Study of the estrogenic-like mechanism of glycosides of cistanche using metabolomics

Song, Hui; Li, W. L.; Liu, B. M.; Sun, Xiang-Ming; Ding, Jing‐Xin; Chen, N.; Ji, Yu; Zou, Xiang

doi:10.1039/c7ra06930h

Cited by 7 publications

(3 citation statements)

References 33 publications

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“…Abnormal changes, such as fumarate, malate and α-ketoglutaric acid, belonging to the citric acid cycle, confirm this view. The citric acid cycle, as the linkage among sugar, lipids and amino acid metabolism, is the most important energy source for the body [25]. In addition, other amino acids are substrates for metabolic energy, which could be used for the synthesis of specific proteins, peptides and other nitrogenous compounds [26].…”

Section: Resultsmentioning

confidence: 99%

Metabolomics analysis of baicalin on ovalbumin-sensitized allergic rhinitis rats

Chen

Shu

et al. 2019

R. Soc. open sci.

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Allergic rhinitis (AR) is a global health problem that appears in all age groups and affects approximately 15–30% of people. Baicalin has been used for the treatment of various allergic diseases, including AR. However, the metabolic mechanisms of AR and baicalin against AR have not been systematically studied. Here, ovalbumin-sensitized AR rats were used as a model, and animal behaviour, histological analysis, enzyme-linked immunosorbent assay (ELISA) and metabolomics were used to elucidate the mechanism of baicalin for AR. The results indicated that baicalin has a protective effect on AR rats by inhibiting the release of immunoglobulin E (IgE), histamine, interleukin-1 beta (IL-1β), interleukin-4 (IL-4), interleukin-6 (IL-6) and tumour necrosis factor alpha (TNF-α). In addition, ovalbumin-induced AR included modulation of arachidonic acid, leukotriene A4 (LTA4), leukotriene B4 (LTB4), α-ketoglutaric acid, phosphatidylcholine PC (20 : 4/0 : 0), PC (16 : 0/0 : 0), citric acid, fumarate, malate, 3-methylhistidine, histamine and other amino acids that are involved in arachidonic acid, histidine metabolism, the TCA cycle and amino acid metabolism. Thus, AR could be alleviated or reversed by baicalin.

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Section: Resultsmentioning

confidence: 99%

Metabolomics analysis of baicalin on ovalbumin-sensitized allergic rhinitis rats

Chen

Shu

et al. 2019

R. Soc. open sci.

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“…To elucidate the mechanism of F-G in the treatment of liver injury, three groups of serum samples, including that from the normal control group, model control group, and F-G group (30 g/kg), were used for metabolic analysis by UPLC-MS according to a method published by the authors with minor modifications[ 35 ].…”

Section: Methodsmentioning

confidence: 99%

Metabolomics of Fuzi-Gancao in CCl₄induced acute liver injury and its regulatory effect on bile acid profile in rats

Wang¹,

Zhao²,

Thapa³

et al. 2021

WJG

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BACKGROUND Fuzi ( Radix aconiti lateralis)- Gancao ( Radix glycyrrhizae ) is one of the most classical drug pairs of traditional Chinese medicine. In clinical practice, decoctions containing Fuzi-Gancao (F-G) are often used in the treatment of liver diseases such as hepatitis and liver failure. AIM To investigate the metabolomics of F-G in CCl 4 induced acute liver injury in rats and its regulatory effect on the bile acid profile. METHODS The pharmacodynamic effect of F-G on CCl 4 induced acute liver injury in rats was evaluated, and an ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) method for the simultaneous determination of 92 metabolites from multiple pathways was established to explore the protective metabolic mechanism of F-G in serum on the liver. RESULTS Twenty-four differential metabolites were identified in serum samples. The primary bile acid biosynthetic metabolic pathway was the major common pathway in the model group and F-G group. Subsequently, a UPLC-MS/MS method for simultaneous determination of 11 bile acids, including cholic acid, ursodeoxycholic acid, glycochenodeoxycholic acid, glycochenodeoxycholic acid, taurocholic acid, glycocholic acid, chenodeoxycholic acid, deoxycholic acid, taurochenodeoxycholic acid, taurocholic acid, and glycinic acid, was established to analyze the regulatory mechanism of F-G in serum. F-G decreased the contents of these 11 bile acids in serum in a dose-dependent manner compared with those in the model control group. CONCLUSION F-G could protect hepatocytes by promoting the binding of free bile acids to glycine and taurine, and reducing the accumulation of free bile acids in the liver. F-G could also regulate the compensatory degree of taurine, decreasing the content of taurine-conjugated bile acids to protect hepatocytes.

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“…Its stem, also known as Rou Cong Rong (Wang et al, 2012), is a precious traditional Chinese medicine and is usually used as a tonic (Ai et al, 2021;. It can nourish the kidneys, relax the bowels, and protect the liver, exerting anti-oxidant, anti-aging, anti-fatigue, immunoregulation, and estrogen-like effects (Li et al, 2020;Piwowarczyk et al, 2020;Song et al, 2017). It mainly consists of phenylethanol glycosides, iridoids, lignanoids, alkaloids, and polysaccharides (Fu et al, 2020;Tu et al, 2007).…”

Section: Introductionmentioning

confidence: 99%

Identification of Absorbed Constituents and their Metabolites Related to Estrogen-Like Activity of Total Glycosides of Cistanche deserticola in Rat Serum

Yang¹,

Ding²,

Xu³

et al. 2023

PJZ

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Total glycosides of C. deserticola, Estrogen-like activity, UPLC-Q-TOF-MS, in vivo active compounds, Absorbed constituentsCistanche deserticola Y. C. Ma is a kind of traditional Chinese medicine and food raw material with estrogen-like effect; its active ingredients are glycosides. To explore the absorbed constituents of total glycosides (TGs) in vivo and its estrogen-like activity, and reveal its direct acting substances in the body, in this study, 30 female Wistar rats were orally administered with TGs, and serum fingerprints of blood samples collected at 10 different times were established by UPLC-Q-TOF-MS analysis. The estrogen-like activity of TGs was evaluated by determining the proliferation rate of Michigan Cancer Foundation-7 cells. The spectrum-effect relationship was analyzed by serum fingerprint of TGs to characterize the activity in vivo and identify the active compounds of TGs. In the results, a total of 75 chemical compounds of TGs were identified in serum, including 11 parent molecules and 64 metabolites. The serum fingerprint of TGs were established, and 36 common peaks were identified. The correlation coefficient and the Grey relational degree between the relative area of the common peaks and the estrogen-like activity of TGs were determined through bivariate analysis and Grey relational analysis, respectively. Total 32 chemical components were identified, including methylated cistantubuloside B, acetylated tubuloside B, and acteoside, which are the active compounds leading to the estrogen-like activity of TGs in vivo. In conclusion, this study is expected to provide a theoretical reference for subsequent in-depth studies on the estrogen-like pharmacodynamic material basis of TGs.

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Study of the estrogenic-like mechanism of glycosides of cistanche using metabolomics

Abstract: Cistanche deserticola, known as Rou Cong-Rong in China, has been used as a tonic for more than 1800 years, with previous studies demonstrating that glycosides of cistanche (GCs) are a main active component.

Cited by 7 publications

References 33 publications

Metabolomics analysis of baicalin on ovalbumin-sensitized allergic rhinitis rats

Metabolomics analysis of baicalin on ovalbumin-sensitized allergic rhinitis rats

Metabolomics of Fuzi-Gancao in CCl₄induced acute liver injury and its regulatory effect on bile acid profile in rats

Identification of Absorbed Constituents and their Metabolites Related to Estrogen-Like Activity of Total Glycosides of Cistanche deserticola in Rat Serum

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Study of the estrogenic-like mechanism of glycosides of cistanche using metabolomics

Abstract: Cistanche deserticola, known as Rou Cong-Rong in China, has been used as a tonic for more than 1800 years, with previous studies demonstrating that glycosides of cistanche (GCs) are a main active component.

Cited by 7 publications

References 33 publications

Metabolomics analysis of baicalin on ovalbumin-sensitized allergic rhinitis rats

Metabolomics analysis of baicalin on ovalbumin-sensitized allergic rhinitis rats

Metabolomics of Fuzi-Gancao in CCl4 induced acute liver injury and its regulatory effect on bile acid profile in rats

Identification of Absorbed Constituents and their Metabolites Related to Estrogen-Like Activity of Total Glycosides of Cistanche deserticola in Rat Serum

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Metabolomics of Fuzi-Gancao in CCl₄induced acute liver injury and its regulatory effect on bile acid profile in rats