Study of the Complex between RNA and DEAE-Dextran

Synthesis and characterization of 2‐diethyl‐aminoethyl–dextran–methyl methacrylate graft copolymer for nonviral gene delivery vector

“…DEAE–dextran has strong adsorbing properties with nucleic acids, such as DNA and RNA, because of its cationic properties and is able to adsorb specific nucleic acids by changing the pH and ion strength 8, 9…”

Mechanism of Introduction of Exogenous Genes into Cultured Cells Using DEAE-Dextran-MMA Graft Copolymer as Non-Viral Gene Carrier

“…We found that 48 h is the optimal condition for DEAE-dextran. However, with the complex formation reaction for DEAE-dextran and DNA, Coulomb forces are well understood to be the primary factor in the Poly-ion complex (PIC) reaction, and thus experiments were carried out to compare the DDMC complex formation reaction with the case of DEAE-dextran [3,4]. …”

“…Although these compounds have problems with cytotoxicity and low transformation rates, they have a history of use as nonviral vectors, and desirable DEAE-dextrans (2-diethylaminoethyl-dextran) are currently being closely investigated because they can be sterilized [3,4]. Copolymers formed by graft polymerization of methyl methacrylate (MMA) with DEAE-dextran have hydrophilic and hydrophobic regions and are known to be desirable non-viral vectors due to their high transformation efficiency [5,6,7].…”

Mechanism of the Introduction of Exogenous Genes into Cultured Cells Using DEAE-Dextran-MMA Graft Copolymer as a Non-Viral Gene Carrier. II. Its Thixotropy Property

“…In the complex formation reaction that occurs between DEAEdextran and DNA, Coulomb forces are understood to be the primary factor in the poly-ion complex (PIC) reaction, and thus experiments were carried out to compare the DDMC-DNA complex formation reaction with that of DEAE-dextran [3,4]. The DDMC-DNA complex formation reaction should be different from that of DEAE-dextran because DDMC possesses a hydrophilic-hydrophobic micro-separated domain.…”

“…Cationic polymers are man-made materials and are thus expected to be stable at high temperatures [2]. These compounds have some problems with their cytotoxicity and low transformation rates; however, they have a long history of use as non-viral vectors, and DEAE-dextrans (2-diethylaminoethyl-dextran) are currently being closely investigated because they can be sterilized by autoclaving [3,4]. directly proportional to the transformation rate, but the complex formation reaction, which is driven by the Coulomb forces between DDMC and DNA, is also significantly influenced by hydrophobic bonding strength as well as hydrogen bonding strength due to the hydrophobicity of the grafted MMA sections.…”