In conscious hens, the effect of intra-aortic infusion of prostaglandin E2 (PGE2) on myoelectrical activity of the uterus, utero-vaginal junction (UVJ), vagina, duodenum, and on heart rate was studied. Infusion of .3 micrograms PGE2/animal/min resulted in no significant changes in oviduct activity, whereas 3 micrograms/animal/min induced a significant decrease in vaginal activity but had no significant effect on activity of the uterus and UVJ. Infusion of both doses provoked a decrease in duodenal activity (P less than .05) and an increase in heart rate (P less than .05).