Study of the aggregation of bovine serum albumin monomers in aqueous dispersions with different acid-base value pH

Kvasnov, Bogdan A.; Shalaev, Pavel V.; Dolgushin, S. A.

doi:10.1109/eiconrus.2018.8317480

Cited by 4 publications

(2 citation statements)

References 5 publications

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“…In the basic medium, amine groups can transfer electrons to the core fluorescent units, thus decreasing the overall PL intensity, whereas protonation of the amine groups under acidic conditions eliminates the electron transfer leading to higher PL intensity. Trends in hydrodynamic diameter of the conjugates were generally similar to the trend of BSA in different pH media as reported by Kvasnov et al Collectively, these results indicate that the stabilities of noncovalent TP–BSA conjugates were highly similar to that of covalent TP–BSA at physiological pH, suggesting that the linker-free conjugation method is an effective way to develop fluorescent-labeled protein probes for biosensing applications.…”

Section: Results and Discussionsupporting

confidence: 85%

Noncovalent Fluorescent Biodot–Protein Conjugates with Well-Preserved Native Functions for Improved Sweat Glucose Detection

et al. 2020

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To overcome the traditional issues of protein labeling, we report herein an effective approach for noncovalent conjugation of the biomolecule-derived fluorescent nanodots (biodot) to functional proteins without the addition of chemical linkers for biosensor development. The as-prepared fluorescent biodot–protein conjugates are very stable near physiological pH, exhibiting excellent photostability and thermal stability. More importantly, the native functions of proteins, including drug binding and enzymatic activities, are well-preserved after conjugating with biodots. The optimized protein conjugation strategy is then applied to prepare biodot-glucose oxidase (GOx) fluorescent sensing probes for sweat glucose detection. Results show that the as-prepared sensing probes could achieve better assay performance than those covalent conjugates as demonstrated herein. Specifically, GOx in the noncovalently bound conjugates are able to catalyze the oxidation of glucose effectively, which generates hydrogen peroxide as a byproduct. In the presence of Fe2+, Fenton reaction takes place to produce hydroxyl radicals and Fe3+, leading to significant fluorescence quenching of biodots on the conjugates. This simple one-step enzymatic assay in a single probe achieves a wide linear range of 25–1000 μM (R 2 = 0.99) with a low detection limit of 25 μM. Furthermore, negligible interference is observed in the complex artificial sweat sample for accurate glucose quantification, achieving an excellent recovery rate of 100.5 ± 2.2%. This work provides a facile conjugation method that is generally applicable to a wide range of proteins, which will help to accelerate future development of multifunctional fluorescent probes to provide optical signals with unique protein functions (e.g., enzymatic, recognition, etc.) for biomedical sensing and imaging.

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Section: Results and Discussionsupporting

confidence: 85%

Noncovalent Fluorescent Biodot–Protein Conjugates with Well-Preserved Native Functions for Improved Sweat Glucose Detection

et al. 2020

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“…Later, the same group were able to produce protein aggregates of the desired size using bovine serum albumin, a protein with a great tendency to aggregate [324][325][326][327]; just decreasing the activation of the support, it was possible to get larger aggregates, being the dexOx crosslinking essential to maintain these aggregates after their release from the support [328] (Figure 24). Later, the same group were able to produce protein aggregates of the desired size using bovine serum albumin, a protein with a great tendency to aggregate [324][325][326][327]; just decreasing the activation of the support, it was possible to get larger aggregates, being the dexOx crosslinking essential to maintain these aggregates after their release from the support [328] (Figure 24).…”

Section: -Use Of Dexox For Intermolecular Crosslinking Of Immobilizedmentioning

confidence: 99%

Dextran Aldehyde in Biocatalysis: More Than a Mere Immobilization System

et al. 2019

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Dextran aldehyde (dexOx), resulting from the periodate oxidative cleavage of 1,2-diol moiety inside dextran, is a polymer that is very useful in many areas, including as a macromolecular carrier for drug delivery and other biomedical applications. In particular, it has been widely used for chemical engineering of enzymes, with the aim of designing better biocatalysts that possess improved catalytic properties, making them more stable and/or active for different catalytic reactions. This polymer possesses a very flexible hydrophilic structure, which becomes inert after chemical reduction; therefore, dexOx comes to be highly versatile in a biocatalyst design. This paper presents an overview of the multiple applications of dexOx in applied biocatalysis, e.g., to modulate the adsorption of biomolecules on carrier surfaces in affinity chromatography and biosensors design, to serve as a spacer arm between a ligand and the support in biomacromolecule immobilization procedures or to generate artificial microenvironments around the enzyme molecules or to stabilize multimeric enzymes by intersubunit crosslinking, among many other applications.

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On approximation and experimental accuracy in dynamic light scattering

Nepomnyashchaya

2019

J. Phys.: Conf. Ser.

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The work is dedicated to estimation of accuracy of calculations in dynamic light scattering experiments, in particular approximation and size determination of nanoparticles in polydisperse solutions. In dynamic light scattering the proper approximation is crucial to final accuracy of sizes calculations, especially if we consider polydisperse solutions, such as biological fluids. Calculated and experimental errors have been compared.

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Study of the aggregation of bovine serum albumin monomers in aqueous dispersions with different acid-base value pH

Cited by 4 publications

References 5 publications

Noncovalent Fluorescent Biodot–Protein Conjugates with Well-Preserved Native Functions for Improved Sweat Glucose Detection

Noncovalent Fluorescent Biodot–Protein Conjugates with Well-Preserved Native Functions for Improved Sweat Glucose Detection

Dextran Aldehyde in Biocatalysis: More Than a Mere Immobilization System

On approximation and experimental accuracy in dynamic light scattering

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