Study of Steinernema hermaphroditum (Nematoda, Rhabditida), from the West Uttar Pradesh, India

Description of Heterorhabditis bacteriophora (Nematoda: Heterorhabditidae) isolated from hilly areas of Kashmir Valley

“…Geographical distribution of H. bacteriophora was assessed, using a meta-analysis of GenBank records, as natural occurrence of species facilities is use in particular areas. The ITS sequence was selected for the analysis, as it enables a clear distinction of the species in steinernematid, unlike another frequently sequenced marker D2D3 region of the 28S rDNA (Bhat et al, 2019). For determining the sequences of H. bacteriophora, BLAST search was performed with the sequence of the type isolate (AY321477) as a search query.…”

“…Though, research on EPNs in India was initiated since the mid-1960s (Kaya et al, 2006), but exploration of indigenous EPNs strain started in 1990s (Sankaranarayanan and Askary, 2017) with the isolation and identification of H. indica (Poinar et al, 1992) from Coimbatore. In India, H. indica is the only new valid species of EPNs reported till date; however, previously described new species were either synonymized with already existing species or were included as species inquirendae (Bhat et al, 2019;Hunt and Subbortin, 2016).…”

Morphological and molecular characterization of Acrobeloides saeedi Siddiqi, De Ley and Khan, 1992 (Rhabditida, Cephalobidae) from India and comments on its status

“…The cadavers were transferred to white trap ( White, 1927 ) after proper washing with double distilled water and sterilization with 1% NaOCl. The nematodes that emerge in white trap were harvested, and stored in 250 ml tissue culture flasks in incubator at 15°C as described by Bhat et al (2019) . For observations and morphometrics, third-stage juveniles (200) were injected to larvae of Galleria mellonella by Insulin Syringe 1 ml and larvae were killed within 36 hr at 27°C.…”

Morphological, morphometrical, and molecular characterization of Metarhabditis amsactae (Ali, Pervez, Andrabi, Sharma and Verma, 2011) Sudhaus, 2011 (Rhabditida, Rhabditidae) from India and proposal of Metarhabditis longicaudata as a junior synonym of M. amsactae

“…The PCR profiles used were 1 cycle of 94°C for 3 min followed by 40 cycles of 94°C for 30 sec, 52°C for 30 sec for LSU (28S) rDNA or 55°C for 30 sec for ITS rDNA or 54°C for 30 sec for SSU (18S) rDNA, 72°C for 60 sec, and a final extension at 72°C for 10 min. PCR was followed by electrophoresis (45 min, 100 volts) of 5 μl of PCR product in a 1% TAE (Tris–acetic acid–EDTA) buffered agarose gel stained with ethidium bromide ( Bhat et al, 2020c ; Rana et al, 2020a ). The amplified products were purified and Sanger sequenced in both directions by Bioserve Technologies Ltd. (Hyderabad, India).…”