Study of the Biosynthesis of 1‐Octen‐3‐ol Using a Crude Homogenate of Agaricus bisporus in a Bioreactor

Abstract

1-Octen-3-ol and 10-oxo-trans-8-decenoic acid are metabolites of the breakdown of linoleic acid (LA) by mushroom enzymes. These compounds can be produced in a bioreactor using a crude mushroom homogenate and the exogenous addition of LA and oxygen. The factors' duration of blending, mushroom-buffer ratio, effect of a surfactant, whole against partially clarified reaction broths, purity of LA, and utilization of stumps instead of whole mushrooms were studied for their effect on reaction yield using a 1-L bioreactor. The results showed the feasibility of using the more inexpensive 60%-pure LA instead of the 99%-pure LA even when a yield loss was involved. Waste stumps could be used instead of whole mushrooms with a yield decline of 26%.

Chemosensory adaptations of the mountain fly Drosophila nigrosparsa (Insecta: Diptera) through genomics’ and structural biology’s lenses

“…Modifications in DnigOr56a receptor may thus possibly reflect important adaptation towards different types of toxic compounds possibly present in fungi, and their recognition in non-suitable fungi species might inhibit positive chemotaxis, avoiding the oviposition 40 . Given that fungi are known to produce various volatile substances such as 1-octen-3-ol (called mushroom alcohol) 56 , DnigOr56a receptor modifications may alternatively be key to identifying suitable oviposition substrate using substances produced by fungi. This scenario would be in line with findings for Drosophila specialised on yeast 57 , such as D. melanogaster, D. mojavensis , and D. suzukii 58 59 .…”

Evaluation of aroma active compounds in Tuber fruiting bodies by gas chromatography–olfactometry in combination with aroma reconstitution and omission test

“…2c). In addition, 1-octen-3-ol could be produced from the metabolism of linoleic acid (Morawicki and Beelman 2008). 3-Methylbutanal could be derived by the metabolic pathways of L-leucine (Smit et al 2004).…”

Bio‐Based Cycloalkanes: The Missing Link to High‐Performance Sustainable Jet Fuels

“…[336] Linoleic acid is a primary component of safflower, sunflower, grapeseed, corn, and soybean oil that can be oxidatively cleaved by mushroom enzymes to generate primarily 1octen-3-ol and 10-oxo-8-decenoic acid (ODA). [337][338][339][340] Dehydration of the alcohol with p-toluenesulfonic acid (PTSA) produced a mixture of 1,3-and 2,4-octadienes. Solvent and catalyst-free Diels-Alder [4 + 2]-cycloaddition then allowed for 90 % conversion of the octadienes and a dimer/trimer ratio of 7.8.…”

“…In addition to C 5 and C 6 alkenes, Harvey and co‐workers recently demonstrated the use of bio‐based octadienes as substrates for the preparation of high density cyclic fuels [336] . Linoleic acid is a primary component of safflower, sunflower, grapeseed, corn, and soybean oil that can be oxidatively cleaved by mushroom enzymes to generate primarily 1‐octen‐3‐ol and 10‐oxo‐8‐decenoic acid (ODA) [337–340] . Dehydration of the alcohol with p ‐toluenesulfonic acid (PTSA) produced a mixture of 1,3‐ and 2,4‐octadienes.…”