Given the global importance of sweet potatoes as a nutrient-rich staple food, this research aimed to find the optimal cultivation practices to improve both yield and carotenoid content, with a particular focus on enhancing β-carotene content. In this study, the effects of different cultivation methods and plant densities on the agronomic parameters, physiological characteristics and carotenoid content of the ‘Beauregard’ variety were investigated across two consecutive growing seasons. Besides storage root yield, the key physiological parameters, including SPAD and chlorophyll fluorescence (Fv/Fm), were monitored to assess plant health and photosynthetic performance. Carotenoid content, including trans-β-carotene, cis-β-carotene, and ζ-carotene, was quantified using high-performance liquid chromatography (HPLC). Results indicated that the ridge cultivation method, particularly when combined with twin-row planting, consistently produced the highest yields, reaching the maximum of 40.87 t ha−1 in 2020. The flat cultivation method, especially in simple rows, showed the lowest yield. The analysis revealed that plant density had a more pronounced effect on yield and carotenoid content than the ridge or flat cultivation method alone. The maximum β-carotene content was achieved in the simple row (17,500 plants/ha) treatment planted on ridges with 247 µg/g. Significant correlations between both SPAD readings and Fv/Fm and yield were revealed, but no correlations with storage root carotenoid content were found. This suggests that, while these leaf physiological traits can be used to estimate the yield, they are not directly associated with the carotene content of the storage root. The study highlights the ridge cultivation and 35,000 plants/ha method as a stable and high-yielding option for ‘Beauregard’ in terms of improving and balancing the yield and carotenoid content; however, reducing the plant density resulted in elevated carotenoid content with significant yield reductions. The findings contribute to the understanding of how agronomic practices influence the nutritional and physiological traits of sweet potatoes, with implications for improving food security and nutritional outcomes in sweet potato cultivation.