Study of the Mechanical Behavior of Subcellular Organelles Using a 3D Finite Element Model of the Tensegrity Structure

Khunsaraki, Gholamreza Mohammadi; Oscuii, Hanieh Niroomand; Voloshin, Arkady

doi:10.3390/app11010249

Cited by 6 publications

(9 citation statements)

References 29 publications

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“…FEA also allows for investigation of intra-cellular and extra-cellular forces at the pN (10 −12 N) level. 38 Atomic force microscopy results have validated such computations.…”

Section: Finite Element Analysis and Other Computational Biomechanical Toolsmentioning

confidence: 75%

Basic Biomechanics for Craniofacial Surgeons: The Responses of Alloplastic Materials and Living Tissues to Mechanical Forces

Buchman

Gosain

et al. 2021

FACE

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Many terms such as twist, compress, bend, and stretch, describe how materials behave when subjected to mechanical stresses. Subjective adjectives to describe the property of materials such as hard or brittle are imprecise and impedes proper understanding of important principles needed in planning and performing surgical treatments. The viability of tissue and time dependent variables effect healing and compound the issue. Some parameters are time dependent (strain rate), while others are nearly independent of time (Young’s modulus). The craniofacial skeleton and enveloping soft tissues are viscoelastic composite materials which undergo time-dependent changes upon loading. The ability to remodel and respond to environmental changes makes them “smart,” reenforcing where needed and removing where not required based on a set of predetermined upper and lower thresholds. This mini review has 7 sections on engineering principles that underpin craniofacial surgery: (1) The general concept of mechanics: load, force, stress, strain, compression, tension, shear, stress-strain curves and values derived from them such as Young’s modulus, fatigue damage, and load- shearing. (2) Material properties of bone and suture and structural engineering of the craniofacial skeleton in normal and pathological conditions. (3) Fixation using wires, screws, and plates: anatomy and function of screws and plates, locking plates, lag screws, internal and external fixators. (4) Biomechanics of distraction osteogenesis and the effects of radiation. (5) Finite element analysis and other computational biomechanical tools. (6) Virtual surgical planning, cutting guides, and intra-operative navigation. (7) Tissue engineering: design goals, criteria, and constraints. An appreciation and understanding of these biomechanical principles will help craniofacial surgeons to facilitate intrinsic optimization and better treat complex morphological problems, helping one achieve the most favorable and durable results. The biological responses to mechanical stress are extremely important as well, but due to space constraints, they will be the subject of a separate dedicated review.

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“…FEA also allows for investigation of intra-cellular and extra-cellular forces at the pN (10 −12 N) level. 38 Atomic force microscopy results have validated such computations.…”

Section: Finite Element Analysis and Other Computational Biomechanical Toolsmentioning

confidence: 75%

Basic Biomechanics for Craniofacial Surgeons: The Responses of Alloplastic Materials and Living Tissues to Mechanical Forces

Buchman

Gosain

et al. 2021

FACE

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“…The curves from the work of Baaijens et al . 25 are compared to the Half-Space analytical model (dashed line) and to our simulation (red line with stars). The cell diameter is fixed.…”

Section: Resultsmentioning

confidence: 99%

“…Data from the work of Baaijens et al . 25 are reported, compared with our simulation (red line with stars), and the Half-Space model with a dashed blue line. (c) Comparison of simulations of the loading phase of the micropipette aspiration employing different D c /D p ratios.…”

Section: Resultsmentioning

confidence: 99%

“…In Barreto et al 3 the importance of actin filaments and microtubules during cell compression was highlighted, and in Katti et al 24 they observed that a decrease in the mechanical properties of the cytoskeleton dramatically influences the global cell behaviour. In addition, from Khunsaraki et al 25 , they observed that the cytoskeleton is the most involved part to carry the reaction force during the AFM tip indentation. From our insights, we can also state that cytoplasm is able to influence the global mechanical response of a cell undergoing AFM indentation, since its variation led to significant different behaviours of the cell (Fig.…”

Section: Discussionmentioning

confidence: 99%

“…For this reason, in-silico computational models have been developed through the years, to overcome such limitations and uncertainties, while proposing a valuable tool to understand cell mechanical behaviour. 1 – 3 , 5 , 24 , 28 , 32 , 41 Some of these models describe the cell as an homogeneous viscoelastic material, 1 – 3 , 41 others specify the main subcomponents adopting a tensegrity structure to model the cytoskeleton, 5 , 24 , 25 , 28 as stated and assessed in past works. 22 , 23 , 40…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

A Continuum-Tensegrity Computational Model for Chondrocyte Biomechanics in AFM Indentation and Micropipette Aspiration

et al. 2022

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Mechanical stimuli are fundamental in the development of organs and tissues, their growth, regeneration or disease. They influence the biochemical signals produced by the cells, and, consequently, the development and spreading of a disease. Moreover, tumour cells are usually characterized by a decrease in the cell mechanical properties that may be directly linked to their metastatic potential. Thus, recently, the experimental and computational study of cell biomechanics is facing a growing interest. Various experimental approaches have been implemented to describe the passive response of cells; however, cell variability and complex experimental procedures may affect the obtained mechanical properties. For this reason, in-silico computational models have been developed through the years, to overcome such limitations, while proposing valuable tools to understand cell mechanical behaviour. This being the case, we propose a combined continuous-tensegrity finite element (FE) model to analyse the mechanical response of a cell and its subcomponents, observing how every part contributes to the overall mechanical behaviour. We modelled both Atomic Force Microscopy (AFM) indentation and micropipette aspiration techniques, as common mechanical tests for cells and elucidated also the role of cell cytoplasm and cytoskeleton in the global cell mechanical response.

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Discrete network models of endothelial cells and their interactions with the substrate

Jakob,

Britt,

Giampietro

et al. 2024

Biomech Model Mechanobiol

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Endothelial cell monolayers line the inner surfaces of blood and lymphatic vessels. They are continuously exposed to different mechanical loads, which may trigger mechanobiological signals and hence play a role in both physiological and pathological processes. Computer-based mechanical models of cells contribute to a better understanding of the relation between cell-scale loads and cues and the mechanical state of the hosting tissue. However, the confluency of the endothelial monolayer complicates these approaches since the intercellular cross-talk needs to be accounted for in addition to the cytoskeletal mechanics of the individual cells themselves. As a consequence, the computational approach must be able to efficiently model a large number of cells and their interaction. Here, we simulate cytoskeletal mechanics by means of molecular dynamics software, generally suitable to deal with large, locally interacting systems. Methods were developed to generate models of single cells and large monolayers with hundreds of cells. The single-cell model was considered for a comparison with experimental data. To this end, we simulated cell interactions with a continuous, deformable substrate, and computationally replicated multistep traction force microscopy experiments on endothelial cells. The results indicate that cell discrete network models are able to capture relevant features of the mechanical behaviour and are thus well-suited to investigate the mechanics of the large cytoskeletal network of individual cells and cell monolayers.

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Study of the Mechanical Behavior of Subcellular Organelles Using a 3D Finite Element Model of the Tensegrity Structure

Cited by 6 publications

References 29 publications

Basic Biomechanics for Craniofacial Surgeons: The Responses of Alloplastic Materials and Living Tissues to Mechanical Forces

Basic Biomechanics for Craniofacial Surgeons: The Responses of Alloplastic Materials and Living Tissues to Mechanical Forces

A Continuum-Tensegrity Computational Model for Chondrocyte Biomechanics in AFM Indentation and Micropipette Aspiration

Discrete network models of endothelial cells and their interactions with the substrate

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