Study of the isoflavone content of different extracts of Medicago spp. as potential active ingredient

Rodrigues, Francisca; Almeida, Ivone M.C.; Sarmento, Bruno; Amaral, Maria Helena; Oliveira, M. Beatriz P.P.

doi:10.1016/j.indcrop.2014.03.014

Cited by 40 publications

(31 citation statements)

References 41 publications

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“…In addition, all of the most studied isoflavones (genistin, daidzein, glycitein, genistein, formononetin and bioachanin A), thereby with the most well-known activity, were detected in these Medicago species, with special relevance to formononetin. Furthermore, the applied extraction procedure proved to be much more effective than the maceration with polar extracts (Rodrigues et al, 2014). The differences verified for the phenologic indicators may also explain some of the observed variability.…”

Section: Phylogenetic and Phenologic Influence On Isoflavone Profilesmentioning

confidence: 92%

“…Besides the chromatographic data, the proposed identification was also supported by UV spectra as described previously (Rodrigues et al, 2014). Chromatographic parameters, namely limit of detection (LOD), limit of quantification (LOQ), linearity, recovery and repeatability were accepted as previously assessed (Visnevschi-Necrasov et al, 2014a).…”

Section: Phylogenetic and Phenologic Influence On Isoflavone Profilesmentioning

confidence: 99%

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Medicago spp. as potential sources of bioactive isoflavones: Characterization according to phylogenetic and phenologic factors

et al. 2015

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A high variety of plant species are often proposed as potential natural sources of specific bioactive components, with emphasis in phenolic compounds. However, the ability to produce a determined phytochemical might be variable, even among species with close phylogeny. Furthermore, the metabolic dynamics vary greatly according to phenologic factors. Herein, it was verified whether isoflavone production in Medicago spp. is more associated with phylogenetic or phenologic determinants, to define the optimal productive conditions. Isoflavone profiles were characterized in field-grown Medicago species in three phenologic stages. Isoflavones were extracted by matrix solid-phase dispersion method and analyzed using high-performance liquid chromatography coupled with a diode-array detector. The obtained data were evaluated by a generalized linear model (GLM) and linear discriminant analysis (LDA). Formononetin, genistein and irilone were the most abundant isoflavones, reaching values higher than those present in acknowledged plant sources like soy or red clover. Outputs from GLM and LDA indicate that the phylogenetic factors are the most defining criteria. This study promotes Medicago spp. as potential isoflavone sources, particularly because the effects of these compounds are highly dependent on their type and concentration, with potential application as foodstuff, feedstuff, or in the nutraceutical and pharmaceutical industry.

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Section: Phylogenetic and Phenologic Influence On Isoflavone Profilesmentioning

confidence: 92%

Section: Phylogenetic and Phenologic Influence On Isoflavone Profilesmentioning

confidence: 99%

Medicago spp. as potential sources of bioactive isoflavones: Characterization according to phylogenetic and phenologic factors

et al. 2015

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“…Coffea arabica seed oil significantly improves collagen and elastin production, while Coffea robusta has a high concentration of chlorogenic acid (which reduces redness) and caffeine (2). A new tendency in cosmetic formulations is the use of ingredients obtained from food by-products discarded as waste, answering to sustainability concerns (3)(4)(5)(6)(7). Coffee silverskin (CS) is a coffee roasting by-product with no commercial value.…”

Section: Introductionmentioning

confidence: 99%

In vitro and in vivo comparative study of cosmetic ingredients Coffee silverskin and hyaluronic acid

Rodrigues

Matias²,

Ferreira³

et al. 2016

Experimental Dermatology

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Additional Supporting Information may be found online in the supporting information tab for this article:Data S1 Supplementary Material, Methods and References.Figure S1 Erdr1 is significantly decreased in psoriatic lesional skin. Figure S2 Expression of Erdr1 increases after treatment using cyclosporin A (CsA) in psoriasis-like skin inflammation mouse model. Figure S3 Recombinant Erdr1 exerts anti-inflammatory properties in a psoriasis-like skin inflammation mouse model. BackgroundFacial wrinkles are one of the major features of ageing. Traditionally, hyaluronic acid (HA) is used as a potent antiwrinkle compound. HyaCare â Filler CL (Evonik Industries AG, Essen, Germany) is a new anti-ageing ingredient with a cross-linked polysaccharide made from fermentation-derived HA, contributing to the reduction in facial fine lines and wrinkles as well as increasing skin elasticity and hydration (1). Recently, the same purposes were made for caffeine. Extracts from two species of coffee directly combat UV damage improving strength, resilience and elasticity of facial skin (2). Coffea arabica seed oil significantly improves collagen and elastin production, while Coffea robusta has a high concentration of chlorogenic acid (which reduces redness) and caffeine (2). A new tendency in cosmetic formulations is the use of ingredients obtained from food by-products discarded as waste, answering to sustainability concerns (3-7). Coffee silverskin (CS) is a coffee roasting by-product with no commercial value. According to the International Coffee Organization (ICO), in 2014, the world coffee production has been around 141 620 million bags of 60 kg, which means that about 3 823 740 kg of these wastes were produced. Recent studies reveal its antioxidant potential, caffeine content and low cytotoxicity (6). Rodrigues et al. evaluated the CS skin and ocular irritation potential, using reconstructed human epidermis, EpiSkin TM (SkinEthics Laboratories, Lyon, France), and human corneal epithelial model, SkinEthics TM HCE (SkinEthics Laboratories, Lyon, France), respectively (7). Results demonstrated that CS is not irritant and the in vivo assay revealed that extract can be regarded as safe for topical application (7). Questions addressed and experimental design ResultsFranz diffusion cells were used and pig ear skin selected as model barrier, to evaluate the caffeine permeation. Results are presented in Fig. 1. After 8 h, a very small amount of caffeine crossed the skin, respectively, about 20% of the extract content. The extract improves skin hydration after 48 h of a patch test when compared to the negative control used (P < 0.05) (Table S1). Probably, this effect is due to the well-detailed extract composition (6,7). For keratinocyte (HaCaT) and fibroblast (HFF-1), no cytotoxicity was observed for the different concentrations tested (1, 5 and 10 mg/ml), which means that these formulations are potentially safe (Table S2). In vivo evaluationStandardized clinical photographs were taken at baseline and at 28 days using a Visioface â (Cou...

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“…According to the composition previously reported for all olive by-products, in an environmental and economic point of view, they could be considered a promising cosmetic ingredient source. Nevertheless, to guarantee the safety of these new ingredients stability and toxicity assays should be performed in order to avoid the presence of irritant constituents (Rodrigues et al, 2014). The citotoxicity of these compounds can be evaluated using MTS and LDH assays in different monolayer's skin cell lines.…”

Section: Antioxidant Activitymentioning

confidence: 99%

“…The similarity of virgin olive oil composition to sebum, given by its high content of squalene and ␤-sitosterol, optimum fatty acids profile (rich in oleic acid, a skin softener), makes it particularly able to directly protect the skin (Viola and Viola, 2009). However, very few studies reported the use of by-products as antioxidant sources for cosmetic field (Rodrigues et al, 2014(Rodrigues et al, , 2015(Rodrigues et al, , 2013. According to these researches, the majority of these antioxidants are polyphenols and isoflavones.…”

Section: Antioxidant Activitymentioning

confidence: 99%

Olive by-products: Challenge application in cosmetic industry

Rodrigues

Pimentel

Oliveira

2015

Industrial Crops and Products

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183

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Study of the isoflavone content of different extracts of Medicago spp. as potential active ingredient

Cited by 40 publications

References 41 publications

Medicago spp. as potential sources of bioactive isoflavones: Characterization according to phylogenetic and phenologic factors

Medicago spp. as potential sources of bioactive isoflavones: Characterization according to phylogenetic and phenologic factors

In vitro and in vivo comparative study of cosmetic ingredients Coffee silverskin and hyaluronic acid

Olive by-products: Challenge application in cosmetic industry

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