Study of the influence of hyperglycemia on the abundance of amino acids, fatty acids, and selected lipids in extracellular vesicles using TOF-SIMS

Marzec, Magdalena E.; Rząca, Carina; Moskal, P.; Stępień, Ewa

doi:10.1016/j.bbrc.2022.07.020

Cited by 10 publications

(10 citation statements)

References 31 publications

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“…Extracellular vesicles (EVs) are defined as cellular structures surrounded by a protein-lipid membrane secreted by almost all cell types [1][2][3][4][5]. They play an important role in physiological and pathological processes, mediating extracellular transport and transferring various types of intracellular, secretory and membrane proteins, as well as nucleic acids such as small molecule RNA, mRNA, and others [6][7][8][9].…”

Section: Introductionmentioning

confidence: 99%

Comparison of qNANO results from the isolation of extracellular microvesicles with the theoretical model

Durak-Kozica

Wróbel

Platt

et al. 2022

Bio-Algorithms and Med-Systems

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Objectives: Extracellular vesicles (EVs) are heterogeneous membrane vesicles in diameter of 30-5000 nm, that transport proteins, non-coding RNAs (miRNAs), lipids and metabolites. Major populations include exosomes, ectosomes and apoptotic bodies. The purpose of this study was to compare the distribution of EVs obtained under different conditions of differential centrifugation, including ultracentrifugation, with the results developed based on a theoretical model. Methods: Immortalized endothelial cell line that expresses h-TERT (human telomerase) was used to release of EVs: microvascular TIME. EVs were isolated from the culture medium at different centrifugation parameters. The size distribution of the EVs was measured using TRPS technology on a qNano instrument.

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Section: Introductionmentioning

confidence: 99%

Comparison of qNANO results from the isolation of extracellular microvesicles with the theoretical model

Durak-Kozica

Wróbel

Platt

et al. 2022

Bio-Algorithms and Med-Systems

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“…Some of these ionized molecules are characteristic fragments of biomolecules that are components of the tested biological structure, e.g. amino acids, lipids or metabolites, enabling the qualitative characterization of the sample surface [28].…”

Section: Methodsmentioning

confidence: 99%

“…It measures the time of flight of emitted secondary ions through a vacuum tube, including ionized whole biomolecules, ionized fragments of these biomolecules, or newly formed ionized conjugates. Some of these ionized molecules are characteristic fragments of biomolecules that are components of tested biological structure, such as amino acids, lipids or metabolites, which enable the qualitative characterization of the sample surface [27]. It is possible to carry out the described analysis in so-called static mode .…”

Section: Methodsmentioning

confidence: 99%

Revealing sphingolipids composition in extracellular vesicles and paternal β-cells after persistent hyperglycemia

Skalska,

Durak-Kozica,

Stępień

2024

Preprint

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Extended periods of hyperglycemia (HG) can lead to metabolic disorders of sphingolipids (SPs) and their subsequent accumulation in cells. This accumulation can trigger a range of complications, including kidney and neurodegenerative diseases. In our study, we compared the levels of selected ceramides (CER), hexosylceramides (HexCER), and glycosphingolipids (GSLs) in potential biomarkers - extracellular vesicles (EVs). These EVs were derived in vitro from human β-cells cultured under both normoglycemic and high-glucose conditions (HG). We utilized Time of Flight - Secondary Ion Mass Spectrometry (ToF-SIMS) for SP analysis. Our results confirmed that the lipid profiles of these three groups differ between large and small EVs, with some SP lipids being more enriched in EVs compared to cells. Interestingly, our study revealed that HG only regulates the lipid content from the glycosphingolipid group in relation to normoglycemia. Collectively, our findings underscore the potential applications of ToF-SIMS in characterizing the impact of different culture conditions on lipid levels. To the best of our knowledge, our study is the first to employ ToF-SIMS in analyzing the effects of HG on SP levels in EVs and their parental β-cells.

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“…Under normal conditions, PS is usually present in the inner membrane leaflet, but during EV secretion PS is transferred to the outer membrane leaflet. 51 PS is composed of a negatively charged phosphate group attached to the serine at the hydroxyl end. ZP is the measure that indicates the accumulation of negatively charged phospholipids in the inner membrane leaflet.…”

Section: What Do Extracellular Vesicles Contain?mentioning

confidence: 99%

Extracellular vesicles in vascular pathophysiology: beyond their molecular content

Stępień¹,

Durak-Kozica²,

Moskal³

2023

Polish Archives of Internal Medicine

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Extracellular vesicles in vascular pathophysiology 1 Currently, the topic of extracellular vesicles (EVs) and their involvement in disease promotion and progression is gaining important insight in diagnostics and treatment. 5 In this review, we focus on specific EV characteristics, still not intensively investigated, which contribute to the biological activity of EVs, that is, composition of the EV surface called a corona, and its charge.The biological activity of EVs depends on their cargo and their biological availability and biostability. The estimated blood plasma concentration of EVs in healthy individuals is between 10 8 and 10 14 EVs/ml. Such discrepancies, more than 6 orders of magnitude, depend mainly on the isolation protocol and possible contamination by other colloidal particles present in the plasma, such as lipoproteins and large protein aggregates. 6 In total platelet poor plasma, the average platelet microvesicle (PMV) content has been estimated at 10 9 to 10 10 EVs/ml in patients on antiplatelet drugs and healthy individuals. PMV abundance depends mainly on the plasma purity and preanalytical handling. 7-11 Assuming that one -third of the plasma EVs is of platelet origin, the number of the plasma EVs can be approximately 10 10 EVs/ml. 6 The average plasma residence time of intravenously delivered EVs ranges from 30 to 80 minutes and it is mainly regulated by the phagocyting activity of the mononuclear phagocyte system and by Introduction When about 50 years ago Wolf 1 noticed that some tiny and highly abundant objects present in human blood plasma contribute to clotting events, it seemed that there is something beyond clotting factors that may support platelets / thrombocytes in their aggregation and formation of a thrombus. This coagulant particulate material released from platelets (referred to as "platelet dust") was produced in considerably larger amounts than required for thrombin generation. It was detected not only in plasma but also in serum, and its presence seemed to be associated with the platelet -like activity of the serum. 1 The platelet activation leads to secretion of granules containing the procoagulant material and proteins involved in cytoskeletal arrangement, synaptic transportation, and secretion from their internal space. 2 The processes of the formation and secretion of platelet "particles" are analogous to the processes occurring in other cells, including endothelial cells. They result in the formation of 2 types of membrane vesicles: bigger ones shed from the surface and named microvesicles (ectosomes) of 100 nm to 1 µm in diameter, and exosomes, measuring 40 nm to 100 nm in diameter. The latter are similar in size to the internal vesicles in multivesicular bodies (MVBs) and α -granules, 3,4 and can be compared to endothelial vesiculation (FIguRE 1).

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Study of the influence of hyperglycemia on the abundance of amino acids, fatty acids, and selected lipids in extracellular vesicles using TOF-SIMS

Cited by 10 publications

References 31 publications

Comparison of qNANO results from the isolation of extracellular microvesicles with the theoretical model

Comparison of qNANO results from the isolation of extracellular microvesicles with the theoretical model

Revealing sphingolipids composition in extracellular vesicles and paternal β-cells after persistent hyperglycemia

Extracellular vesicles in vascular pathophysiology: beyond their molecular content

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