Study of the immunogenicity of hepatitis B surface antigen synthesized in transgenic potato plants with increased biosafety

Рукавцова, Е. Б.; Rudenko, N. V.; Puchko, Elena N.; Захарченко, Н. С.; Buryanov, Yа. I.

doi:10.1016/j.jbiotec.2015.03.019

Cited by 18 publications

(8 citation statements)

References 23 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Few recent reports also show the expression of antigens against hepatitis B. In one of these reports, oral immunogenicity of the HBsAg synthesized in the tubers of marker-free potato plants was demonstrated (Rukavtsova et al 2015 ). In this study, mice fed with transgenic potato tubers, showed an increase in the level of HBsAg antibodies significantly above the protective value, which was maintained for 1 year after the immunization.…”

Section: Hepatitismentioning

confidence: 99%

Need of cost-effective vaccines in developing countries: What plant biotechnology can offer?

et al. 2016

View full text Add to dashboard Cite

To treat current infectious diseases, different therapies are used that include drugs or vaccines or both. Currently, the world is facing an increasing problem of drug resistance from many pathogenic microorganisms. In majority of cases, when vaccines are used, formulations consist of live attenuated microorganisms. This poses an additional risk of infection in immunocompromised patients and people suffering from malnutrition in developing countries. Therefore, there is need to improve drug therapy as well as to develop next generation vaccines, in particular against infectious diseases with highest mortality rates. For patients in developing countries, costs related to treatments are one of the major hurdles to reduce the disease burden. In many cases, use of prophylactic vaccines can help to control the incidence of infectious diseases. In the present review, we describe some infectious diseases with high impact on health of people in low and middle income countries. We discuss the prospects of plants as alternative platform for the development of next-generation subunit vaccines that can be a cost-effective source for mass immunization of people in developing countries.

show abstract

Section: Hepatitismentioning

confidence: 99%

Need of cost-effective vaccines in developing countries: What plant biotechnology can offer?

et al. 2016

View full text Add to dashboard Cite

show abstract

“…70 mIU/mL. In the absence of adjuvant, primary immunization with 40 µg oral doses of potato-expressed antigen did not induce a response ( 6 ), but otherwise with 60 µg oral doses antigen prompted an antibody response up to 170 mIU/mL ( 26 ). In this study, a positive response was obtained by feeding transgenic F. velutipes fruiting bodies without CT, indicating that the mushroom polysaccharides might serve the role of an adjuvant.…”

Section: Resultsmentioning

confidence: 99%

Development of a Transgenic Flammulina velutipes Oral Vaccine for Hepatitis B

Huang¹,

Lin

Lyu³

et al. 2019

Food technol. biotechnol. (Online)

View full text Add to dashboard Cite

Orally administered fungal vaccines show promise for the prevention of infectious diseases. Edible mushrooms are deemed appropriate hosts to produce oral vaccines due to their low production cost and low risk of gene contamination. However, their low expression level of antigens has limited the potential development of oral vaccines using mushrooms. The low expression level might result from impurity of the transgenic mycelia since dikaryotic mycelia are commonly used as transformation materials. In this study, stable transgenic hepatitis B virus surface antigen (HBsAg) in Flammulina velutipes transformants was obtained by Agrobacterium-mediated transformation, followed by fruiting and basidiospore mating. The formation of HBsAg was detected by western blot analysis. The expression levels of HBsAg in transgenic F. velutipes fruiting bodies were (129.3±15.1), (110.9±1.7) and (161.1±8.5) ng/g total soluble protein. However, the values may be underestimated due to incomplete protein extraction. Two of the four pigs in the experimental group produced positive anti-HBsAg-specific IgG after being fed the HBsAg transgenic F. velutipes fruiting bodies for 20 weeks, while no anti-HBsAg antibody was detected in the control group. One of the positive pigs had HBsAg titres of 5.36 and 14.9 mIU/mL in weeks 10 and 14, respectively, but expression faded thereafter. The other positive pig displayed HBsAg titres of 9.75, 17.86 and 39.87 mIU/mL in weeks 14, 18 and 20, respectively. The successful immunogenicity in pigs fed transgenic F. velutipes fruiting bodies demonstrated the potential of using the fungus as an oral vaccine.

show abstract

“…Plant based vaccines via food stuff have been investigated for the delivery of viral antigens for vaccinations with the delivery of Hepatitis B antigens on transgenic potatoes being the first proof of concept in the delivery of immune response via food. 76 Plant-based lectins have been investigated as potential agents to inhibit HIV in its initial establishment of infection and other viral disease. 60,68 Additional studies have shown the production of antiviral lechtins and proteins as vaccines in tobacco and lettuce crops as a cost effective measure for the mass production of plant based vaccines that is extendable to the wider developing world.…”

Section: Plant Based Vaccinesmentioning

confidence: 99%

The 2020 Coronavirus (COVID-19) Global Pandemic, a Call to Arms to Pharmacognosy Researchers – Plant-Based Antiviral Approaches

Alcorn¹

2021

View full text Add to dashboard Cite

Background:The unprecedented modern pandemic that is COVID-19 has awakened a global fear of viral disease and an activation of viral targeted drug discovery not seen before in modern times. Whilst the vaccines about to enter the market, are an important component of the strategy to combat COVID-19, vaccination should be considered a part of a wider strategy to address this and future zoonotic viral outbreaks. Plant-based approaches may provide valuable avenues for viral disease management. Methods: This commentary combines an appraisal of the existing literature with the authors thoughts on key challenges that pharmacognosy may be able to assist with, in the management of zoonotic viral disease. Results: There are many avenues for plant-based antiviral research in the context of the present COVID-19 pandemic. This narrative review discusses the use of plant-based approaches; as viral drug leads, as agents for managing the cytokine storm associated with acute respiratory distress syndrome, as agents for elimination of viral reservoirs and as agents in hand sanitisation. Conclusion: Whilst the arrival of vaccines is an important step in the response to the current COVID-19 pandemic. Pharmacognosy still has a role to play in the management of this and future viral pandemics. The support of researchers in this domain is needed to combat viral pandemics via arming clinicians with a broader range of options for prevention and treatment of viral illness on a global scale.

show abstract

Study of the immunogenicity of hepatitis B surface antigen synthesized in transgenic potato plants with increased biosafety

Cited by 18 publications

References 23 publications

Need of cost-effective vaccines in developing countries: What plant biotechnology can offer?

Need of cost-effective vaccines in developing countries: What plant biotechnology can offer?

Development of a Transgenic Flammulina velutipes Oral Vaccine for Hepatitis B

The 2020 Coronavirus (COVID-19) Global Pandemic, a Call to Arms to Pharmacognosy Researchers – Plant-Based Antiviral Approaches

Contact Info

Product

Resources

About