Study of the gut microbiome in Egyptian patients with active ulcerative colitis

Ahmed, E.A.; Ahmed, Saad; Zakaria, N.H.; Baddour, N.M.; Header, Doaa

doi:10.1016/j.rgmxen.2022.07.006

Cited by 6 publications

(6 citation statements)

References 24 publications

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“…There is a connection between gut microbiota and the UC with phenomenal advances in sequencing techniques. Several studies have supported the hypothesis that the gut ecosystem is crucial in UC onset and development ( Castro-Dopico and Clatworthy, 2019 ; Zeng et al, 2021 ; Ahmed et al, 2022 ).…”

Section: Introductionmentioning

confidence: 91%

In vivo evidence of the prevents DSS-induced colitis of Lactiplantibacillus plantarum L15

et al. 2022

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Ulcerative colitis (UC) is challenging to treat and severely impacts patients and families. A previous study reported immunomodulatory and reduction of pro-inflammatory properties for the Lactiplantibacillus plantarum L15. This study aimed to analyze the preventive properties and mechanistic actions in an in vivo colitis model. The histopathological alteration, inflammation cytokines, and intestinal barrier function were analyzed. Subsequently, the cecal gut microbiota contents and products from different groups were detected. Finally, gene expressions related to the NF-κB signaling process were evaluated. L. plantarum L15 significantly decreased disease activity index (DAI), myeloperoxidase activity (MPO), pro-inflammatory cytokine (TNF-α, IL-1β, and IL-6) level, and increased weight change, colon length, and production of inflammation-suppressing cytokines. Furthermore, this strain supplementation substantially increased ZO-1, Occludin, and Claudin-1, and MUC2 mRNA expression levels with a corresponding decrease in serum lipopolysaccharide and D-lactic acid contents. In addition, L. plantarum L15 improved gut microbiota composition and increased short-chain fatty acid (SCFAs) in the colon content, which significantly reduced the transfer of NF-κB p65 to the nucleus. Our findings provide a theoretical basis for L. plantarum L15 as a preventive candidate for UC.

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Section: Introductionmentioning

confidence: 91%

In vivo evidence of the prevents DSS-induced colitis of Lactiplantibacillus plantarum L15

et al. 2022

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“… 53 It has also been reported that there was no significant difference in the abundance of Bifidobacterium between UC patients and healthy people. 54 Meanwhile, our research found that selenium yeast increased the abundance of Prevotella . It has been found that Prevotella is abundant in UC patients with continuous remission.…”

Section: Discussionmentioning

confidence: 78%

“… 53 However, another study found that there is no significant difference in the abundance of Prevotella between UC patients and healthy people. 54 These opposite findings indicate that the gut microbiota might play a complex and dynamic role in the occurrence and development of IBD, which needs further research.…”

Section: Discussionmentioning

confidence: 99%

Selenium Yeast Alleviates Dextran Sulfate Sodium-Induced Chronic Colitis in Mice by Reducing Proinflammatory Cytokines and Regulating the Gut Microbiota and Their Metabolites

Wu,

Li,

Jiang

et al. 2024

JIR

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Background Inflammatory bowel disease (IBD) is a chronic recurrent gastrointestinal inflammatory disease. Selenium has been reported to have therapeutic potential in IBD. Selenium yeast is a common selenium supplement that is convenient to access. This study explored the effect of selenium yeast on dextran sulfate sodium- (DSS-)induced chronic colitis in mice. Methods Mice were randomly divided into four groups: the control group, selenium yeast group, chronic colitis group, and chronic colitis+selenium yeast group (n=6). Mice were killed on the 26th day. The disease activity index (DAI) score and histological damage score were calculated. Cytokines, serum selenium, colonic tissue selenium, gut microbiota and their metabolites short-chain fatty acids (SCFAs) were evaluated. Results Selenium yeast lowered IL-1β, IL-6, TNF-α, IL-17A, IL-22 and IFN-γ ( P <0.05). In addition, selenium yeast significantly elevated Turicibacter, Bifidobacterium, Allobaculum, Prevotella, Halomonas, Adlercreutzia ( P <0.05), and butyric acid ( P <0.05). Conclusion Selenium yeast could improve DSS-induced chronic colitis in mice by regulating cytokines, gut microbiota and their metabolites.

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“…Ahmed et al . performed a study on 21 active UC patients, F. prausnitzii was higher in mild than in moderate and severe cases; however, the difference was not statistically significant [ 18 ]. On the other hand, Liu et al .…”

Section: Discussionmentioning

confidence: 99%

Study of Fecalibacteria prausntzii in Egyptian patients with inflammatory bowel disease

Abd El Atty,

Nouh,

Abdelsalam

et al. 2024

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Introduction Inflammatory bowel diseases (IBD) are characterised by chronic inflammation of the gastrointestinal tract. There aetiopathogenesis has not yet been fully elucidated. Faecalibacterium prausnitzii is one of the most abundant in human gut, and it is found in about 5% of the gut microbiota. Aim To study the role of F. prausnitzii in Egyptian patients with ulcerative colitis (UC) and Crohn’s disease (CD) and to assess its relationship with the disease activity. Material and methods This study includes 80 patients divided as follows: group I: 30 patients with UC; group II: 30 patients with CD; and group III: 20 healthy patients as control. DNA execration was performed using a faecal extraction kit and quantitative SYBR green real time PCR to identify the core of F. prausnitzii . Results In group 1 of UC patients, the level of bacteria was reduced by a mean of 1.68E-01, and in the control group by a mean of 2.72E-01 ( p 2 = 0.004, significant). Regarding F. prausnitzii in UC patients according to Mayo score, in severe patients the level of bacteria was reduced significantly by a median of 4.80E-02. Regarding F. prausnitzii in group 2 of CD, the level of bacteria was significantly reduced by mean of 1.70E-01, and in the control group by a mean of 2.72E-01 ( p 3 = 0.037, significant). Conclusions There was a significant difference between CD and UC patients and the control group in F. prausnitzii . There was a significant reduction in the level of F. prausnitzii in severe UC cases.

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Study of the gut microbiome in Egyptian patients with active ulcerative colitis

Cited by 6 publications

References 24 publications

In vivo evidence of the prevents DSS-induced colitis of Lactiplantibacillus plantarum L15

In vivo evidence of the prevents DSS-induced colitis of Lactiplantibacillus plantarum L15

Selenium Yeast Alleviates Dextran Sulfate Sodium-Induced Chronic Colitis in Mice by Reducing Proinflammatory Cytokines and Regulating the Gut Microbiota and Their Metabolites

Study of Fecalibacteria prausntzii in Egyptian patients with inflammatory bowel disease

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