Study of the Domestic Breeding Winter Rape Morphogenetic Potential Using in Vitro Culture

Chernobrovkina, Mariya; Khvatkov, Pavel; Leonteva, A.V.; Dolgov, Sergey

doi:10.17513/mjpfi.12010

Cited by 1 publication

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References 3 publications

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“…After drying in air flow laminar box, the explants were transferred onto filter paper, placed on a solid surface of MS medium supplemented with 1.0 mg•L −1 2,4-D in Petri dishes, and co-cultivated in light conditions at a temperature of 21 • C for 72 h. After co-cultivation period, the explants were washed with liquid MS medium, with a lack organic components but supplemented with 300 mg•L −1 Tm, and then transferred to the medium for explants cultivation and elimination of Agrobacterium (MS medium containing 1.0 mg•L −1 2,4-D and 150 mg•L −1 tmimentine (Tm) with the addition of 100 mg•L −1 kanamycin sulfate (Km)). Two weeks later, the explants were transferred to regeneration medium-MS medium supplemented with 4.0 mg•L −1 N 6 -benzyladenine, mg•L −1 Zeatin, 5.0 mg•L −1 AgNO 3 [47], and 150 mg•L −1 Tm and 100 mg•L −1 Km. Every 2 weeks, explants were transferred to fresh media.…”

Section: Tissue Culture Agrobacterium-mediated Transformation and Obtaining Generation Tmentioning

confidence: 99%

Genes with Cold Shock Domain from Eutrema salsugineum (Pall.) for Generating a Cold Stress Tolerance in Winter Rape (Brassica napus L.) Plants

et al. 2021

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The increased demand in vegetable oil for food purposes and high-protein feed for livestock and poultry encourages producers to expand the production of various oil crops, while occupying rather cold agroclimatic zones. Improved cold and frost resistance of cultivated crops would significantly increase the yield and expand the range of rape cultivation in a number of cold climate regions. Nine transgenic lines of winter rape containing genes encoding proteins with a cold shock domain (CspA и EsCSDP3) were obtained as a result of Agrobacterium transformation. In total, 260 explants were involved in transformation of rape using pBI121-CSPA-plant, with a transformation efficiency of 2.3%; among 750 explants using the pBI-EsCSDP3 construction, the efficiency was 0.4%. As a result of the studies, it was shown that the expression of the new gene Escsdp3 from the plant of Eutrema salsugineum was able to increase the cold and frost resistance of plants as effectively as the cspa gene from E. coli, which is classically used for this purpose. The cold resistance analysis of T1 transgenic plants generation revealed four cold resistant winter rape lines (three lines with the cspA-plant gene and one line with the Escsdp3 gene). The transfer of Escsdp3 and cspA-plant genes into winter rape plants led to a significant increase in frost resistance of plants. Two winter rapeseed lines were resistant to freezing (with the cspA-plant gene and with the Escsdp3 gene). Non-hardened transgenic plants remained viable after 24 h of exposure to negative temperatures up to −5 °C, and plants that passed through the hardening stage survived after freezing at −16 °C.

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Section: Tissue Culture Agrobacterium-mediated Transformation and Obtaining Generation Tmentioning

confidence: 99%

Genes with Cold Shock Domain from Eutrema salsugineum (Pall.) for Generating a Cold Stress Tolerance in Winter Rape (Brassica napus L.) Plants

et al. 2021

View full text Add to dashboard Cite

show abstract

Study of the Domestic Breeding Winter Rape Morphogenetic Potential Using in Vitro Culture

Cited by 1 publication

References 3 publications

Genes with Cold Shock Domain from Eutrema salsugineum (Pall.) for Generating a Cold Stress Tolerance in Winter Rape (Brassica napus L.) Plants

Genes with Cold Shock Domain from Eutrema salsugineum (Pall.) for Generating a Cold Stress Tolerance in Winter Rape (Brassica napus L.) Plants

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