Study of the metabolism of myricetin in rat urine, plasma and feces by ultra‐high‐performance liquid chromatography

Li, Huajian; Li, Haoran; Jiang, Shan; Xu, Jun; Cui, Yifang; Wang, Hong; Dai, Long; Lin, Yanping; Zhang, Jiayu

doi:10.1002/bmc.5281

Cited by 3 publications

(1 citation statement)

References 30 publications

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“…The clinical symptoms included manifested as scratching the injection site, decreased diet and mental depression. The mice were orally treated with 100 mg/kg myricetin twice a day because the oral elimination half-life in rats was only 3.53 h ( Li et al, 2021 ). After myricetin treatment, the survival time of the mice was prolonged; the pathological damages caused by infection were also relieved; the proliferation of PRV was also inhibited in each organ ( Figures 7A – D ).…”

Section: Discussionmentioning

confidence: 99%

Myricetin inhibits pseudorabies virus infection through direct inactivation and activating host antiviral defense

Zhang

et al. 2022

Front. Microbiol.

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Myricetin, a polyhydroxyflavone compound, is one of the main ingredients of various human foods and therefore also known as dietary flavonoids. Due to the continuous emergence of resistant strains of herpesviruses, novel control measures are required. In the present study, myricetin exhibited potent antiviral activity against pseudorabies virus (PRV), a model organism of herpesvirus. The suppression rate could reach up to 96.4% at a concentration of 500 μM in cells, and the 50% inhibitory concentration (IC 50 ) was 42.69 μM. Moreover, the inhibitory activity was not attenuated by the increased amount of infective dose, and a significant reduction of intracellular PRV virions was observed by indirect immunofluorescence. A mode of action study indicated that myricetin could directly inactivate the virus in vitro, leading to inhibition of viral adsorption, penetration and replication in cells. In addition to direct killing effect, myricetin could also activate host antiviral defense through regulation of apoptosis-related gene expressions (Bcl-2, Bcl-xl, Bax), NF-κB and MAPK signaling pathways and cytokine gene expressions (IL-1α, IL-1β, IL-6, c-Jun, STAT1, c-Fos, and c-Myc). In PRV-infected mouse model, myricetin could enhance the survival rate by 40% at 5 days post infection, and viral loads in kidney, liver, lung, spleen, and brain were significantly decreased. The pathological changes caused by PRV infection were improved by myricetin treatment. The gene expressions of inflammatory factors (MCP-1, G-CSF, IL-1α, IL-1β, and IL-6) and apoptotic factors (Bcl-xl, Bcl-2, and Bax) were regulated by myricetin in PRV-infected mice. The present findings suggest that myricetin can effectively inhibit PRV infection and become a candidate for development of new anti-herpesvirus drugs.

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Section: Discussionmentioning

confidence: 99%

Myricetin inhibits pseudorabies virus infection through direct inactivation and activating host antiviral defense

Zhang

et al. 2022

Front. Microbiol.

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Research Progress on Flavonoids and Alkaloids in <i>Portulaca oleracea</i> L.

李

2023

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Comprehensive Study of In vivo and In vitro Metabolites of Cycloastragenol Based on UHPLC-Q-Exactive Orbitrap Mass Spectrometer

Wang

et al. 2022

CDM

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Background: Cycloastragenol (CAG) is a sapogenin derived from the main bioactive constituents of Astragali Radix (AR). However, the current research on CAG metabolism in vivo and in vitro is still inadequate, and the metabolite cluster is incomplete due to incomplete analysis strategy. background: Cycloastragenol (CAG) is a sapogenin derived from the main bioactive constituents in Astragali Radix (AR). However, the current research on CAG metabolism in vivo and in vitro is still inadequate, or the metabolite cluster was incomplete due to the incomplete analysis strategy. Objective: The objective of this study was to screen and identify the metabolism behavior of CAG in vivo and in vitro. Objective: The objective of this study was to screen and identify the metabolic behavior of CAG in vivo and in vitro. objective: The objective of this study was to screen and identify the metabolism behavior of CAG in vivo and in vitro. Methods: A simple and rapid analysis strategy based on UHPLC-Q-Exactive Orbitrap mass spectrometry combined with data-mining processing technology was developed and used to screen and identify CAG metabolites in rat body fluids and tissues after oral administration. method: A simple and rapid system strategy based on UHPLC-Q-Exactive Orbitrap mass spectrometry combined with data-mining processing technology was developed and used to screen and identify CAG metabolites in rat body fluids and tissues after oral administration. Results: As a result, a total of 82 metabolites were fully or partially characterized based on their accurate mass, characteristic fragment ions, retention times, corresponding Clog P values, and so on. Among the metabolites, 61 were not reported in previous reports. These metabolites (6 metabolites in vitro and 91 in vivo) were generated through reactions of hydroxylation, glucuronidation, sulfation, hydrogenation, hydroxylation, demethylation, disopropylation, dehydroxylation, ring cleavage, and carboxyl substitution and their composite reactions, and the hydroxylation might be the main metabolic reaction of CAG. In addition, the characteristic fragmentation pathways of CAG were summarized for the subsequent metabolite identification. result: A total of 82 metabolites were fully or partially characterized based on their accurate mass, characteristic fragment ions, retention times, corresponding Clog P values, and so on. As a result, these metabolites (6 metabolites in vitro and 91 in vivo) were presumed to generate through mono- to tri-hydroxylation, glucuronidation, sulfation, hydrogenation, hydroxylation, demethylation, deisopropylation, dehydroxylation, ring cleavage, and carboxyl substitution and their composite reactions. In addition, the characteristic fragmentation pathways of CAG were summarized for the subsequent metabolite identification. Conclusion: The current study not only clarifies the metabolite cluster-based and metabolic regularity of CAG in vivo and in vitro, but also provides ideas for metabolism of other saponin compounds. other: no

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Study of the metabolism of myricetin in rat urine, plasma and feces by ultra‐high‐performance liquid chromatography

Cited by 3 publications

References 30 publications

Myricetin inhibits pseudorabies virus infection through direct inactivation and activating host antiviral defense

Myricetin inhibits pseudorabies virus infection through direct inactivation and activating host antiviral defense

Research Progress on Flavonoids and Alkaloids in <i>Portulaca oleracea</i> L.

Comprehensive Study of In vivo and In vitro Metabolites of Cycloastragenol Based on UHPLC-Q-Exactive Orbitrap Mass Spectrometer

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