Study of the distribution of Malassezia species in patients with pityriasis versicolor and healthy individuals in Tehran, Iran

Tarazooie, Bita; Kordbacheh, Parivash; Zaini, Farideh; Zomorodian, Kamiar; Saadat, Farshid; Zeraati, Hojjat; Hallaji, Zahra; Rezaie, Sassan

doi:10.1186/1471-5945-4-5

Cited by 93 publications

(130 citation statements)

References 33 publications

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“…The occurrence of Malassezia is verified in both sex (6,7,33) but the prevalence of pityriasis versicolor is observed more frequently in male (21,27), however other authors indicated that the incidence is higher in women (31). The role of sex in propensity to development of pityriasis versicolor is still unclear (37). Students age ranged from 10 to 17 years old, being 19 included 10-11; 35 of 12-13; 17 of 14-15 and one of 16-17 years old.…”

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confidence: 99%

Identification and pathogenicity of Malassezia species isolated from human healthy skin and with macules

Neves¹,

Magalhães²,

Silva³

et al. 2005

Braz. J. Microbiol.

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This study investigated the ocurrence of Malassezia species in clinically healthy students and with macules with a slight fawn discoloration and characterized the isolates as to the pathogenicity factors such as growth at 37ºC, lipase, phospholipase and protease detection. Clinical samples were collected from different body sites of one hundred students of different ages and both sexes. The samples, obtained by scrapping the skin surface and the scalp, were treated with potassa and cultured. Cultures were obtained in Petri dishes containing Sabouraud agar medium added of olive oil, incubated at room temperature and at 37ºC. Culture identifications were based in their morphological and physiological properties. Lipase, phospholipase and protease detection was performed in specific media on Petri dishes for formation of a zone. Globose, spherical yeast cells and hypha were investigated by direct microscopy of clinical materials. Malassezia furfur was detected in seven samples and M. sympodialis in four. All Malassezia cultures presented lipase activity, but none was phospholipase positive. Protease activity was observed in two M. furfur and two M. simpodialis isolates. Key words: Malassezia furfur, M. simpodialis, pathogenicityMalassezia is a lipophilic yeast frequently found in normal human skin. Current evidence indicates a high rate of skin colonization in healthy adults, in contrast with the low rate of colonization in prepubertal children (20).Gueho et al. (11) (14,17,35,38). With the exception of M. pachidermatis, the remaining eight species have an absolute in vitro requirement for suplementation of long-chain fatty acids. Based on studies conducted within the last 5 years, it appears that the most frequently organism associated with pityriasis versicolor may not be M. furfur and possible candidates include M. globosa (2,27) and M. sympodialis (15). The genus Malassezia was recently revised, but the clinical significance of each of these species is not clearly understood (15).Pityriasis versicolor (tinea versicolor) is a superficial fungal infection of the skin, appearing most commonly on the upper trunk, as well as on the upper arms, neck and face. As suggested by its name, pityriasis versicolor may be manifested in different colours, ranging from pink or to dark brown or even black. The lesions have a characteristic flaking appearance, although in larger lesions this can be evident only at the border of the macule, and can be hypopigmented or hyperpigmented. Generally, the lesions take the form of round or oval macules or papules, although in advanced cases the lesions may become confluent (13,21,33).

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confidence: 99%

Identification and pathogenicity of Malassezia species isolated from human healthy skin and with macules

Neves¹,

Magalhães²,

Silva³

et al. 2005

Braz. J. Microbiol.

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“…13,14 An Iranian study showed similar rates at 62%. 15 Higher rate of Malassezia colonization compared with the present study was also noticed in studies done on healthy human skin in Bosnia and Herzegovina (65%) and Sweden (84%). 16,17 Studies done in healthy Iranian, Indian and Tunisian healthy normal subjects yielded lower rate of colonization ranging from 31.2%-43%.…”

Section: Discussionmentioning

confidence: 46%

“…The highest detection rates of M. globosa is in agreement with studies from Iran Korea and Spain and stands in contrast with the other studies from India Canada and Russia in which they reported M. sympodialis as the commonest species, Bosnia and Herzegovina as M. restricta and Iran as M. furfur from normal healthy skin. 15,14,25,19,12,16,18 The differences between this study and others may not only be due to the result of variation in species on the skin of individuals in different countries, but also by the difference in sampling methods i.e. ; swabbing, a relatively insensitive method, scraping or adhesive transparent tape method, difference in culture media used (SDA with olive oil, mDA, LNA) and difference in incubation temperature of inoculated culture media.…”

Section: Discussionmentioning

confidence: 79%

“…A total of 120 apparently healthy men and 120 women without any skin diseases or who were not treated for Malassezia associated skin diseases were selected for the study. They were grouped according to their age as (0-10), (11)(12)(13)(14)(15)(16)(17)(18)(19)(20), (21)(22)(23)(24)(25)(26)(27)(28)(29)(30), (31-40), (41-50), (> 51) where each group consisted of 40 subjects (20 men and 20 women). The specimens consisted of scrapings collected from chest, back and neck from each subject totaling 720 sites.…”

Section: Methodsmentioning

confidence: 99%

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A study on malassezia micro flora in the skin of healthy individuals in North Kerala, India

Remya¹,

Arun²,

M³

et al. 2017

Int J Res Med Sci

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Background: Malassezia spp are accounted for approximately 60-90% of the total cutaneous fungal micro biota. An enhanced understanding of the distribution of Malassezia spp in healthy individuals is necessary to gain insight to involvement of these species in human skin disorders particularly in pityriasis versicolor. The aim of this study was to assess the Malassezia micro flora of healthy individuals and to identify species variation with different body sites, gender and age groups.Methods: A total of 120 apparently healthy men and 120 women without any skin diseases or who were not treated for Malassezia associated skin diseases were selected for the study. The specimens consisted of scrapings collected from chest, back and neck from each control subject totaling 720 sites.Results: The recovery rate of Malassezia was positive in case of 452 (62.78%) of 720 sites sampled, while the remaining 268 (37.22%) were found to be negative. Recovery rate of Malassezia was highest in chest with 83.33% positive cases, followed by the back with 73.33% and the neck 31.67%. M. globosa was identified as the common species (46.46%) followed by M. furfur (25%) and M. restricta (22.56%). No significant difference was present (P > 0.05) in distribution of species with different body sites, gender and age groups.Conclusions: The overall colonization rate of Malassezia spp in normal healthy individuals found in this study was 62.78%. M. globosa was identified as the common species (46.46%).

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“…19,40 The difference may be due to the fact that the centre of the PV lesions yields more viable material for culture 8,9,11 and avoid the isolation of surrounding commensal species. 38,44 In the patients studied, the most frequently isolated species in PV lesions were M. sympodialis (51%) and M. globosa (40%), followed by M. furfur (7%), M. obtusa (1%), and M. slooffiae (1%). These results are comparable with those founded by Canteros et al 5 in Buenos Aires city (central region of Argentina) and Giusiano et al 13 in Chaco city (Argentine northeast).…”

Section: Discussionmentioning

confidence: 99%

Prevalence of Malassezia species in patients with pityriasis versicolor in Rosario, Argentina

Ramadán

Sortino

Bulacio

et al. 2012

Revista Iberoamericana de Micología

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a b s t r a c tBackground: Malassezia species are considered opportunistic yeasts of increasing clinical importance. These lipophilic yeasts are associated with various human diseases, especially pityriasis versicolor (PV), a chronic superficial scaling dermatomycosis. Aims: The aim of this study was to isolate, identify and analyze the distribution of the different species of Malassezia in patients with PV in Rosario city (Argentina). Methods: A total of 264 clinical samples were studied. Isolates were identified on the basis of microscopic observation of cells, and physiological properties, such as the presence of catalase, ability to use Tween compounds, splitting of esculin, and morphology, color and precipitate production on chromogenic agar CHROMagar-Malassezia medium (CHROMM). Results: The highest prevalence of PV in this study was observed in the 25-to 45-year-old group. No differences were found in the development of PV between sexes. The most affected areas of body were the trunk and face. Malassezia sympodialis (51%) was the most commonly isolated species, followed in frequency by M. globosa (40%), Malassezia furfur (7%), Malassezia obtusa (1%) and Malassezia slooffiae (1%). Conclusions: The success for a correct identification of these yeasts is important to improve our knowledge about their epidemiological role in PV and also to detect the appearance of strains which are resistant to the commonly used antifungal drugs.

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Study of the distribution of Malassezia species in patients with pityriasis versicolor and healthy individuals in Tehran, Iran

Cited by 93 publications

References 33 publications

Identification and pathogenicity of Malassezia species isolated from human healthy skin and with macules

Identification and pathogenicity of Malassezia species isolated from human healthy skin and with macules

A study on malassezia micro flora in the skin of healthy individuals in North Kerala, India

Prevalence of Malassezia species in patients with pityriasis versicolor in Rosario, Argentina

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