Study of the influence of over-the-counter vitamin supplement intake on urine fluorescence to optimize cancer detection by fluorescence cystoscopy

Zellweger, Matthieu; Martoccia, Carla; Mengin, Matthieu; Iselin, Christophe; Bergh, Hubert van den; Wagnières, Georges

doi:10.1117/1.jbo.20.6.066011

Cited by 4 publications

(8 citation statements)

References 20 publications

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“…In contrast, a peak at 470 ± 10 nm with varying intensities is clearly seen for all the urine samples. It is well established that the inherent fluorescence and color of the urine samples vary according to the dietary intake, body metabolism, pre-existing complications, and age. , Therefore, fluorometric and colorimetric assays are influenced by the inherent fluorescence of urine that may interfere with the assay responses, especially for the detection of less abundant biomarkers.…”

Section: Results and Discussionmentioning

confidence: 99%

Colorimetric Urinalysis for On-Site Detection of Metabolic Biomarkers

Yeasmin

Ammanath

Ali

et al. 2020

ACS Appl. Mater. Interfaces

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Over the past few decades, colorimetric assays have been developed for cost-effective and rapid on-site urinalysis. Most of these assays were employed for detection of biomarkers such as glucose, uric acid, ions, and albumin that are abundant in urine at micromolar to millimolar levels. In contrast, direct assaying of urinary biomarkers such as glycated proteins, low-molecular-weight reactive oxygen species, and nucleic acids that are present at significantly lower levels (nanomolar to picomolar) remain challenging due to the interferences from the urine sample matrix. State-of-the-art assays for detection of trace amounts of urinary biomarkers typically utilize time-consuming and equipment-dependent sample pretreatment or clean-up protocols prior to assaying, which limits their applicability for on-site analysis. Herein, we report a colorimetric assay for on-site detection of trace amount of generic biomarkers in urine without involving tedious sample pretreatment protocols. The detection strategy is based on monitoring the changes in optical properties of poly(3-(4-methyl-3′-thienyloxy)propyltriethylammonium bromide) upon interacting with an aptamer or a peptide nucleic acid in the presence and absence of target biomarkers of relevance for the diagnosis of metabolic complications and diabetes. As a proof of concept, this study demonstrates facile assaying of advanced glycation end products, 8-hydroxy-2′-deoxyguanosine and hepatitis B virus DNA in urine samples at clinically relevant concentrations, with limits of detection of ∼850 pM, ∼650 pM, and ∼ 1 nM, respectively. These analytes represent three distinct classes of biomarkers: (i) glycated proteins, (ii) low-molecular-weight reactive oxygen species, and (iii) nucleic acids. Hence, the proposed methodology is applicable for rapid detection of generic biomarkers in urine, without involving sophisticated equipment and skilled personnel, thereby enabling on-site urinalysis. At the end of the contribution, we discuss the opportunity to translate the homogeneous assay into a paper-based format.

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Section: Results and Discussionmentioning

confidence: 99%

Colorimetric Urinalysis for On-Site Detection of Metabolic Biomarkers

Yeasmin

Ammanath

Ali

et al. 2020

ACS Appl. Mater. Interfaces

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“…14 It has been shown that the metabolization of consumed over-the-counter (OTC) vitamin supplements showed the highest fluorescence values of the urine at 8−10 h after intake of the OTC. 15 Baseline levels were reached 24 h after intake. 15 According to that study, the OTC vitamin supplement generates fluorescence in urine, which might lead to a wrong interpretation of diagnostic results using fluorescence cystoscopy.…”

Section: Introductionmentioning

confidence: 99%

“…15 Baseline levels were reached 24 h after intake. 15 According to that study, the OTC vitamin supplement generates fluorescence in urine, which might lead to a wrong interpretation of diagnostic results using fluorescence cystoscopy. Therefore, it was recommended to avoid vitamin supplements during the week performing a fluorescence cystoscopy.…”

Section: Introductionmentioning

confidence: 99%

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Influence of vitamins and food on the fluorescence spectrum of human urine

Chalissery,

Homann,

Stepp

et al. 2024

Lasers Surg Med

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ObjectivesFluorescence spectroscopy of human urine is a method with the potential to gain importance as a diagnostic tool in the medical field, e.g., for measuring Coproporphyrin III (CPIII) as an indicator of cancer and acute types of porphyria. Food can change human urine's color, which could influence the urine fluorescence spectrum and the detection of CPIII in urine. To determine if there is a noticeable influence on the urine fluorescence spectrum or on the detection of CPIII in urine, 16 vitamin supplements, and three food items were tested. Such investigation may also prevent false interpretation of measured data.MethodsUrine samples were collected before and after (overnight, ca. 8 h) intake of each test substance. Samples were investigated by fluorescence spectrum analysis. At excitation wavelengths from 300 to 500 nm and emission wavelengths from 400 to 700 nm excitation‐emission‐matrices were measured. Data obtained from urine before intake were compared to the data from overnight urine. Furthermore, the investigation of any interference with the CPIII concentration was performed at an excitation wavelength of 407 ± 3 nm and emission wavelengths of 490−800 nm.ResultsOnly vitamin B2, but none of the other tested substances, showed noticeable influence on the urine fluorescence spectrum. None of the tested substances showed noticeable interference with the recovery rate of CPIII.ConclusionsThe correct interpretation of measured data by fluorescence spectroscopy is possible with the exception if vitamin B2 supplementation was performed; thus, the consumption of vitamin B2 supplements before fluorescence testing of the patient's urine should be avoided and/or must be requested. CPIII concentrations could reliably be measured in all cases.

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“…Known compounds with altered fluorescence intensity observed in case of some cancers (bladder, breast, etc.) include free (unbound) NAD(P)H molecules, flavins, porphyrins, proteins and pigments [1][2][3][4].…”

Section: Introductionmentioning

confidence: 99%

Fluorescence biomarkers of malignant melanoma detectable in urine

et al. 2020

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Malignant melanoma (MM) is a cancerous transformation of melanocytes. It is a disease with the worst response to therapy and, compared to other malignancies, presents much earlier with metastases. MM still belongs to relatively late-detected malignant diseases. Even so, the MM mortality rate is up to 96% for a relatively small incidence (5%). The gold standard for MM diagnosis is a histopathological examination that requires invasive surgery. An invasive sampling method of a biological material can be a stressful factor for the patient, which is often the reason why patients do not seek medical assistance as soon as possible. Our goal was to find a link between metabolites in urine and the stage of MM. Two excitation peaks at 360–370 nm and 450 nm were characterised in spectra of urine samples. The emission spectra have shown one significant peak at 410–460 nm. After addition of glutathione reductase to the samples, fluorescence dropped down only in patient samples and hidden fluorophores appeared. Malignant diseases are associated with the presence of specific metabolites that can be detected fluorescently in biological material such as urine, which can be a suitable alternative for an early detection of cancer or for tracking changes during and after treatment.

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Study of the influence of over-the-counter vitamin supplement intake on urine fluorescence to optimize cancer detection by fluorescence cystoscopy

Cited by 4 publications

References 20 publications

Colorimetric Urinalysis for On-Site Detection of Metabolic Biomarkers

Colorimetric Urinalysis for On-Site Detection of Metabolic Biomarkers

Influence of vitamins and food on the fluorescence spectrum of human urine

Fluorescence biomarkers of malignant melanoma detectable in urine

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