Study of the carotenoid pigments of Bixa orellana L. Seeds by thin layer chromatography

Tirimanna, A. S. L.

doi:10.1007/bf01197299

Cited by 34 publications

(10 citation statements)

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“…Tirimanna 59 identified other xanthophylls dyes in the seeds of annatto such as lutein (Figure 1c) and zeaxanthin, in which 13 C NMR spectra show peaks that could be assigned to the carbon atoms of the cyclohexane ring, as follow: 58,60 CH 3 (20-30 ppm), carbon bounded to methyl groups (around 35 ppm), > CH 2 (42-48 ppm) and C-OH (65.0 ppm). In other study, Costa and Chaves 61 reported the presence of bixin and geranylgeraniol in the extract obtained treating annatto seeds with a solution of 5% NaOH.…”

Section: Resultsmentioning

confidence: 99%

Intercalation of Apocarotenoids from Annatto (Bixa orellana L.) into Layered Double Hydroxides

Perotti¹,

Silva²,

Couto³

et al. 2020

J. Braz. Chem. Soc.

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Synthetic conditions were evaluated to intercalate norbixin into layered double hydroxides (LDH). Norbixin was isolated from a hydrosoluble commercial annatto extract. LDH samples comprising M 2+ /Al 3+ (M = Mg, Zn) were synthesized by coprecipitation and characterized by X-ray diffraction, thermal analysis coupled to mass spectrometry, vibrational spectroscopies, and solid-state 13 C nuclear magnetic resonance. Radical scavenging activity of LDH hybrids was monitored by electron paramagnetic resonance towards the stable radical DPPH (1,1-diphenyl-2-picrylhydrazyl). LDH hosts presented more than 50 wt.% of norbixin and d-spacing (003) values of about 2.5 or 3.0 nm depending of the cation LDH composition. Vibrational spectra assignment (supported by density functional theory calculations) confirmed the chemical integrity of norbixin after intercalation into LDH. The inorganic layers were found to enhance the thermal stability of norbixin upon intercalation. The free radical scavenging capacity of norbixin was retained when immobilized into LDH. Considering that zinc and magnesium-LDH matrices are biocompatible and that norbixin presents biological activity, the hybrid materials appear as promising systems for biomedical assessment.

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Section: Resultsmentioning

confidence: 99%

Intercalation of Apocarotenoids from Annatto (Bixa orellana L.) into Layered Double Hydroxides

Perotti¹,

Silva²,

Couto³

et al. 2020

J. Braz. Chem. Soc.

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“…Differently, β-lcy mRNA expression coincided with the accumulation of carotenoids in the pink cultivar. Thus, the expression of the β-lcy gene is probably related to the biosynthesis of β, β′-xanthophylls, which are accumulated in the colored petals of the pink variant and with the production of β-apocarotenoids such as ABA and other β-carotene derivates not analyzed here (i.e., methyl (all-E)-8′-apo-β-caroten-8′-oate), which have previously been reported in mature seeds of B. orellana (Bittencourt et al 2005b;Tirimanna 1981).…”

Section: Pigment-related Gene Expression Profilesmentioning

confidence: 85%

Regulation of Pigment-Related Genes During Flower and Fruit Development of Bixa orellana

et al. 2010

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Annatto (Bixa orellana) is the sole source of the commercially important pigment bixin. The broad diversity of annatto phenotypes and the wide variation in pigment contents have restricted its use. Therefore, genetic improvement focusing on this variation is important in order to achieve optimal exploitation of annatto. To characterize and understand some of the genetic basis governing carotenoid production, two annatto cultivars with either pink or white flowers were selected on the basis of their contrasting pigment levels. Several complementary DNA (cDNA)-probes corresponding to genes involved in carotenoid biosynthesis (dxs, psy, pds, β-lcy, and ε-lcy) were cloned and analyzed by reverse transcriptase-polymerase chain reaction (RT-PCR) in different tissues of these two annatto cultivars during flower and fruit development. In general, expression of most selected genes could be associated with pigment overaccumulation in these cultivars. The pink cultivar exhibited higher carotenoid contents and higher pigment-related gene expression, and the two variants analyzed showed notable differences in gene expression in the highest pigment producing tissues.

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“…The solvent systems used for TLC development of supercritical fluid extracts was toluene and for organic extracts toluene/acetone (1:l). The most common method for analysis of annatto pigments is normal phase TLC, for which several solvent systems have been used (Francis, 1965;Ramamurthy and Bhalerao, 1964;Trimanna, 1981).…”

Section: Methodsmentioning

confidence: 99%

Supercritical CO₂ Extraction of Annatto (Bixa orellana) Pigments and Some Characteristics of the Color Extracts

Chao

Mulvaney

Sanson

et al. 1991

Journal of Food Science

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Supercritical CO* (SC-CO*) was used to extract natural food colors from Annatto (Siua orellana) seeds with different pressures and temperatures. Results from the reverse thin-layer chromatographic separation and GUMS revealed that SC-CO2 extracts were composed mainly of bixin, geranylgeraniol and norbixin. Differences in absolute values of solubility parameters (SPD) between these main compounds and SC-CO2 indicated bixin was more easily extracted than norbixin under conditions used. A higher yield of total pigments was obtained at extraction pressure above 310 bar than at 210 bar and 50°C.

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Study of the carotenoid pigments of Bixa orellana L. Seeds by thin layer chromatography

Cited by 34 publications

References 1 publication

Intercalation of Apocarotenoids from Annatto (Bixa orellana L.) into Layered Double Hydroxides

Intercalation of Apocarotenoids from Annatto (Bixa orellana L.) into Layered Double Hydroxides

Regulation of Pigment-Related Genes During Flower and Fruit Development of Bixa orellana

Supercritical CO₂ Extraction of Annatto (Bixa orellana) Pigments and Some Characteristics of the Color Extracts

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Study of the carotenoid pigments of Bixa orellana L. Seeds by thin layer chromatography

Cited by 34 publications

References 1 publication

Intercalation of Apocarotenoids from Annatto (Bixa orellana L.) into Layered Double Hydroxides

Intercalation of Apocarotenoids from Annatto (Bixa orellana L.) into Layered Double Hydroxides

Regulation of Pigment-Related Genes During Flower and Fruit Development of Bixa orellana

Supercritical CO2 Extraction of Annatto (Bixa orellana) Pigments and Some Characteristics of the Color Extracts

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Supercritical CO₂ Extraction of Annatto (Bixa orellana) Pigments and Some Characteristics of the Color Extracts