Study of the combined treatment of lung cancer using gene-loaded immunomagnetic albumin nanospheres in vitro and in vivo

Abstract

Combination therapy for lung cancer has garnered widespread attention. Radiation therapy, gene therapy, and molecular targeted therapy for lung cancer have certain effects, but the disadvantages of these treatment methods are evident. Combining these methods can decrease their side effects and increase their curative effects. In this study, we constructed a pYr-ads-8-5HRE-cfosp-iNOS-IFNG plasmid (a gene circuit that can express IFNγ), which is a gene circuit, and used that plasmid together with C225 (cetuximab) to prepare gene-loaded immunomagnetic albumin nanospheres (IMANS). Moreover, we investigated the therapeutic effects of gene-loaded IMANS in combination with radiation therapy on human lung cancer in vitro and in vivo. The results showed that this gene circuit was successively constructed and confirmed that the expression of INFγ was increased due to the gene circuit. Gene-loaded IMANS combined with radiation therapy demonstrated improved results in vitro and in vivo. In conclusion, gene-loaded IMANS enhanced the efficacy of combination therapy, solved problems related to gene transfer, and specifically targeted lung cancer cells.

Targeted CDX2 expression inhibits aggressive phenotypes of colon cancer cells in vitro and in vivo

“…Herein, we used 5 copies of the hypoxia responsive element (HRE) as an hTERT promoter enhancer (5HRE). The 5HRE element has previously been used as an enhancer to utilize the hypoxic microenvironment ( 39 ). Harvey et al ( 40 ) developed a hypoxia-targeted gene therapy strategy using the herpes simplex virus thymidine kinase and bacterial nitroreductase pro-drug-activating genes and showed that 5HRE linked to the CMV minimal promoter could induce optimum luciferase reporter gene expression.…”

Albumin Nanovectors in Cancer Therapy and Imaging

“…Albumin coating increased the yield of antisense oligonucleotides and siRNA transfection when used to coat positively charged liposomes [141] while decreasing their toxic effects. Similarly, Zhang et al [142] combined PEI-Fe3O4 magnetic nanoparticles with albumin via standard desolvation/cross-linking protocols to increase their biocompatibility, delivering more efficiently a plasmid to overexpress Interferon-γ in cancer cells. Figure 6 is a schematic representing the advantages of a surface functionalization based on albumin.…”

Hypoxia Response Element-Directed Expression of aFGF in Neural Stem Cells Promotes the Recovery of Spinal Cord Injury and Attenuates SCI-Induced Apoptosis

“…Emulated with adenovirus and lentivirus, AAV is rendered with more stable expression ability and higher immunocompatibility in nerve cells (Li and Samulski, 2020). The HRE can induce the expression of specific genes under hypoxic conditions (Schörg et al, 2015;Zhang et al, 2016). We used AAV to carry 5HRE-aFGF into NSCs and achieved a high expression of aFGF induced by hypoxia.…”