Study of steroidogenesis in pupae of the forensically important blow fly Protophormia terraenovae (Robineau-Desvoidy) (Diptera: Calliphoridae)

Gaudry, Emmanuel; Blais, Catherine; Maria, Annick; Dauphin‐Villemant, Chantal

doi:10.1016/j.forsciint.2005.06.014

Cited by 19 publications

(9 citation statements)

References 21 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…By virtue of the constant interest in establishing more and more precise methods to determine the age of juvenile blow flies, i.e., blow fly pupae, using gene expression was focused in recent years [ 21 , 22 , 23 , 24 , 25 , 26 , 35 ]. However, these approaches were mainly hampered by high interspecific variability compared with age specific differences.…”

Section: Resultsmentioning

confidence: 99%

Dating Pupae of the Blow Fly Calliphora vicina Robineau–Desvoidy 1830 (Diptera: Calliphoridae) for Post Mortem Interval—Estimation: Validation of Molecular Age Markers

Zajac

Amendt

Verhoff

et al. 2018

Genes

View full text Add to dashboard Cite

Determining the age of juvenile blow flies is one of the key tasks of forensic entomology when providing evidence for the minimum post mortem interval. While the age determination of blow fly larvae is well established using morphological parameters, the current study focuses on molecular methods for estimating the age of blow flies during the metamorphosis in the pupal stage, which lasts about half the total juvenile development. It has already been demonstrated in several studies that the intraspecific variance in expression of so far used genes in blow flies is often too high to assign a certain expression level to a distinct age, leading to an inaccurate prediction. To overcome this problem, we previously identified new markers, which show a very sharp age dependent expression course during pupal development of the forensically-important blow fly Calliphora vicina Robineau–Desvoidy 1830 (Diptera: Calliphoridae) by analyzing massive parallel sequencing (MPS) generated transcriptome data. We initially designed and validated two quantitative polymerase chain reaction (qPCR) assays for each of 15 defined pupal ages representing a daily progress during the total pupal development if grown at 17 °C. We also investigated whether the performance of these assays is affected by the ambient temperature, when rearing pupae of C. vicina at three different constant temperatures—namely 17 °C, 20 °C and 25 °C. A temperature dependency of the performance could not be observed, except for one marker. Hence, for each of the defined development landmarks, we can present gene expression profiles of one to two markers defining the mentioned progress in development.

show abstract

Section: Resultsmentioning

confidence: 99%

Dating Pupae of the Blow Fly Calliphora vicina Robineau–Desvoidy 1830 (Diptera: Calliphoridae) for Post Mortem Interval—Estimation: Validation of Molecular Age Markers

Zajac

Amendt

Verhoff

et al. 2018

Genes

View full text Add to dashboard Cite

show abstract

“…Boehme et al [20,41], Baqué et al [38], and Tarone and Foran [21] have recently shown that the analysis of gene expression patterns can assist in age determination in blow flies [20,21,38,41]. Due to a lack of blow fly sequence data and low homology between blow flies and the model-organism Drosophila melanogaster, or even between the two blow fly species L. sericata and C. vicina, identifying suitable markers has so far been associated with time-consuming preliminary investigations, such as gene fishing [20,21,23,39,40].…”

Section: Discussionmentioning

confidence: 99%

De novo transcriptome analysis and highly sensitive digital gene expression profiling of Calliphora vicina (Diptera: Calliphoridae) pupae using MACE (Massive Analysis of cDNA Ends)

Zajac¹,

Amendt²,

Horres³

et al. 2015

Forensic Science International: Genetics

View full text Add to dashboard Cite

“…Enzyme Immunoassay (EIA) of Ecdysteroids EIA was performed using an polyclonal antiserum (AS 4919) provided by Prof Patrick Porcheron (Paris) and a laboratory-made enzymatic tracer (2-succinyl-20-hydroxyecdysone coupled to peroxidase as previously described (Gaudry et al, 2006).…”

Section: Spectroscopic Methodsmentioning

confidence: 99%

Ecdysteroids from Cyanotis longifolia Benth. (Commelinaceae)

Crouzet¹,

Maria²,

Dinan³

et al. 2009

Arch Insect Biochem Physiol

Self Cite

View full text Add to dashboard Cite

Cyanotis longifolia Benth. (Commelinaceae) contains ecdysteroids, which are highly concentrated in the roots and flowers, whereas leaves contain only very low amounts and stems intermediate amounts. 20-Hydroxyecdysone is the major component found in all tissues, but roots also contain large amounts of 20-hydroxyecdysone 3-acetate and ajugasterone C. A preparative experiment has shown that roots contain a complex ecdysteroid mixture, and the analysis of minor components has allowed the isolation of several already known ecdysteroids (polypodine B, 2-deoxy-20,26-dihydroxyecdysone, isovitexirone, poststerone) together with five new (ajugasterone C 3-acetate, 5beta-hydroxy-poststerone, poststerone 2-acetate, 14(15)-dehydro-poststerone 2-acetate, 24-epi-atrotosterone A [=24-methyl-ajugasterone C]) ecdysteroids that have been fully characterized. A preliminary investigation of 55 species belonging to 15 different genera of the Commelinaceae has shown that several of them contain significant concentrations of ecdysteroids, among which some previously uninvestigated ones appear to be very promising sources of ecdysteroids.

show abstract

Study of steroidogenesis in pupae of the forensically important blow fly Protophormia terraenovae (Robineau-Desvoidy) (Diptera: Calliphoridae)

Cited by 19 publications

References 21 publications

Dating Pupae of the Blow Fly Calliphora vicina Robineau–Desvoidy 1830 (Diptera: Calliphoridae) for Post Mortem Interval—Estimation: Validation of Molecular Age Markers

Dating Pupae of the Blow Fly Calliphora vicina Robineau–Desvoidy 1830 (Diptera: Calliphoridae) for Post Mortem Interval—Estimation: Validation of Molecular Age Markers

De novo transcriptome analysis and highly sensitive digital gene expression profiling of Calliphora vicina (Diptera: Calliphoridae) pupae using MACE (Massive Analysis of cDNA Ends)

Ecdysteroids from Cyanotis longifolia Benth. (Commelinaceae)

Contact Info

Product

Resources

About