Study of the genetic variability of porcine circovirus type 2 detected in Serbia and Slovenia

Toplak, Ivan; Lazić, Sava; Lupulović, Diana; Radulović, Jasna Prodanov; Becskei, Zsolt; Došen, Radoslav

doi:10.1556/avet.2012.035

Cited by 13 publications

(18 citation statements)

References 16 publications

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“…These latter analyses were carried out using the complete PCV2 genome, the complete ORF1, a partial ORF1 sequence, the complete ORF2 and a partial ORF2 sequence. Partial ORF1 and ORF2 analyses were performed since only partial ORF1 and ORF2 sequences were recovered from previous Croatian and neighbour countries (Slovenia and Serbia) sequences (Toplak and others 2012). …”

Section: Methodsmentioning

confidence: 99%

Origin of porcine circovirus type 2 (PCV2) from swine affected by PCV2‐associated diseases in Croatia

et al. 2014

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Porcine circovirus type 2 (PCV2) causes some of the most significant economic losses in pig production. Several multisystemic syndromes have been attributed to PCV2 infection, which are known as PCV2-associated diseases (PCVDs). This study investigated the origin and evolution of PCV2 sequences in domestic pigs and wild boars affected by PCVDs in Croatia. Viral sequences were recovered from three wild boars diagnosed with PCV2-systemic disease (PCV2-SD), 63 fetuses positive for PCV2 DNA as determined by PCR, 14 domestic pigs affected with PCV2-SD (displaying severe interstitial nephritis) and five domestic pigs with proliferative and necrotising pneumonia. Seventeen complete PCV2 genomes were recovered. Phylogenetic and evolutionary analyses based on median-joining phylogenetic networks, amino acid alignments and principal coordinate analysis were performed using complete genomes, as well as complete and partial ORF sequences for ORF1 and ORF2. Two of the 17 PCV2 sequences belonged to PCV2a, 14 to PCV2b and one was unclustered. PCV2b was the predominant genotype in Croatia and has been linked to international trade as a route of introduction. Correlation between particular viral strains with PCVDs is lacking.

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Section: Methodsmentioning

confidence: 99%

Origin of porcine circovirus type 2 (PCV2) from swine affected by PCV2‐associated diseases in Croatia

et al. 2014

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“…In the neighbouring country Serbia besides immunohistochemistry, PCR was successfully used for the detection and later on for molecular characterization of circulating PCV2 strains [8,9]. Ogawa et al [13] examined 33 principal organs collected from PRDC/PMWS-suspected pigs and 26 organs of aborted foetuses by multiplex PCR for the presence of PCV2 and PPV.…”

Section: Discussionmentioning

confidence: 99%

“…The standard laboratory methods for diagnosis of diseases caused by PCV2 are mainly based on immunohistochemistry and in situ hybridization directly in tissue samples or by real-time quantitative polymerase chain reaction [5,6]. Also the conventional polymerase chain reaction is often used as a standard method for detection of PCV2 in clinical samples [7][8][9].…”

Section: Introductionmentioning

confidence: 99%

“…The genome contains two major ORFs (ORF1 and ORF2) which encode non-structural (NS1, NS2 and NS3) and structural proteins (VP1, VP2 and VP3). Although the VP1/VP2 genes are highly conserved, PPV strains can be distinguished by their different pathogenicity [8]. Virus isolation in cell cultures is laborious and time consuming, and it cannot be achieved for all PPV strains.…”

Section: Introductionmentioning

confidence: 99%

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Molecular Detection of PCV2 And PPV in Pigs in Republic of Srpska, Bosnia and Herzegovina

et al. 2016

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The presence of porcine circovirus 2 and porcine parvovirus was examined in forty clinical samples of spleen, lymph nodes and lungs originating from non-vaccinated swine by polymerase chain reaction. All animals were reared in extensive livestock farming systems in different geographical districts of Republic of Srpska, Bosnia and Herzegovina. Porcine circovirus 2 DNA was detected in four lymph node and two spleen samples (15%), while porcine parvovirus DNA was identifi ed in fi ve lymph node samples (12.5%). The presence of both viruses was detected in three lymph node samples (7.5%). Partial nucleotide sequence of ORF1 gene of 2 porcine circovirus 2 and VP2 gene of 2 porcine parvovirus isolates was determined. The nucleotide sequences of two PCV2 isolates from RS-BIH included in phylogenetic typing are similar and cluster together with the strain Mantova isolated from domestic pigs in Italy, strains DE006-14 and DE222-13 isolated from pigs in Germany as well as with the strain Jvnan isolated from pigs in China. Also, analyzed PCV2 isolates were partially similar to the strain NIV-C SRB isolated from pigs in Serbia. The nucleotide sequences of two PPV isolates that were included in phylogenetic typing showed a high level of similarity with the strain Challenge isolated from pigs in UK, strain Kresse isolated from pigs in USA and strains 77 and LZ isolated from pigs in China.

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“…In Serbia, the PCV2 related attention has been focused on the problem of growing pigs, but not on sows in service, even though PCV2 infection can be transmitted by semen (Schmoll et al, 2008). Study of 30 Serbian PCV2 sequences indicated that the predominant genotype in Serbia is PCV2b which is closely related to those previously described in Europe (Toplak et al, 2012;Savić et al, 2012). Vaccination against PCV2 is not obligatory and is being implemented mainly after the occurrence of clear clinical features of the disease.…”

Section: Introductionmentioning

confidence: 91%

Investigation of Porcine Circovirus type 2 (PCV2) antibodies in clinically healthy boars from Serbian commercial farms

Bojkovski

Ilić

Vasić

et al. 2018

J Hellenic Vet Med Soc

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ABSTRACT. The aim of the present study was to determine the porcine circovirus type 2 (PCV2) prevalence in boars in 3 farms in Serbia and their possible relation with alterations of reproductive parameters and blood biochemical parameters [total protein, urea, creatinine and aspartate transaminase levels (AST)]. The prevalence of (PCV2) was evaluated by the presence of specific antibodies. An ELISA assay was used for the detection of PCV2 antibodies in 58 boars' blood sera from Serbian commercial farms, among which 48 were from and reared in the Serbian farms, and 10 were imported from different European Union (EU) countries.Anti PCV2 IgM and/or IgG were detected in sera of 51 (87.93%) boars. Based on the type of antibodies (IgM and IgG PCV2 antibodies), it was concluded that chronic PCV2 infection was the predominant type in tested boars. The imported boars did not have an active infection titer. Biochemical tests in blood didn't show significant differences between PCV2 positive and negative boars. The recorded high prevalence of PCV2 antibodies among boars indirectly proved that PCV2 has been wide spread in the examined farms. Significant difference in the frequency of active, recent and chronic PCV2 infection in boars was found among three farms (p<0.05). Imported boars did not show significant difference in terms of active, recent or chronic PCV2 infection in comparison with boars deriving from Serbian farms http://epublishing.ekt.gr | e-Publisher: EKT | Downloaded at 11/05/2018 18:48:27 |

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Study of the genetic variability of porcine circovirus type 2 detected in Serbia and Slovenia

Cited by 13 publications

References 16 publications

Origin of porcine circovirus type 2 (PCV2) from swine affected by PCV2‐associated diseases in Croatia

Origin of porcine circovirus type 2 (PCV2) from swine affected by PCV2‐associated diseases in Croatia

Molecular Detection of PCV2 And PPV in Pigs in Republic of Srpska, Bosnia and Herzegovina

Investigation of Porcine Circovirus type 2 (PCV2) antibodies in clinically healthy boars from Serbian commercial farms

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