Study of the CTLA-4 gene polymorphisms in systemic lupus erythematosus (SLE) samples from Malaysia

Chua, Kek Heng; Puah, Suat Moi; Chew, C. H.; Tan, Si-Yen; Lian, Lay-Hoong

doi:10.3109/03014460903325185

Cited by 34 publications

(27 citation statements)

References 15 publications

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“…All amplifications were performed in a final volume of 20 mL containing 100 ng genomic DNA and appropriate PCR mixture, as previously described (Chua et al, 2009). Following that, PCR was performed in a thermal cycler according to the conditions described by Chua et al (2010).…”

Section: Genotypingmentioning

confidence: 99%

Lack of association between Gly82Ser, 1704G/T and 2184A/G of RAGE gene polymorphisms and retinopathy susceptibility in Malaysian diabetic patients

Ng¹,

Kuppusamy²,

Poh³

et al. 2012

Genet. Mol. Res.

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ABSTRACT. Diabetic retinopathy is the most common diabetic eye disease, occurring in about 60% of type 2 diabetic patients. Other than known clinical risk factors, the influence of genes has been suggested as part of the development of diabetic retinopathy. We investigated the association of Gly82Ser, 1704G/T and 2184A/G polymorphisms in the RAGE gene with retinopathy in type 2 diabetic patients in Malaysia. Ninety-eight unrelated retinopathy patients and 185 unrelated healthy controls from all over Malaysia were recruited in this study. The allele and genotype frequencies of the three gene polymorphisms were investigated using PCR-RFLP. The allele frequency of the three polymorphisms did not differ significantly between the control and the retinopathy group (P > 0.05). Analysis of the frequency of GA+AA, GT+TT and AG+GG in the retinopathy group did not reveal significant differences (P > 0.05) compared to the control group. We conclude that RAGE gene Gly82Ser, 1704G/T and 2184A/G polymorphisms are not associated with retinopathy development in the Malaysian population.

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Section: Genotypingmentioning

confidence: 99%

Lack of association between Gly82Ser, 1704G/T and 2184A/G of RAGE gene polymorphisms and retinopathy susceptibility in Malaysian diabetic patients

Ng¹,

Kuppusamy²,

Poh³

et al. 2012

Genet. Mol. Res.

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“…ECP is a specific marker of eosinophils (Ursaciuc et al, 2010;Shah et al, 2012;Beigelman, 2014;Smith et al, 2015), which is mainly activated and released by eosinophils and causes neurotoxicity and cytotoxicity. In addition, ECP induces release of histamine by mast cells, causing airway hyperresponsiveness, resulting in bronchial asthma attack (Reed et al, 2007;Choi et al, 2010;Chua et al, 2010;Ursaciuc et al, 2010;Wei et al, 2010;Braga et al, 2011;Olive et al, 2011).…”

Section: Discussionmentioning

confidence: 99%

Eosinophil cationic protein mRNA expression in children with bronchial asthma

Yu¹,

Li²,

Zf³

et al. 2015

Genet. Mol. Res.

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ABSTRACT. Studies have shown that eosinophils are closely related to pathogenesis of bronchial asthma. Eosinophils release eosinophil cationic protein (ECP), which plays an important role in infection and allergic reactions. Serum ECP mRNA expression in children with bronchial asthma has not been adequately investigated. We analyzed serum ECP mRNA expression in 63 children with bronchial asthma and 21 healthy children by using reverse-transcriptase polymerase chain reaction to understand the role of ECP in children with bronchial asthma. The children with bronchial asthma were segregated into acutephase and stable-phase groups, based on the severity of the illness. Serum ECP mRNA expression in children with bronchial asthma (0.375 ± 0.04) was significantly higher than that in healthy controls (0.20 ± 0.02; P < 0.05). Additionally, children in the acute-phase group showed higher ECP mRNA expression level (0.44 ± 0.06) than those in the stablephase (0.31 ± 0.03) and healthy control groups (0.20 ± 0.02; P < 0.05), while the level in the stable-phase (0.31 ± 0.03) was markedly higher than that in the healthy control group (0.20 ± 0.02; P < 0.05). Detection of serum ECP mRNA expression level has possible applications in the diagnosis and treatment of children with bronchial asthma.

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“…Two polymorphic sites { + 49A/ G (rs231775) and CT60A/G (rs3087243)} were identified and used for the meta-analysis. Finally, 15 references were included in this research (Heward et al, 1999;Matsushita et al, 1999;Pullmann et al, 1999;D'Alfonso et al, 2000;Ahmed et al, 2001;Lee et al, 2001;Liu et al, 2001;Hudson et al, 2002;Aguilar et al, 2003;Barreto et al, 2004;Parks et al, 2004;Torres et al, 2004;Ulker et al, 2009;Chua et al, 2010;Kimkong et al, 2011). We subdivided the group of + 49A/G (rs231775) into Asia subgroup and Europe subgroup (five studies about Europe and eight studies about Asia).…”

Section: Ctla-4 Snp and Slementioning

confidence: 99%

“…The + 49A/G (rs231775) SNP causes an amino acid change from threonine to alanine in the peptide leader sequence of the CTLA-4 protein (Chistiakov and Turakulov, 2003), whereas CT60A/G (rs3087243) is important for efficient splicing and production of soluble CTLA-4, and may play a role in mRNA stability of sCTLA-4 (Ueda et al, 2003). A number of casecontrol studies have been conducted to investigate the association of these polymorphisms with SLE (Heward et al, 1999;Matsushita et al, 1999;Pullmann et al, 1999;D'Alfonso et al, 2000;Ahmed et al, 2001;Lee et al, 2001;Liu et al, 2001;Hudson et al, 2002;Aguilar et al, 2003;Barreto et al, 2004;Parks et al, 2004;Torres et al, 2004;Ulker et al, 2009;Chua et al, 2010;Kimkong et al, 2011). However, these studies have shown inconclusive or contradictory results.…”

Section: Introductionmentioning

confidence: 99%

CTLA-4 Polymorphisms and Systemic Lupus Erythematosus: A Comprehensive Meta-Analysis

Liu

2013

Genetic Testing and Molecular Biomarkers

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Objective: The aim of this study was to determine whether the SNPs + 49A/G and CT60A/G of the CTLA-4 gene are associated with susceptibility to systemic lupus erythematosus (SLE). Methods: The comprehensive metaanalysis for + 49A/G included 1753 cases and 2279 controls, and for CT60A/G included 676 cases and 576 controls. Allelic and genotypic comparisons between cases and controls were evaluated. For + 49A/G, we also subdivided it by population. Results: For + 49A/G, statistically significant differences were not noted

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Study of the CTLA-4 gene polymorphisms in systemic lupus erythematosus (SLE) samples from Malaysia

Cited by 34 publications

References 15 publications

Lack of association between Gly82Ser, 1704G/T and 2184A/G of RAGE gene polymorphisms and retinopathy susceptibility in Malaysian diabetic patients

Lack of association between Gly82Ser, 1704G/T and 2184A/G of RAGE gene polymorphisms and retinopathy susceptibility in Malaysian diabetic patients

Eosinophil cationic protein mRNA expression in children with bronchial asthma

CTLA-4 Polymorphisms and Systemic Lupus Erythematosus: A Comprehensive Meta-Analysis

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