolds for bone regeneration in osteoporotic models

Cited by 25 publications

References 27 publications

Investigation of the radiopacity and cytotoxicity of albodent - novel strontium carbonate incorporated calcium silicate based dental cement

Investigation of the radiopacity and cytotoxicity of albodent - novel strontium carbonate incorporated calcium silicate based dental cement

Ce/Sm/Sr-Incorporating Ceramic Scaffolds Obtained via Sol-Gel Route

Hybrid system with stable structure of hard/soft tissue substitutes induces re‐osseointegration in a rat model of biofilm‐mediated peri‐implantitis

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